Expression of the DeaD RNA Helicase Is Regulated at Multiple Levels through Its Long mRNA 59 Untranslated Region

Sandeep Ojha, Chaitanya Jain

Research output: Contribution to journalArticlepeer-review

Abstract

DEAD-box proteins (DBPs) are a prominent class of RNA remodeling proteins that alter RNA structure, a process they typically perform through an ATP-dependent RNA helicase activity. Although many DBPs have been characterized at the structural and functional level in detail, much less is known about how they are regulated. We previously showed that the mRNA for the Escherichia coli DeaD DBP contains an unusually long 59 untranslated region (59 UTR) of 838 nucleotides (nt) and that it is the primary RNA determinant of DeaD autoregulation. We speculated that such a long and complex 59 UTR might regulate deaD expression in additional ways. Here, we show that the deaD mRNA 59 UTR regulates deaD expression at two additional levels, temperature-dependent expression and through a stem-loop structure overlapping the start codon. These results support the hypothesis that a long 59 UTR can regulate gene expression through multiple mechanisms.

Original languageEnglish (US)
Article numbere00613-21
JournalJournal of bacteriology
Volume204
Issue number3
DOIs
StatePublished - Mar 2022
Externally publishedYes

Keywords

  • 59 untranslated region
  • DeaD-box proteins
  • RNA helicase
  • RNA structure
  • Regulation of gene expression

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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