Expression of PTPRO during mouse development suggests involvement in axonogenesis and differentiation of NT-3 and NGF-dependent neurons

Pedro J. Beltran, John Bixby, Brian A. Masters

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Competition and cooperation between type II and type III receptor protein tyrosine phosphatases (RPTPs) regulate axon extension and pathfinding in Drosophila. The first step to investigate whether RPTPs influence axon growth in the more complex vertebrate nervous system is to identify which neurons express a particular RPTP. We studied the expression of mouse PTPRO, a type III RPTP with an extracellular region containing eight fibronectin type III domains, during embryogenesis and after birth. Mouse PTPRO mRNA is expressed exclusively in two cell types: neurons and kidney podocytes. Maximal expression in the brain was coincident with mid to late gestation and axonogenesis in the brain. We cloned two cDNAs, including a splice variant without sequence coding of 28 amino acids within the juxtamembrane domain that was found mostly in kidney. In situ hybridization detected mPTPRO mRNA in the cerebral cortex, olfactory bulb and nucleus, hippocampus, motor neurons, and the spinal cord midline. In addition, mPTPRO mRNA was found throughout dorsal root, cranial, and sympathetic ganglia and within kidney glomeruli. Mouse PTPRO mRNA was observed in neuron populations expressing TrkA, the high-affinity nerve growth factor receptor, or TrkC, the neurotrophin-3 receptor, and immunoreactive mPTPRO and TrkC colocalized in large dorsal root ganglia proprioceptive neurons. Our results suggest that mPTPRO is involved in the differentiation and axonogenesis of central and peripheral nervous system neurons, where it is in a position to modulate intracellular responses to neurotrophin-3 and/or nerve growth factor.

Original languageEnglish
Pages (from-to)384-395
Number of pages12
JournalJournal of Comparative Neurology
Volume456
Issue number4
DOIs
StatePublished - Feb 17 2003

Fingerprint

Nerve Growth Factor
Protein Tyrosine Phosphatases
Neurons
Messenger RNA
Spinal Ganglia
trkC Receptor
Kidney Glomerulus
Neurotrophin 3
Kidney
Nerve Growth Factor Receptor
Podocytes
Sympathetic Ganglia
Olfactory Bulb
Peripheral Nervous System
Brain
Motor Neurons
Cerebral Cortex
Nervous System
Drosophila
Embryonic Development

Keywords

  • Dorsal root ganglia
  • Juxtamembrane domain
  • Protein tyrosine phosphatases

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of PTPRO during mouse development suggests involvement in axonogenesis and differentiation of NT-3 and NGF-dependent neurons. / Beltran, Pedro J.; Bixby, John; Masters, Brian A.

In: Journal of Comparative Neurology, Vol. 456, No. 4, 17.02.2003, p. 384-395.

Research output: Contribution to journalArticle

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