Expression of progenitor markers is associated with the functionality of a bioartificial adrenal cortex

Mariya Balyura, Evgeny Gelfgat, Charlotte Steenblock, Andreas Androutsellis-Theotokis, Gerard Ruiz-Babot, Leonardo Guasti, Martin Werdermann, Barbara Ludwig, Tobias Bornstein, Andrew V Schally, Ana Brennand, Stefan R. Bornstein

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Encapsulation of primary bovine adrenocortical cells in alginate is an efficacious model of a bioartificial adrenal cortex. Such a bioartificial adrenal cortex can be used for the restoration of lost adrenal function in vivo as well as for in vitro modeling of the adrenal microenvironment and for investigation of cell–cell interactions in the adrenals. The aim of this work was the optimization of a bioartificial adrenal cortex, that is the generation of a highly productive, self-regenerating, long-term functioning and immune tolerant bioartificial organ. To achieve this, it is necessary that adrenocortical stem and progenitor cells are present in the bioartificial gland, as these undifferentiated cells play important roles in the function of the mature gland. Here, we verified the presence of adrenocortical progenitors in cultures of bovine adrenocortical cells, studied the dynamics of their appearance and growth and determined the optimal time point for cell encapsulation. These procedures increased the functional life span and reduced the immunogenicity of the bioartificial adrenal cortex. This model allows the use of the luteinizing hormone-releasing hormone (LHRH) agonist triptorelin, the neuropeptide bombesin, and retinoic acid to alter cell number and the release of cortisol over long periods of time.

Original languageEnglish (US)
Article numbere0194643
JournalPLoS One
Volume13
Issue number3
DOIs
StatePublished - Mar 1 2018

Fingerprint

adrenal cortex
Adrenal Cortex
Encapsulation
Triptorelin Pamoate
Bombesin
Tretinoin
Neuropeptides
Gonadotropin-Releasing Hormone
Restoration
Bioartificial Organs
Hydrocortisone
encapsulation
Stem Cells
cells
stem cells
bombesin
hormone agonists
retinoic acid
cattle
neuropeptides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Balyura, M., Gelfgat, E., Steenblock, C., Androutsellis-Theotokis, A., Ruiz-Babot, G., Guasti, L., ... Bornstein, S. R. (2018). Expression of progenitor markers is associated with the functionality of a bioartificial adrenal cortex. PLoS One, 13(3), [e0194643]. https://doi.org/10.1371/journal.pone.0194643

Expression of progenitor markers is associated with the functionality of a bioartificial adrenal cortex. / Balyura, Mariya; Gelfgat, Evgeny; Steenblock, Charlotte; Androutsellis-Theotokis, Andreas; Ruiz-Babot, Gerard; Guasti, Leonardo; Werdermann, Martin; Ludwig, Barbara; Bornstein, Tobias; Schally, Andrew V; Brennand, Ana; Bornstein, Stefan R.

In: PLoS One, Vol. 13, No. 3, e0194643, 01.03.2018.

Research output: Contribution to journalArticle

Balyura, M, Gelfgat, E, Steenblock, C, Androutsellis-Theotokis, A, Ruiz-Babot, G, Guasti, L, Werdermann, M, Ludwig, B, Bornstein, T, Schally, AV, Brennand, A & Bornstein, SR 2018, 'Expression of progenitor markers is associated with the functionality of a bioartificial adrenal cortex', PLoS One, vol. 13, no. 3, e0194643. https://doi.org/10.1371/journal.pone.0194643
Balyura M, Gelfgat E, Steenblock C, Androutsellis-Theotokis A, Ruiz-Babot G, Guasti L et al. Expression of progenitor markers is associated with the functionality of a bioartificial adrenal cortex. PLoS One. 2018 Mar 1;13(3). e0194643. https://doi.org/10.1371/journal.pone.0194643
Balyura, Mariya ; Gelfgat, Evgeny ; Steenblock, Charlotte ; Androutsellis-Theotokis, Andreas ; Ruiz-Babot, Gerard ; Guasti, Leonardo ; Werdermann, Martin ; Ludwig, Barbara ; Bornstein, Tobias ; Schally, Andrew V ; Brennand, Ana ; Bornstein, Stefan R. / Expression of progenitor markers is associated with the functionality of a bioartificial adrenal cortex. In: PLoS One. 2018 ; Vol. 13, No. 3.
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