Expression of neuronal-NOS in developing basal forebrain cholinergic neurons

Regulation by NGF

David M. Holtzman, Sandra Lee, Yiwen Li, Jane Chua-Couzens, Houhui Xia, David S. Bredt, William C. Mobley

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Nerve growth factor (NGF) acts through the receptor tyrosine kinase trkA to serve as a trophic factor for cholinergic neurons in the medial septal nucleus and vertical limb of the diagonal band. We have previously shown that the neuronal isoform of nitric oxide synthase (NOS) is selectively expressed in a large fraction of trkA-expressing cholinergic neurons in these brain regions in the adult rat, and that NGF induces the expression of neuronal- NOS in these cells. Herein, we show that: 1) neuronal-NOS is also localized to these neurons in the developing septum; 2) the expression of neuronal-NOS is regulated in the developing medial septal nucleus and vertical limb of the diagonal band; 3) neuronal-NOS regulation parallels that for other markers of basal forebrain cholinergic neuron differentiation, such as cholineacetyltransferase; and 4) NGF infusion in the postnatal period induces robust increases in neuronal-NOS mRNA and in NOS activity in the basal forebrain. Taken together with earlier findings, our results suggest that neuronal-NOS has a role in the differentiation and mature function of septal cholinergic neurons. Through enhancing neuronal-NOS synthesis, endogenous NGF is likely to regulate NO functions in vivo.

Original languageEnglish
Pages (from-to)861-868
Number of pages8
JournalNeurochemical Research
Volume21
Issue number7
StatePublished - Jul 1 1996
Externally publishedYes

Fingerprint

Nitric Oxide Synthase Type I
Cholinergic Neurons
Nerve Growth Factor
Cholinergic Agents
Neurons
Septal Nuclei
Nitric Oxide Synthase
Extremities
Receptor Protein-Tyrosine Kinases
Basal Forebrain
Rats
Brain
Protein Isoforms
Messenger RNA

Keywords

  • basal forebrain cholinergic neurons
  • n-NOS
  • NGF
  • trkA

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Holtzman, D. M., Lee, S., Li, Y., Chua-Couzens, J., Xia, H., Bredt, D. S., & Mobley, W. C. (1996). Expression of neuronal-NOS in developing basal forebrain cholinergic neurons: Regulation by NGF. Neurochemical Research, 21(7), 861-868.

Expression of neuronal-NOS in developing basal forebrain cholinergic neurons : Regulation by NGF. / Holtzman, David M.; Lee, Sandra; Li, Yiwen; Chua-Couzens, Jane; Xia, Houhui; Bredt, David S.; Mobley, William C.

In: Neurochemical Research, Vol. 21, No. 7, 01.07.1996, p. 861-868.

Research output: Contribution to journalArticle

Holtzman, DM, Lee, S, Li, Y, Chua-Couzens, J, Xia, H, Bredt, DS & Mobley, WC 1996, 'Expression of neuronal-NOS in developing basal forebrain cholinergic neurons: Regulation by NGF', Neurochemical Research, vol. 21, no. 7, pp. 861-868.
Holtzman DM, Lee S, Li Y, Chua-Couzens J, Xia H, Bredt DS et al. Expression of neuronal-NOS in developing basal forebrain cholinergic neurons: Regulation by NGF. Neurochemical Research. 1996 Jul 1;21(7):861-868.
Holtzman, David M. ; Lee, Sandra ; Li, Yiwen ; Chua-Couzens, Jane ; Xia, Houhui ; Bredt, David S. ; Mobley, William C. / Expression of neuronal-NOS in developing basal forebrain cholinergic neurons : Regulation by NGF. In: Neurochemical Research. 1996 ; Vol. 21, No. 7. pp. 861-868.
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