Expression of the β1 chain (CD29) of integrins and CD45 in alcoholic liver disease

A. Chedid, C. L. Mendenhall, T. E. Moritz, S. W. French, T. S. Chen, T. R. Morgan, D. Day, N. E. Fricke, L. Rittmeier, M. Lucente, C. J. McClain, G. A. Roselle, Eugene R Schiff, P. L. Day, W. G. Henderson, R. E. Weesner, D. V. Trulzsch, E. Pinnow, J. Nelson

Research output: Contribution to journalArticle

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Abstract

Objectives: In the active stages of alcoholic liver disease (ALD), we have shown previously an enhanced expression of either CD4 or CD8 molecules on CD3+T cells. Class I and II MHC molecules expression were enhanced on lymphocytes, and on the cell membrane of hepatocytes, thus suggesting that an intrahepatic antigen-dependent lymphocyte-hepatocyte interaction occurs. Here we report the pattern of expression of several additional molecules known to participate in nonspecific cell-cell adhesion preceding alloantigen recognition i.e., the β1 chain of integrins (CD29) and the isoforms of the leukocyte common antigen CD45RA and CD45RO. Methods: Frozen liver samples from 38 patients with advanced ALD were examined immunohistochemically by the avidin-biotin-peroxidase complex method using monoclonal antibodies. Nine patients with alcoholic fatty liver and six patients with only hepatitis C infection were included as controls and studied in a similar manner. Results: CD29 was strongly expressed on the cell membrane of hepatocytes in 18/20 patients with cirrhosis, in 17/18 with alcoholic hepatitis without cirrhosis, in 5/9 of fatty liver and in all six with hepatitis C. CD45RA was present in 5/18 cases of alcoholic hepatitis and in 3/20 cases of cirrhosis on the hepatocytes plasma membrane, in none of the controls, and rarely on lymphocytes. CD45RO was expressed on the surface of lymphocytes in 14/18 cases of alcoholic hepatitis, 13/20 patients with cirrhosis, in 5/9 of fatty liver and in one with hepatitis C. The CD45RO lymphocytes were predominantly CD8 positive. Conclusions: Our results indicate that in ALD the hepatocytes exhibit on their plasma membrane an enhanced expression of the β1 chain of the integrins and less commonly CD45RA. Furthermore, the expression of only CD45RO on the surface of lymphocytes favors the postulate that the intrahepatic lymphocytes in ALD are most likely of the 'memory' type. The results of this study lend further support to the contention that a cell- cell contact precedes a cell-mediated mechanism in the pathogenesis of ALD.

Original languageEnglish
Pages (from-to)1920-1927
Number of pages8
JournalAmerican Journal of Gastroenterology
Volume88
Issue number11
StatePublished - Jan 1 1993
Externally publishedYes

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Alcoholic Liver Diseases
Integrins
Lymphocytes
Hepatocytes
Alcoholic Hepatitis
Fibrosis
Hepatitis C
Cell Membrane
Fatty Liver
Alcoholic Fatty Liver
CD45 Antigens
Isoantigens
Avidin
Biotin
Cell Adhesion
Peroxidase
Protein Isoforms
Monoclonal Antibodies
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Chedid, A., Mendenhall, C. L., Moritz, T. E., French, S. W., Chen, T. S., Morgan, T. R., ... Nelson, J. (1993). Expression of the β1 chain (CD29) of integrins and CD45 in alcoholic liver disease. American Journal of Gastroenterology, 88(11), 1920-1927.

Expression of the β1 chain (CD29) of integrins and CD45 in alcoholic liver disease. / Chedid, A.; Mendenhall, C. L.; Moritz, T. E.; French, S. W.; Chen, T. S.; Morgan, T. R.; Day, D.; Fricke, N. E.; Rittmeier, L.; Lucente, M.; McClain, C. J.; Roselle, G. A.; Schiff, Eugene R; Day, P. L.; Henderson, W. G.; Weesner, R. E.; Trulzsch, D. V.; Pinnow, E.; Nelson, J.

In: American Journal of Gastroenterology, Vol. 88, No. 11, 01.01.1993, p. 1920-1927.

