Expression of otd orthologs in the amphipod crustacean, Parhyale hawaiensis

William Browne, Bernhard G M Schmid, Ernst A. Wimmer, Mark Q. Martindale

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The arthropod head is a complex metameric structure. In insects, orthodenticle (otd) functions as a 'head gap gene' and plays a significant role in patterning and development of the anterior head ectoderm, the protocerebrum, and the ventral midline. In this study, we characterize the structure and developmental deployment of two otd paralogs in the amphipod crustacean, Parhyale hawaiensis. Photd1is initially expressed at gastrulation through germband stages in a bilaterally symmetric, restricted region of the anterior head ectoderm and also in a single column of cells along the ventral midline. Late in embryogenesis, Photd1is expressed within the developing anterior brain and the expression along the embryonic midline has become restricted to a stereotypic group of segmentally reiterated cells. The second ortholog Photd2, however, has a unique temporal-spatial expression pattern and is not detected until after the head lobes have been organized in the developing ectoderm of the germband during late germband stages. Anteriorly, Photd2 is coincident with the Photd1head expression domain; however, Photd2 is not detected along the ventral midline during formation of the germband and only appears in the ventral midline late in embryonic development in a restricted group of cells distinct from those expressing Photd1. The early expression of Photd1in the anterior head ectoderm is consistent with a role as a head gap gene. The more posterior expression of Photd1is suggestive of a role in patterning the embryonic ventral midline. Photd2 expression appears too late to play a role in early head patterning but may contribute to latter patterning in restricted regions of both the head and the ventral midline. The comparative analysis of otd reveals the divergence of gene expression and gene function associated with duplication of this important developmental gene.

Original languageEnglish
Pages (from-to)581-595
Number of pages15
JournalDevelopment Genes and Evolution
Volume216
Issue number10
DOIs
StatePublished - Oct 1 2006
Externally publishedYes

Fingerprint

Parhyale hawaiensis
Amphipoda
amphipod
crustacean
Ectoderm
Crustacea
Head
gene
embryogenesis
genes
brain
Embryonic Development
embryonic development
cells
arthropod
Genes
gene expression
Developmental Genes
arthropods
Gastrulation

Keywords

  • Amphipod
  • Brain development
  • CNS
  • Crustacean
  • Head development
  • Midline
  • Neurogenesis
  • Orthodenticle
  • Parhyale

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Expression of otd orthologs in the amphipod crustacean, Parhyale hawaiensis. / Browne, William; Schmid, Bernhard G M; Wimmer, Ernst A.; Martindale, Mark Q.

In: Development Genes and Evolution, Vol. 216, No. 10, 01.10.2006, p. 581-595.

Research output: Contribution to journalArticle

Browne, William ; Schmid, Bernhard G M ; Wimmer, Ernst A. ; Martindale, Mark Q. / Expression of otd orthologs in the amphipod crustacean, Parhyale hawaiensis. In: Development Genes and Evolution. 2006 ; Vol. 216, No. 10. pp. 581-595.
@article{e3a34487ee8f401d848fcdf9d48c15e4,
title = "Expression of otd orthologs in the amphipod crustacean, Parhyale hawaiensis",
abstract = "The arthropod head is a complex metameric structure. In insects, orthodenticle (otd) functions as a 'head gap gene' and plays a significant role in patterning and development of the anterior head ectoderm, the protocerebrum, and the ventral midline. In this study, we characterize the structure and developmental deployment of two otd paralogs in the amphipod crustacean, Parhyale hawaiensis. Photd1is initially expressed at gastrulation through germband stages in a bilaterally symmetric, restricted region of the anterior head ectoderm and also in a single column of cells along the ventral midline. Late in embryogenesis, Photd1is expressed within the developing anterior brain and the expression along the embryonic midline has become restricted to a stereotypic group of segmentally reiterated cells. The second ortholog Photd2, however, has a unique temporal-spatial expression pattern and is not detected until after the head lobes have been organized in the developing ectoderm of the germband during late germband stages. Anteriorly, Photd2 is coincident with the Photd1head expression domain; however, Photd2 is not detected along the ventral midline during formation of the germband and only appears in the ventral midline late in embryonic development in a restricted group of cells distinct from those expressing Photd1. The early expression of Photd1in the anterior head ectoderm is consistent with a role as a head gap gene. The more posterior expression of Photd1is suggestive of a role in patterning the embryonic ventral midline. Photd2 expression appears too late to play a role in early head patterning but may contribute to latter patterning in restricted regions of both the head and the ventral midline. The comparative analysis of otd reveals the divergence of gene expression and gene function associated with duplication of this important developmental gene.",
keywords = "Amphipod, Brain development, CNS, Crustacean, Head development, Midline, Neurogenesis, Orthodenticle, Parhyale",
author = "William Browne and Schmid, {Bernhard G M} and Wimmer, {Ernst A.} and Martindale, {Mark Q.}",
year = "2006",
month = "10",
day = "1",
doi = "10.1007/s00427-006-0074-7",
language = "English",
volume = "216",
pages = "581--595",
journal = "Development Genes and Evolution",
issn = "0949-944X",
publisher = "Springer Verlag",
number = "10",

