Expression of n-acetylglucosaminyltransferase V mRNA in mammalian tissues and cell lines

G. S. Perug, M. Shoreibah, I. Margitich, M. Pierce, Nevis L. Fregien

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The expression of N-acetylglucosaminyltransferase V (Glc-NAc-T V) is increased in many oncogenically transformed cells and in cell lines which are induced to proliferate. In order to characterize the regulation of GlcNAc-T V at the molecular level, we have examined the expression of Glc-NAc-T V mRNAs in a number of mouse tissues and in several human and rodent cell lines. The Glc-NAc-T V mRNA is expressed in different amounts in the various mouse tissues, with the greatest amount observed in brain, followed by thymus, kidney, lung, intestine, heart and stomach, and no transcripts detected in liver or skeletal muscle. Measurements of Glc-NAc-T V enzymatic activity, by contrast, show brain to have lower levels of activity than several of the other tissues, suggesting possible post-translational regulation. We find that there are two Glc-NAc-T V transcripts in most of the RNAs analysed. The rodent cell lines all express both a 7.5 and a 9.5 kb mRNA. with the smaller transcript being more abundant. The human cells have mRNAs of 4.5 and 9.5 kb. Both mRNAs are expressed in HepG2 and MCF-7 cells, while A431 cells express only the 4.5 kb mRNA and HL60 cells express only the 9.5 kb transcript. Furthermore, only the 9.5 kb mRNA appears to be increased, along with activity, when HepG2 cells are stimulated to proliferate, suggesting that the two mRNAs may be under different regulatory controls. Also, a Glc-NAc-T V-deficient, mutant lymphoma cell line, PHAR2.1, was found to express mRNAs which are larger than the normal mRNAs, possibly due to an insertion or aberrant splicing, resulting in a defective mRNA.

Original languageEnglish
Pages (from-to)867-871
Number of pages5
JournalGlycobiology
Volume4
Issue number6
StatePublished - Dec 1 1994

Fingerprint

Messenger RNA
Cells
Tissue
Cell Line
Line
Cell
Express
alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase
Mouse
Rodentia
Brain
Cell line
Thymus
Skeletal muscle
HL-60 Cells
MCF-7 Cells
Hep G2 Cells
Kidney
Lung
RNA

Keywords

  • Gene expression
  • Glycosyltransferase
  • mRNA

ASJC Scopus subject areas

  • Biochemistry
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Hematology

Cite this

Perug, G. S., Shoreibah, M., Margitich, I., Pierce, M., & Fregien, N. L. (1994). Expression of n-acetylglucosaminyltransferase V mRNA in mammalian tissues and cell lines. Glycobiology, 4(6), 867-871.

Expression of n-acetylglucosaminyltransferase V mRNA in mammalian tissues and cell lines. / Perug, G. S.; Shoreibah, M.; Margitich, I.; Pierce, M.; Fregien, Nevis L.

In: Glycobiology, Vol. 4, No. 6, 01.12.1994, p. 867-871.

Research output: Contribution to journalArticle

Perug, GS, Shoreibah, M, Margitich, I, Pierce, M & Fregien, NL 1994, 'Expression of n-acetylglucosaminyltransferase V mRNA in mammalian tissues and cell lines', Glycobiology, vol. 4, no. 6, pp. 867-871.
Perug, G. S. ; Shoreibah, M. ; Margitich, I. ; Pierce, M. ; Fregien, Nevis L. / Expression of n-acetylglucosaminyltransferase V mRNA in mammalian tissues and cell lines. In: Glycobiology. 1994 ; Vol. 4, No. 6. pp. 867-871.
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