Expression of the costimulatory B7-1 molecule prevents immune privilege in the anterior chamber of the eye

B. R. Ksander, S. Miki, C. Geer, J. W. Streilein, E. Podack

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Abstract

Tumor cells injected into the immunologically privileged anterior chamber (AC) of the eye grow progressively and induce an abnormal immune response (ACAID) that allows immunogenic tumor cells to escape immune-mediated elimination. The following experiments were performed to determine if the expression of the costimulatory B7-1 molecule on P815 tumor cells prevents immune privilege and progressive tumor growth within the AC of BALB/c mice. To this end, P815 cells were transfected with the episomal vector pBMGNeo that contained murine B7-1 cDNA. Transfected P815 cells (P815-B7-1+) constitutively expressed B7-1 on the cell surface. Groups of BALB/c mice received AC inoculations of P815-B7-1+ cells, and tumor growth was determined quantitatively by slit-lamp examination. Two types of negative controls were used in these experiments: 1) untransfected P815 cells, and 2) P815 cells transfected with empty pBMGNeo vectors that did not contain B7-1 cDNA. The P815-B7-1+ tumor cells grew progressively, and filled 100% of the AC by day 10 post-inoculation. During this time, the growth of B7-1+ tumor cells was not significantly different from the growth of either untransfected P815 cells or P815 cells transfected with empty pBMGNeo vectors. By contrast, between days 10 and 21 post-inoculation, P815-B7-1+ cells were eliminated completely, while tumors in control mice continued to grow progressively. Elimination of B7-1+ tumor cells was dependent upon T and/or B cells, as demonstrated by the growth of B7-1+ tumors in the AC of SCID mice. We conclude from these experiments that the expression of B7-1+ on P815 tumor cells prevents tumor cells from enjoying immune privilege within the anterior chamber of the eye.

Original languageEnglish (US)
Pages (from-to)128-133
Number of pages6
JournalRegional Immunology
Volume6
Issue number1-2
StatePublished - Dec 1 1994

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ASJC Scopus subject areas

  • Immunology

Cite this

Ksander, B. R., Miki, S., Geer, C., Streilein, J. W., & Podack, E. (1994). Expression of the costimulatory B7-1 molecule prevents immune privilege in the anterior chamber of the eye. Regional Immunology, 6(1-2), 128-133.