Expression of the human germinal center-associated lymphoma (HGAL) protein, a new marker of germinal center B-cell derivation

Yasodha Natkunam, Izidore Lossos, Behnaz Taidi, Shuchun Zhao, XiaoQing Lu, Feying Ding, Anne Sofie Hammer, Teresa Marafioti, Gerald E. Byrne, Shoshana Levy, Roger A. Warnke, Ronald Levy

Research output: Contribution to journalArticle

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Abstract

We identified the human germinal center-associated lymphoma (HGAL) in gene-expression profiling studies of diffuse large B-cell lymphoma (DLBCL). The expression of HCAL correlated with survival in patients with DLBCL. The HGAL gene is the human homolog of M17, a mouse gene expressed specifically in normal germinal center (GC) B cells. We generated a monoclonal antibody against the HGAL protein and show that HGAL is expressed in the cytoplasm of GC lymphocytes and in lymphomas of GC derivation. Among 727 lymphomas tested by immunohistochemistry on tissue microarrays, HGAL staining was found in follicular lymphomas (103 of 107), Burkitt lymphomas (40 of 40), mediastinal large B lymphomas (7 of 8), and in DLBCLs (103 of 151). Most marginal zone lymphomas lacked HGAL staining. Lymphocyte-predominant Hodgkin lymphomas (12 of 17) and, surprisingly, classical Hodgkin lymphomas (78 of 107) were found to be positive. Hierarchical clustering of comparative immunohistologic results in DLBCLs demonstrates that the expression of HGAL is similar to 2 other GC-associated proteins, BCL6 and CD10, but different from 2 markers associated with a non-GC phenotype, MUM1/IRF4 and BCL2. The restricted expression and GC specificity of HGAL protein suggest that it may have an important role in the diagnosis of specific lymphomas, and, potentially in the identification of sub-types associated with different prognoses.

Original languageEnglish
Pages (from-to)3979-3986
Number of pages8
JournalBlood
Volume105
Issue number10
DOIs
StatePublished - May 15 2005
Externally publishedYes

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Germinal Center
Lymphocytes
Lymphoma
B-Lymphocytes
Genes
Cells
Microarrays
Gene expression
Monoclonal Antibodies
Tissue
Lymphoma, Large B-Cell, Diffuse
Proteins
Hodgkin Disease
human GCET2 protein
Staining and Labeling
Follicular Lymphoma
Burkitt Lymphoma
Gene Expression Profiling
Cluster Analysis
Cytoplasm

ASJC Scopus subject areas

  • Hematology

Cite this

Expression of the human germinal center-associated lymphoma (HGAL) protein, a new marker of germinal center B-cell derivation. / Natkunam, Yasodha; Lossos, Izidore; Taidi, Behnaz; Zhao, Shuchun; Lu, XiaoQing; Ding, Feying; Hammer, Anne Sofie; Marafioti, Teresa; Byrne, Gerald E.; Levy, Shoshana; Warnke, Roger A.; Levy, Ronald.

In: Blood, Vol. 105, No. 10, 15.05.2005, p. 3979-3986.

Research output: Contribution to journalArticle

Natkunam, Y, Lossos, I, Taidi, B, Zhao, S, Lu, X, Ding, F, Hammer, AS, Marafioti, T, Byrne, GE, Levy, S, Warnke, RA & Levy, R 2005, 'Expression of the human germinal center-associated lymphoma (HGAL) protein, a new marker of germinal center B-cell derivation', Blood, vol. 105, no. 10, pp. 3979-3986. https://doi.org/10.1182/blood-2004-08-3112
Natkunam, Yasodha ; Lossos, Izidore ; Taidi, Behnaz ; Zhao, Shuchun ; Lu, XiaoQing ; Ding, Feying ; Hammer, Anne Sofie ; Marafioti, Teresa ; Byrne, Gerald E. ; Levy, Shoshana ; Warnke, Roger A. ; Levy, Ronald. / Expression of the human germinal center-associated lymphoma (HGAL) protein, a new marker of germinal center B-cell derivation. In: Blood. 2005 ; Vol. 105, No. 10. pp. 3979-3986.
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AU - Lu, XiaoQing

AU - Ding, Feying

AU - Hammer, Anne Sofie

AU - Marafioti, Teresa

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