Expression of PTPRO in the interneurons of adult mouse olfactory bulb

Takenori Kotani, Yoji Murata, Hiroshi Ohnishi, Munemasa Mori, Shinya Kusakari, Yasuyuki Saito, Hideki Okazawa, John Bixby, Takashi Matozaki

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8 Scopus citations

Abstract

PTPRO is a receptor-type protein tyrosine phosphatase (PTP) with a single catalytic domain in its cytoplasmic region and multiple fibronectin type III-like domains in its extracellular region. In the chick, PTPRO mRNA has been shown to be particularly abundant in embryonic brain, and PTPRO is implicated in axon growth and guidance during embryonic development. However, the temporal and spatial expression of PTPRO protein in the mammalian CNS, particularly in the juvenile and adult mammalian brain, has not been evaluated in any detail. By immunohistofluorescence analysis with a monoclonal antibody to PTPRO, we show that PTPRO is widely expressed throughout the mouse brain from embryonic day 16 to postnatal day 1, while expression is largely confined to the olfactory bulb (OB) and olfactory tubercle in the adult brain. In the OB, PTPRO protein is expressed predominantly in the external plexiform layer, the granule cell layer, and the glomerular layer (GL). In these regions, expression of PTPRO is predominant in interneurons such as γ-aminobutyric acid (GABA)-ergic or calretinin (CR)-positive granule cells. In addition, PTPRO is expressed in GABAergic, CR-positive, tyrosine hydroxylase-positive, or neurocalcin-positive periglomerular cells in the GL. Costaining of PTPRO with other neuronal markers suggests that PTPRO is likely to be localized to the dendrites or dendritic spines of these olfactory interneurons. Thus, PTPRO might participate in regulation of dendritic morphology or synapse formation of interneurons in the adult mouse OB.

Original languageEnglish
Pages (from-to)119-136
Number of pages18
JournalJournal of Comparative Neurology
Volume518
Issue number2
DOIs
StatePublished - Jan 15 2010

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Keywords

  • CNS
  • Granule cells
  • Localization
  • Maturation
  • Periglomerular cells
  • Receptor-type tyrosine phosphatase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Kotani, T., Murata, Y., Ohnishi, H., Mori, M., Kusakari, S., Saito, Y., Okazawa, H., Bixby, J., & Matozaki, T. (2010). Expression of PTPRO in the interneurons of adult mouse olfactory bulb. Journal of Comparative Neurology, 518(2), 119-136. https://doi.org/10.1002/cne.22239