Expression of S.S.E.A.-1 in the developing dorsal spinal cord of the rat

Martin Oudega, E. Marani, R. W T M Thomeer

Research output: Contribution to journalArticle

Abstract

The trisaccharide 3 (α) fucosyl N-acetyl lactosamine (FAL, also known as stage-specific embryonic antigen; S.S.E.A.-1) is implicated as a regulating cell surface structure in developmental processes such as neuronal recognition and adhesion. An immunocytochemical study, using a monoclonal antibody directed against S.S.E.A.-1 (3B9 or CLB gr/2: see Tetteroo et al., 1984), was carried out on the developing (dorsal) spinal cord of the rat. In the embryonic period FAL was located in the ectoderm (at E10) and in the ventricular zone of the matrix layer (at E11), suggesting an involvement of S.S.E.A.-1 with proliferating, premigratory cells of the rat spinal cord. Between E12 and E16, S.S.E.A.-1 could be discerned in the dorsal aspect of the matrix layer. In addition, FAL was located in the dorsal aspect of the lateral funiculus; the dorsal root entrance zone, at E14. This localization implies a restriction of S.S.E.A.-1 for developing dorsal horn neurons (see Nornes and Das, 1974) as well as for ingrowing primary afferents. From E18 on, FAL could be discerned in the dorsal mantle layer. First, at E18, as three vaguely bounded 'areas' in the developing dorsal horn. Later on, S.S.E.A.-1 could be found in the ventral aspect of the substantia gelatinosa. This localization persisted in the mature spinal cord. S.S.E.A.-1 seems to be involved in the establishment and maintenance of a subset of primary afferents (Dodd and Jessel, 1986).

Original languageEnglish (US)
Pages (from-to)241
Number of pages1
JournalActa Morphologica Neerlando-Scandinavica
Volume27
Issue number3-4
StatePublished - 1989
Externally publishedYes

Fingerprint

CD15 Antigens
Spinal Cord
Stage-Specific Embryonic Antigens
Substantia Gelatinosa
Posterior Horn Cells
Trisaccharides
Ectoderm
Spinal Nerve Roots
Monoclonal Antibodies
Maintenance

ASJC Scopus subject areas

  • Anatomy

Cite this

Expression of S.S.E.A.-1 in the developing dorsal spinal cord of the rat. / Oudega, Martin; Marani, E.; Thomeer, R. W T M.

In: Acta Morphologica Neerlando-Scandinavica, Vol. 27, No. 3-4, 1989, p. 241.

Research output: Contribution to journalArticle

@article{f6300567626d4829b614242f6f5364c1,
title = "Expression of S.S.E.A.-1 in the developing dorsal spinal cord of the rat",
abstract = "The trisaccharide 3 (α) fucosyl N-acetyl lactosamine (FAL, also known as stage-specific embryonic antigen; S.S.E.A.-1) is implicated as a regulating cell surface structure in developmental processes such as neuronal recognition and adhesion. An immunocytochemical study, using a monoclonal antibody directed against S.S.E.A.-1 (3B9 or CLB gr/2: see Tetteroo et al., 1984), was carried out on the developing (dorsal) spinal cord of the rat. In the embryonic period FAL was located in the ectoderm (at E10) and in the ventricular zone of the matrix layer (at E11), suggesting an involvement of S.S.E.A.-1 with proliferating, premigratory cells of the rat spinal cord. Between E12 and E16, S.S.E.A.-1 could be discerned in the dorsal aspect of the matrix layer. In addition, FAL was located in the dorsal aspect of the lateral funiculus; the dorsal root entrance zone, at E14. This localization implies a restriction of S.S.E.A.-1 for developing dorsal horn neurons (see Nornes and Das, 1974) as well as for ingrowing primary afferents. From E18 on, FAL could be discerned in the dorsal mantle layer. First, at E18, as three vaguely bounded 'areas' in the developing dorsal horn. Later on, S.S.E.A.-1 could be found in the ventral aspect of the substantia gelatinosa. This localization persisted in the mature spinal cord. S.S.E.A.-1 seems to be involved in the establishment and maintenance of a subset of primary afferents (Dodd and Jessel, 1986).",
author = "Martin Oudega and E. Marani and Thomeer, {R. W T M}",
year = "1989",
language = "English (US)",
volume = "27",
pages = "241",
journal = "Acta Morphologica Neerlando-Scandinavica",
issn = "0001-6225",
publisher = "Swets & Zeitlinger",
number = "3-4",

