Expression of MUC2 and MUC4 proteins and cytokines: Early markers of intestinal graft rejection

Nir Wasserberg, Shashikumar K. Salgar, Dinghua Yang, Phillip Ruiz, Samuel B. Ho, Werviston L. De Faria, Sergio F Santiago, Carlos E. Gandia, Joshua Miller, Yaron Niv, Andreas G. Tzakis

Research output: Contribution to journalArticle

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Abstract

Background. Histopathologic examination (HP) is the primary method of monitoring intestinal graft rejection. Alterations in mucin levels have been demonstrated in bowel diseases. The aim of this study was to detect early markers of intestinal graft rejection based on mucin and cytokine levels. Methods. Allogeneic and syngeneic orthotopic intestinal transplantations were performed in untreated Lewis strain recipient rats from Dark Agouti and Lewis strain donors, respectively (unmodified rejection and nonrejection groups). Similarly, allogeneic and syngeneic orthotopic intestinal transplantations were performed in tacrolimus (immunosuppression)-treated groups. HP was performed on hematoxylineosin and periodic acid Schiff-stained sections. Expression of MUC2 and MUC4 proteins and of mRNA was detected by immunohistochemistry and Northern analysis, respectively. Interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and transforming growth factor-β1 were measured by reverse transcription-polymerase chain reaction. Results. HP revealed early or mild rejection on day 3, moderate rejection on day 5, and severe rejection on day 7 posttransplantation (posttx) in the unmodified rejection group. A significant (P<0.01) increase in MUC2 and MUC4 expression was observed on day 3 posttx in the allogeneic rejection group compared with syngeneic controls; the levels decreased by day 7. Goblet cells were significantly more frequent on day 3 compared with days 5 and 7 posttx (P<0.01). IFN-γ and TNF-α expression were also higher in the rejection group. Conclusions. Early transplant rejection is associated with increased MUC2, MUC4, IFN-γ, and TNF-α expression. These markers combined with HP may assist in the diagnosis of early intestinal graft rejection.

Original languageEnglish
Pages (from-to)1249-1255
Number of pages7
JournalTransplantation
Volume75
Issue number8
DOIs
StatePublished - Apr 27 2003

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Graft Rejection
Interferons
Cytokines
Tumor Necrosis Factor-alpha
Mucins
Proteins
Transplantation
Periodic Acid
Goblet Cells
Transforming Growth Factors
Tacrolimus
Immunosuppression
Reverse Transcription
Immunohistochemistry
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Wasserberg, N., Salgar, S. K., Yang, D., Ruiz, P., Ho, S. B., De Faria, W. L., ... Tzakis, A. G. (2003). Expression of MUC2 and MUC4 proteins and cytokines: Early markers of intestinal graft rejection. Transplantation, 75(8), 1249-1255. https://doi.org/10.1097/01.TP.0000060566.34001.CA

Expression of MUC2 and MUC4 proteins and cytokines : Early markers of intestinal graft rejection. / Wasserberg, Nir; Salgar, Shashikumar K.; Yang, Dinghua; Ruiz, Phillip; Ho, Samuel B.; De Faria, Werviston L.; Santiago, Sergio F; Gandia, Carlos E.; Miller, Joshua; Niv, Yaron; Tzakis, Andreas G.

In: Transplantation, Vol. 75, No. 8, 27.04.2003, p. 1249-1255.

Research output: Contribution to journalArticle

Wasserberg, N, Salgar, SK, Yang, D, Ruiz, P, Ho, SB, De Faria, WL, Santiago, SF, Gandia, CE, Miller, J, Niv, Y & Tzakis, AG 2003, 'Expression of MUC2 and MUC4 proteins and cytokines: Early markers of intestinal graft rejection', Transplantation, vol. 75, no. 8, pp. 1249-1255. https://doi.org/10.1097/01.TP.0000060566.34001.CA
Wasserberg, Nir ; Salgar, Shashikumar K. ; Yang, Dinghua ; Ruiz, Phillip ; Ho, Samuel B. ; De Faria, Werviston L. ; Santiago, Sergio F ; Gandia, Carlos E. ; Miller, Joshua ; Niv, Yaron ; Tzakis, Andreas G. / Expression of MUC2 and MUC4 proteins and cytokines : Early markers of intestinal graft rejection. In: Transplantation. 2003 ; Vol. 75, No. 8. pp. 1249-1255.
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abstract = "Background. Histopathologic examination (HP) is the primary method of monitoring intestinal graft rejection. Alterations in mucin levels have been demonstrated in bowel diseases. The aim of this study was to detect early markers of intestinal graft rejection based on mucin and cytokine levels. Methods. Allogeneic and syngeneic orthotopic intestinal transplantations were performed in untreated Lewis strain recipient rats from Dark Agouti and Lewis strain donors, respectively (unmodified rejection and nonrejection groups). Similarly, allogeneic and syngeneic orthotopic intestinal transplantations were performed in tacrolimus (immunosuppression)-treated groups. HP was performed on hematoxylineosin and periodic acid Schiff-stained sections. Expression of MUC2 and MUC4 proteins and of mRNA was detected by immunohistochemistry and Northern analysis, respectively. Interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and transforming growth factor-β1 were measured by reverse transcription-polymerase chain reaction. Results. HP revealed early or mild rejection on day 3, moderate rejection on day 5, and severe rejection on day 7 posttransplantation (posttx) in the unmodified rejection group. A significant (P<0.01) increase in MUC2 and MUC4 expression was observed on day 3 posttx in the allogeneic rejection group compared with syngeneic controls; the levels decreased by day 7. Goblet cells were significantly more frequent on day 3 compared with days 5 and 7 posttx (P<0.01). IFN-γ and TNF-α expression were also higher in the rejection group. Conclusions. Early transplant rejection is associated with increased MUC2, MUC4, IFN-γ, and TNF-α expression. These markers combined with HP may assist in the diagnosis of early intestinal graft rejection.",
author = "Nir Wasserberg and Salgar, {Shashikumar K.} and Dinghua Yang and Phillip Ruiz and Ho, {Samuel B.} and {De Faria}, {Werviston L.} and Santiago, {Sergio F} and Gandia, {Carlos E.} and Joshua Miller and Yaron Niv and Tzakis, {Andreas G.}",
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T1 - Expression of MUC2 and MUC4 proteins and cytokines