Research output: Contribution to journalArticle

Chedid, A, Mendenhall, CL, Moritz, TE, French, SW, Chen, TS, Morgan, TR, Day, D, Fricke, NE, Rittmeier, L, Lucente, M, McClain, CJ, Roselle, GA, Schiff, ER, Day, PL, Henderson, WG, Weesner, RE, Trulzsch, DV, Pinnow, E & Nelson, J 1993, 'Expression of the β1 chain (CD29) of integrins and CD45 in alcoholic liver disease', American Journal of Gastroenterology, vol. 88, no. 11, pp. 1920-1927.
Chedid A, Mendenhall CL, Moritz TE, French SW, Chen TS, Morgan TR et al. Expression of the β1 chain (CD29) of integrins and CD45 in alcoholic liver disease. American Journal of Gastroenterology. 1993 Jan 1;88(11):1920-1927.
Chedid, A. ; Mendenhall, C. L. ; Moritz, T. E. ; French, S. W. ; Chen, T. S. ; Morgan, T. R. ; Day, D. ; Fricke, N. E. ; Rittmeier, L. ; Lucente, M. ; McClain, C. J. ; Roselle, G. A. ; Schiff, Eugene R ; Day, P. L. ; Henderson, W. G. ; Weesner, R. E. ; Trulzsch, D. V. ; Pinnow, E. ; Nelson, J. / Expression of the β1 chain (CD29) of integrins and CD45 in alcoholic liver disease. In: American Journal of Gastroenterology. 1993 ; Vol. 88, No. 11. pp. 1920-1927.
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abstract = "Objectives: In the active stages of alcoholic liver disease (ALD), we have shown previously an enhanced expression of either CD4 or CD8 molecules on CD3+T cells. Class I and II MHC molecules expression were enhanced on lymphocytes, and on the cell membrane of hepatocytes, thus suggesting that an intrahepatic antigen-dependent lymphocyte-hepatocyte interaction occurs. Here we report the pattern of expression of several additional molecules known to participate in nonspecific cell-cell adhesion preceding alloantigen recognition i.e., the β1 chain of integrins (CD29) and the isoforms of the leukocyte common antigen CD45RA and CD45RO. Methods: Frozen liver samples from 38 patients with advanced ALD were examined immunohistochemically by the avidin-biotin-peroxidase complex method using monoclonal antibodies. Nine patients with alcoholic fatty liver and six patients with only hepatitis C infection were included as controls and studied in a similar manner. Results: CD29 was strongly expressed on the cell membrane of hepatocytes in 18/20 patients with cirrhosis, in 17/18 with alcoholic hepatitis without cirrhosis, in 5/9 of fatty liver and in all six with hepatitis C. CD45RA was present in 5/18 cases of alcoholic hepatitis and in 3/20 cases of cirrhosis on the hepatocytes plasma membrane, in none of the controls, and rarely on lymphocytes. CD45RO was expressed on the surface of lymphocytes in 14/18 cases of alcoholic hepatitis, 13/20 patients with cirrhosis, in 5/9 of fatty liver and in one with hepatitis C. The CD45RO lymphocytes were predominantly CD8 positive. Conclusions: Our results indicate that in ALD the hepatocytes exhibit on their plasma membrane an enhanced expression of the β1 chain of the integrins and less commonly CD45RA. Furthermore, the expression of only CD45RO on the surface of lymphocytes favors the postulate that the intrahepatic lymphocytes in ALD are most likely of the 'memory' type. The results of this study lend further support to the contention that a cell- cell contact precedes a cell-mediated mechanism in the pathogenesis of ALD.",
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T1 - Expression of the β1 chain (CD29) of integrins and CD45 in alcoholic liver disease

AU - Chedid, A.

AU - Mendenhall, C. L.

AU - Moritz, T. E.

AU - French, S. W.

AU - Chen, T. S.

AU - Morgan, T. R.

AU - Day, D.

AU - Fricke, N. E.

AU - Rittmeier, L.

AU - Lucente, M.

AU - McClain, C. J.

AU - Roselle, G. A.

AU - Schiff, Eugene R

AU - Day, P. L.

AU - Henderson, W. G.

AU - Weesner, R. E.