}

TY - JOUR

T1 - Expression of S.S.E.A.-1 in the developing dorsal spinal cord of the rat

AU - Oudega, Martin

AU - Marani, E.

AU - Thomeer, R. W T M

PY - 1989

Y1 - 1989

N2 - The trisaccharide 3 (α) fucosyl N-acetyl lactosamine (FAL, also known as stage-specific embryonic antigen; S.S.E.A.-1) is implicated as a regulating cell surface structure in developmental processes such as neuronal recognition and adhesion. An immunocytochemical study, using a monoclonal antibody directed against S.S.E.A.-1 (3B9 or CLB gr/2: see Tetteroo et al., 1984), was carried out on the developing (dorsal) spinal cord of the rat. In the embryonic period FAL was located in the ectoderm (at E10) and in the ventricular zone of the matrix layer (at E11), suggesting an involvement of S.S.E.A.-1 with proliferating, premigratory cells of the rat spinal cord. Between E12 and E16, S.S.E.A.-1 could be discerned in the dorsal aspect of the matrix layer. In addition, FAL was located in the dorsal aspect of the lateral funiculus; the dorsal root entrance zone, at E14. This localization implies a restriction of S.S.E.A.-1 for developing dorsal horn neurons (see Nornes and Das, 1974) as well as for ingrowing primary afferents. From E18 on, FAL could be discerned in the dorsal mantle layer. First, at E18, as three vaguely bounded 'areas' in the developing dorsal horn. Later on, S.S.E.A.-1 could be found in the ventral aspect of the substantia gelatinosa. This localization persisted in the mature spinal cord. S.S.E.A.-1 seems to be involved in the establishment and maintenance of a subset of primary afferents (Dodd and Jessel, 1986).

AB - The trisaccharide 3 (α) fucosyl N-acetyl lactosamine (FAL, also known as stage-specific embryonic antigen; S.S.E.A.-1) is implicated as a regulating cell surface structure in developmental processes such as neuronal recognition and adhesion. An immunocytochemical study, using a monoclonal antibody directed against S.S.E.A.-1 (3B9 or CLB gr/2: see Tetteroo et al., 1984), was carried out on the developing (dorsal) spinal cord of the rat. In the embryonic period FAL was located in the ectoderm (at E10) and in the ventricular zone of the matrix layer (at E11), suggesting an involvement of S.S.E.A.-1 with proliferating, premigratory cells of the rat spinal cord. Between E12 and E16, S.S.E.A.-1 could be discerned in the dorsal aspect of the matrix layer. In addition, FAL was located in the dorsal aspect of the lateral funiculus; the dorsal root entrance zone, at E14. This localization implies a restriction of S.S.E.A.-1 for developing dorsal horn neurons (see Nornes and Das, 1974) as well as for ingrowing primary afferents. From E18 on, FAL could be discerned in the dorsal mantle layer. First, at E18, as three vaguely bounded 'areas' in the developing dorsal horn. Later on, S.S.E.A.-1 could be found in the ventral aspect of the substantia gelatinosa. This localization persisted in the mature spinal cord. S.S.E.A.-1 seems to be involved in the establishment and maintenance of a subset of primary afferents (Dodd and Jessel, 1986).

UR - http://www.scopus.com/inward/record.url?scp=0024814620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024814620&partnerID=8YFLogxK

M3 - Article

VL - 27

SP - 241

JO - Acta Morphologica Neerlando-Scandinavica

JF - Acta Morphologica Neerlando-Scandinavica

SN - 0001-6225

IS - 3-4

ER -