T2 - Early markers of intestinal graft rejection

AU - Wasserberg, Nir

AU - Salgar, Shashikumar K.

AU - Yang, Dinghua

AU - Ruiz, Phillip

AU - Ho, Samuel B.

AU - De Faria, Werviston L.

AU - Santiago, Sergio F

AU - Gandia, Carlos E.

AU - Miller, Joshua

AU - Niv, Yaron

AU - Tzakis, Andreas G.

PY - 2003/4/27

Y1 - 2003/4/27

N2 - Background. Histopathologic examination (HP) is the primary method of monitoring intestinal graft rejection. Alterations in mucin levels have been demonstrated in bowel diseases. The aim of this study was to detect early markers of intestinal graft rejection based on mucin and cytokine levels. Methods. Allogeneic and syngeneic orthotopic intestinal transplantations were performed in untreated Lewis strain recipient rats from Dark Agouti and Lewis strain donors, respectively (unmodified rejection and nonrejection groups). Similarly, allogeneic and syngeneic orthotopic intestinal transplantations were performed in tacrolimus (immunosuppression)-treated groups. HP was performed on hematoxylineosin and periodic acid Schiff-stained sections. Expression of MUC2 and MUC4 proteins and of mRNA was detected by immunohistochemistry and Northern analysis, respectively. Interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and transforming growth factor-β1 were measured by reverse transcription-polymerase chain reaction. Results. HP revealed early or mild rejection on day 3, moderate rejection on day 5, and severe rejection on day 7 posttransplantation (posttx) in the unmodified rejection group. A significant (P<0.01) increase in MUC2 and MUC4 expression was observed on day 3 posttx in the allogeneic rejection group compared with syngeneic controls; the levels decreased by day 7. Goblet cells were significantly more frequent on day 3 compared with days 5 and 7 posttx (P<0.01). IFN-γ and TNF-α expression were also higher in the rejection group. Conclusions. Early transplant rejection is associated with increased MUC2, MUC4, IFN-γ, and TNF-α expression. These markers combined with HP may assist in the diagnosis of early intestinal graft rejection.

AB - Background. Histopathologic examination (HP) is the primary method of monitoring intestinal graft rejection. Alterations in mucin levels have been demonstrated in bowel diseases. The aim of this study was to detect early markers of intestinal graft rejection based on mucin and cytokine levels. Methods. Allogeneic and syngeneic orthotopic intestinal transplantations were performed in untreated Lewis strain recipient rats from Dark Agouti and Lewis strain donors, respectively (unmodified rejection and nonrejection groups). Similarly, allogeneic and syngeneic orthotopic intestinal transplantations were performed in tacrolimus (immunosuppression)-treated groups. HP was performed on hematoxylineosin and periodic acid Schiff-stained sections. Expression of MUC2 and MUC4 proteins and of mRNA was detected by immunohistochemistry and Northern analysis, respectively. Interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and transforming growth factor-β1 were measured by reverse transcription-polymerase chain reaction. Results. HP revealed early or mild rejection on day 3, moderate rejection on day 5, and severe rejection on day 7 posttransplantation (posttx) in the unmodified rejection group. A significant (P<0.01) increase in MUC2 and MUC4 expression was observed on day 3 posttx in the allogeneic rejection group compared with syngeneic controls; the levels decreased by day 7. Goblet cells were significantly more frequent on day 3 compared with days 5 and 7 posttx (P<0.01). IFN-γ and TNF-α expression were also higher in the rejection group. Conclusions. Early transplant rejection is associated with increased MUC2, MUC4, IFN-γ, and TNF-α expression. These markers combined with HP may assist in the diagnosis of early intestinal graft rejection.

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