AU - Trulzsch, D. V.

AU - Pinnow, E.

AU - Nelson, J.

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Y1 - 1993/1/1

N2 - Objectives: In the active stages of alcoholic liver disease (ALD), we have shown previously an enhanced expression of either CD4 or CD8 molecules on CD3+T cells. Class I and II MHC molecules expression were enhanced on lymphocytes, and on the cell membrane of hepatocytes, thus suggesting that an intrahepatic antigen-dependent lymphocyte-hepatocyte interaction occurs. Here we report the pattern of expression of several additional molecules known to participate in nonspecific cell-cell adhesion preceding alloantigen recognition i.e., the β1 chain of integrins (CD29) and the isoforms of the leukocyte common antigen CD45RA and CD45RO. Methods: Frozen liver samples from 38 patients with advanced ALD were examined immunohistochemically by the avidin-biotin-peroxidase complex method using monoclonal antibodies. Nine patients with alcoholic fatty liver and six patients with only hepatitis C infection were included as controls and studied in a similar manner. Results: CD29 was strongly expressed on the cell membrane of hepatocytes in 18/20 patients with cirrhosis, in 17/18 with alcoholic hepatitis without cirrhosis, in 5/9 of fatty liver and in all six with hepatitis C. CD45RA was present in 5/18 cases of alcoholic hepatitis and in 3/20 cases of cirrhosis on the hepatocytes plasma membrane, in none of the controls, and rarely on lymphocytes. CD45RO was expressed on the surface of lymphocytes in 14/18 cases of alcoholic hepatitis, 13/20 patients with cirrhosis, in 5/9 of fatty liver and in one with hepatitis C. The CD45RO lymphocytes were predominantly CD8 positive. Conclusions: Our results indicate that in ALD the hepatocytes exhibit on their plasma membrane an enhanced expression of the β1 chain of the integrins and less commonly CD45RA. Furthermore, the expression of only CD45RO on the surface of lymphocytes favors the postulate that the intrahepatic lymphocytes in ALD are most likely of the 'memory' type. The results of this study lend further support to the contention that a cell- cell contact precedes a cell-mediated mechanism in the pathogenesis of ALD.

AB - Objectives: In the active stages of alcoholic liver disease (ALD), we have shown previously an enhanced expression of either CD4 or CD8 molecules on CD3+T cells. Class I and II MHC molecules expression were enhanced on lymphocytes, and on the cell membrane of hepatocytes, thus suggesting that an intrahepatic antigen-dependent lymphocyte-hepatocyte interaction occurs. Here we report the pattern of expression of several additional molecules known to participate in nonspecific cell-cell adhesion preceding alloantigen recognition i.e., the β1 chain of integrins (CD29) and the isoforms of the leukocyte common antigen CD45RA and CD45RO. Methods: Frozen liver samples from 38 patients with advanced ALD were examined immunohistochemically by the avidin-biotin-peroxidase complex method using monoclonal antibodies. Nine patients with alcoholic fatty liver and six patients with only hepatitis C infection were included as controls and studied in a similar manner. Results: CD29 was strongly expressed on the cell membrane of hepatocytes in 18/20 patients with cirrhosis, in 17/18 with alcoholic hepatitis without cirrhosis, in 5/9 of fatty liver and in all six with hepatitis C. CD45RA was present in 5/18 cases of alcoholic hepatitis and in 3/20 cases of cirrhosis on the hepatocytes plasma membrane, in none of the controls, and rarely on lymphocytes. CD45RO was expressed on the surface of lymphocytes in 14/18 cases of alcoholic hepatitis, 13/20 patients with cirrhosis, in 5/9 of fatty liver and in one with hepatitis C. The CD45RO lymphocytes were predominantly CD8 positive. Conclusions: Our results indicate that in ALD the hepatocytes exhibit on their plasma membrane an enhanced expression of the β1 chain of the integrins and less commonly CD45RA. Furthermore, the expression of only CD45RO on the surface of lymphocytes favors the postulate that the intrahepatic lymphocytes in ALD are most likely of the 'memory' type. The results of this study lend further support to the contention that a cell- cell contact precedes a cell-mediated mechanism in the pathogenesis of ALD.

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