Expression of rat thyrotropin-releasing hormone (TRH) Gene in TRH-Producing Tissues of Transgenic Mice Requires Sequences Located in Exon 1

Wayne E Balkan, Marie A. Tavianini, Peter J. Gkonos, Bernard A. Roos

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

TRH, an amidated tripeptide secreted by certain hypothalamic neurons, is a principal regulator of TSH secretion and thyroid hormone release. TRH is also produced by other neurons in the central nervous system, where it appears to function as a neuromodulator or neurotransmitter, and by certain endocrine cells, where it may act as an autocrine or paracrine factor. The genomic organization of the rat TRH (rTRH) gene is well understood; however, the domains of the rTRH gene that regulate expression are less well characterized. We observed that the region between -47 and +6 of the rTRH gene (relative to the transcription start site at +1) was active in CA-77 cells, a medullary thyroid carcinoma cell line model of TRH production, but was not active in transgenic mice. Inclusion of most of exon 1 (84 out of 103 bp; -47 to+ 84) increased promoter activity in CA-77 cells and was active in transgenic mice, principally in tissues that normally express the TRH gene. Further lengthening of the 5' end to -243, -547, or -776 retained this expression in TRH-producing tissues in transgenic mice, while further increasing activity in CA-77 cells. These results suggest that cis element(s) located within exon 1 are necessary for the expression of the rTRH gene in vivo.

Original languageEnglish (US)
Pages (from-to)252-259
Number of pages8
JournalEndocrinology
Volume139
Issue number1
DOIs
StatePublished - 1998
Externally publishedYes

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Thyrotropin-Releasing Hormone
Transgenic Mice
Exons
Genes
Neurotransmitter Agents
Neurons
Endocrine Cells
Transcription Initiation Site
Thyroid Hormones
Central Nervous System
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Endocrinology

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Expression of rat thyrotropin-releasing hormone (TRH) Gene in TRH-Producing Tissues of Transgenic Mice Requires Sequences Located in Exon 1. / Balkan, Wayne E; Tavianini, Marie A.; Gkonos, Peter J.; Roos, Bernard A.

In: Endocrinology, Vol. 139, No. 1, 1998, p. 252-259.

Research output: Contribution to journalArticle

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