Expression of the Argonaute protein PiwiL2 and piRNAs in adult mouse mesenchymal stem cells

Qiuling Wu, Qi Ma, Lina Shehadeh, Amber Wilson, Linghui Xia, Hong Yu, Keith A Webster

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Piwi (P-element-induced wimpy testis) first discovered in Drosophila is a member of the Argonaute family of micro-RNA binding proteins with essential roles in germ-cell development. The murine homologue of PiwiL2, also known as Mili is selectively expressed in the testes, and mice bearing targeted mutations of the PiwiL2 gene are male-sterile. PiwiL2 proteins are thought to protect the germ line genome by suppressing retrotransposons, stabilizing heterochromatin structure, and regulating target genes during meiosis and mitosis. Here, we report that PiwiL2 and associated piRNAs (piRs) may play similar roles in adult mouse mesenchymal stem cells. We found that PiwiL2 is expressed in the cytoplasm of metaphase mesenchymal stem cells from the bone marrow of adult and aged mice. Knockdown of PiwiL2 with a specific siRNA enhanced cell proliferation, significantly increased the number of cells in G1/S and G2/M cell cycle phases and was associated with increased expression of cell cycle genes CCND1, CDK8, microtubule regulation genes, and decreased expression of tumor suppressors Cables 1, LATS, and Cxxc4. The results suggest broader roles for Piwi in genome surveillance beyond the germ line and a possible role in regulating the cell cycle of mesenchymal stem cells.

Original languageEnglish
Pages (from-to)915-920
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume396
Issue number4
DOIs
StatePublished - Jun 11 2010

Fingerprint

Argonaute Proteins
Stem cells
Mesenchymal Stromal Cells
Germ Cells
Testis
Genes
Cell Cycle
Long-Acting Thyroid Stimulator
Genome
Cells
cdc Genes
Retroelements
RNA-Binding Proteins
Heterochromatin
Meiosis
Gene Expression Regulation
Metaphase
MicroRNAs
Mitosis
Microtubules

Keywords

  • Cell cycle
  • Cell division
  • miRNA
  • piRNA
  • Stem cell
  • Stromal cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Expression of the Argonaute protein PiwiL2 and piRNAs in adult mouse mesenchymal stem cells. / Wu, Qiuling; Ma, Qi; Shehadeh, Lina; Wilson, Amber; Xia, Linghui; Yu, Hong; Webster, Keith A.

In: Biochemical and Biophysical Research Communications, Vol. 396, No. 4, 11.06.2010, p. 915-920.

Research output: Contribution to journalArticle

Wu, Qiuling ; Ma, Qi ; Shehadeh, Lina ; Wilson, Amber ; Xia, Linghui ; Yu, Hong ; Webster, Keith A. / Expression of the Argonaute protein PiwiL2 and piRNAs in adult mouse mesenchymal stem cells. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 396, No. 4. pp. 915-920.
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