Expression of mRNA for human type-I LHRH receptor transcript forms in human benign prostatic hyperplasia

Bernadett Rózsa, Aliz Juhász, Andrea Treszl, György Tóth, Tibor Flaskó, Balázs Dezsö, Norman L. Block, Andrew V. Schally, Gábor Halmos

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The presence of four different isoforms of luteinizing hormone-releasing hormones (LHRH) and one LHRH receptor (LHRH-R) has been reported in vertebrates. In the human genome only LHRH-I and LHRH-II genes have been identified. The human LHRH-I gene is composed of four exons separated by three introns. Three LHRH receptor or receptor-like genes have been demonstrated. The well-established type-I LHRH receptor (LHRH-R-I) gene is composed of three exons separated by two introns. In this study we investigated the expression of transcript forms of LHRH-R-I in human benign prostatic hyperplasia (BPH) with reverse transcriptase-polymerase chain reaction (RT-PCR) using gene specific primers. Thirty-five human BPH specimens were obtained at surgery. Normal human pituitaries collected at autopsy served as control. RNA extraction and RT-PCR with gene-specific primers for LHRH-R-I forward (F1)/reverse (R1), LHRH-R-I F2/R3, LHRH-R-I F1′/R2′ were carried out to determine the mRNA expression for LHRH-R-I transcript forms. The expected PCR products amplified with gene specific primers were LHRH-R-I F1/R1 with 319 bp, LHRHR-I F2/R3 with 309 bp and LHRH-R-I F1′/R2′ with 219 bp. PCR products for LHRH-R-I F1/R1 were detected in 21 (60%) and for LHRH-R-I F2/R3 in 5 of 35 (14%) BPH samples. No PCR products for LHRH-R-I F1′/R2′ were found. In conclusion, we detected mRNA for LHRH-R-I in human BPH specimens. Our results suggest that LHRH-R-I gene may have more than two splice variants or uncharacterised transcript forms of LHRH-R-I. Our findings support the merit of further investigation of the expression of LHRH-R-I and its transcript forms in human BPH.

Original languageEnglish (US)
Pages (from-to)1053-1059
Number of pages7
JournalInternational journal of oncology
Issue number5
StatePublished - Nov 1 2009


  • Human benign prostatic hyperplasia
  • Receptors for LHRH
  • Transcript forms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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