}

TY - JOUR

T1 - Expression of otd orthologs in the amphipod crustacean, Parhyale hawaiensis

AU - Browne, William

AU - Schmid, Bernhard G M

AU - Wimmer, Ernst A.

AU - Martindale, Mark Q.

PY - 2006/10/1

Y1 - 2006/10/1

N2 - The arthropod head is a complex metameric structure. In insects, orthodenticle (otd) functions as a 'head gap gene' and plays a significant role in patterning and development of the anterior head ectoderm, the protocerebrum, and the ventral midline. In this study, we characterize the structure and developmental deployment of two otd paralogs in the amphipod crustacean, Parhyale hawaiensis. Photd1is initially expressed at gastrulation through germband stages in a bilaterally symmetric, restricted region of the anterior head ectoderm and also in a single column of cells along the ventral midline. Late in embryogenesis, Photd1is expressed within the developing anterior brain and the expression along the embryonic midline has become restricted to a stereotypic group of segmentally reiterated cells. The second ortholog Photd2, however, has a unique temporal-spatial expression pattern and is not detected until after the head lobes have been organized in the developing ectoderm of the germband during late germband stages. Anteriorly, Photd2 is coincident with the Photd1head expression domain; however, Photd2 is not detected along the ventral midline during formation of the germband and only appears in the ventral midline late in embryonic development in a restricted group of cells distinct from those expressing Photd1. The early expression of Photd1in the anterior head ectoderm is consistent with a role as a head gap gene. The more posterior expression of Photd1is suggestive of a role in patterning the embryonic ventral midline. Photd2 expression appears too late to play a role in early head patterning but may contribute to latter patterning in restricted regions of both the head and the ventral midline. The comparative analysis of otd reveals the divergence of gene expression and gene function associated with duplication of this important developmental gene.

AB - The arthropod head is a complex metameric structure. In insects, orthodenticle (otd) functions as a 'head gap gene' and plays a significant role in patterning and development of the anterior head ectoderm, the protocerebrum, and the ventral midline. In this study, we characterize the structure and developmental deployment of two otd paralogs in the amphipod crustacean, Parhyale hawaiensis. Photd1is initially expressed at gastrulation through germband stages in a bilaterally symmetric, restricted region of the anterior head ectoderm and also in a single column of cells along the ventral midline. Late in embryogenesis, Photd1is expressed within the developing anterior brain and the expression along the embryonic midline has become restricted to a stereotypic group of segmentally reiterated cells. The second ortholog Photd2, however, has a unique temporal-spatial expression pattern and is not detected until after the head lobes have been organized in the developing ectoderm of the germband during late germband stages. Anteriorly, Photd2 is coincident with the Photd1head expression domain; however, Photd2 is not detected along the ventral midline during formation of the germband and only appears in the ventral midline late in embryonic development in a restricted group of cells distinct from those expressing Photd1. The early expression of Photd1in the anterior head ectoderm is consistent with a role as a head gap gene. The more posterior expression of Photd1is suggestive of a role in patterning the embryonic ventral midline. Photd2 expression appears too late to play a role in early head patterning but may contribute to latter patterning in restricted regions of both the head and the ventral midline. The comparative analysis of otd reveals the divergence of gene expression and gene function associated with duplication of this important developmental gene.

KW - Amphipod

KW - Brain development

KW - CNS

KW - Crustacean

KW - Head development

KW - Midline

KW - Neurogenesis

KW - Orthodenticle

KW - Parhyale

UR - http://www.scopus.com/inward/record.url?scp=33749080817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749080817&partnerID=8YFLogxK

U2 - 10.1007/s00427-006-0074-7

DO - 10.1007/s00427-006-0074-7

M3 - Article

VL - 216

SP - 581

EP - 595

JO - Development Genes and Evolution

JF - Development Genes and Evolution

SN - 0949-944X

IS - 10

ER -