Expression of receptor tyrosine phosphatases during development of the retinotectal projection of the chick

Matthias M. Ledig, Iain W. McKinnell, Thomas Mrsic-Flogel, Jun Wang, Caroline Alvares, Ivor Mason, John Bixby, Bernhard K. Mueller, Andrew W. Stoker

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Receptor tyrosine kinases and receptor protein tyrosine phosphatases (RPTPs) appear to coordinate many aspects of neural development, including axon growth and guidance. Here, we focus on the possible roles of RPTPs in the developing avian retinotectal system. Using both in situ hybridization analysis and immunohistochemistry, we show for the first time that five RPTP genes - CRYPα, CRYP-2, PTPμ, PTPγ, and PTPα - have different but overlapping expression patterns throughout the retina and the tectum. PTPα is restricted to Muller gila cells and radial gila of the tectum, indicating a possible function in controlling neuronal migration. PTPγ expression is restricted to amacrine neurons. CRYPα and CRYP-2 mRNAs in contrast are expressed throughout the retinal ganglion cell layer from where axons grow out to their tectal targets. PTPμ is expressed in a subset of these ganglion cells. CRYPα, CRYP-2, and PTPμ proteins are also localized in growth cones of retinal ganglion cell axons and are present in defined laminae of the tectum. Thus, the spatial and temporal expression of three distinct RPTP subtypes - CRYPα, CRYP-2, and PTPμ - are consistent with the possibility of their involvement in axon growth and guidance of the retinotectal projection.

Original languageEnglish
Pages (from-to)81-96
Number of pages16
JournalJournal of Neurobiology
Volume39
Issue number1
DOIs
StatePublished - Apr 13 1999

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Protein Tyrosine Phosphatases
Phosphoric Monoester Hydrolases
Retinal Ganglion Cells
Axons
Ependymoglial Cells
Growth Cones
Receptor Protein-Tyrosine Kinases
Growth
Ganglia
In Situ Hybridization
Retina
Immunohistochemistry
Neurons
Messenger RNA
tyrosine receptor
Genes
Proteins
Axon Guidance

Keywords

  • Axon guidance
  • Chicken
  • Optic tectum
  • Receptor protein tyrosine phosphatase
  • Retinal ganglion cells
  • Retinotectal projection

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of receptor tyrosine phosphatases during development of the retinotectal projection of the chick. / Ledig, Matthias M.; McKinnell, Iain W.; Mrsic-Flogel, Thomas; Wang, Jun; Alvares, Caroline; Mason, Ivor; Bixby, John; Mueller, Bernhard K.; Stoker, Andrew W.

In: Journal of Neurobiology, Vol. 39, No. 1, 13.04.1999, p. 81-96.

Research output: Contribution to journalArticle

Ledig, Matthias M. ; McKinnell, Iain W. ; Mrsic-Flogel, Thomas ; Wang, Jun ; Alvares, Caroline ; Mason, Ivor ; Bixby, John ; Mueller, Bernhard K. ; Stoker, Andrew W. / Expression of receptor tyrosine phosphatases during development of the retinotectal projection of the chick. In: Journal of Neurobiology. 1999 ; Vol. 39, No. 1. pp. 81-96.
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