Expression of MALT1 oncogene in hematopoietic stem/progenitor cells recapitulates the pathogenesis of human lymphoma in mice

Carolina Vicente-Dueñas, Lorena Fontán, Ines Gonzalez-Herrero, Isabel Romero-Camarero, Victor Segura, M. Angela Aznar, Esther Alonso-Escudero, Elena Campos-Sanchez, Lucía Ruiz-Roca, Marcos Barajas-Diego, Ainara Sagardoy, Jose I. Martinez-Ferrandis, Fernando Abollo-Jimenez, Cristina Bertolo, Ivan Peñuelas, Francisco J. Garcia-Criado, María B. García-Cenador, Thomas Tousseyn, Xabier Agirre, Felipe Prosper & 15 others Federico Garcia-Bragado, Ellen D. McPhail, Izidore Lossos, Ming Qing Du, Teresa Flores, Jesus M. Hernandez-Rivas, Marcos Gonzalez, Antonio Salar, Beatriz Bellosillo, Eulogio Conde, Reiner Siebert, Xavier Sagaert, Cesar Cobaleda, Isidro Sanchez-Garcia, Jose A. Martinez-Climent

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Chromosomal translocations involving the MALT1 gene are hallmarks of mucosa-associated lymphoid tissue (MALT) lymphoma. To date, targeting these translocations to mouse B cells has failed to reproduce human disease. Here, we induced MALT1 expression in mouse Sca1+Lin- hematopoietic stem/progenitor cells, which showed NF-κB activation and early lymphoid priming, being selectively skewed toward B-cell differentiation. These cells accumulated in extranodal tissues and gave rise to clonal tumors recapitulating the principal clinical, biological, and molecular genetic features of MALT lymphoma. Deletion of p53 gene accelerated tumor onset and induced transformation of MALT lymphoma to activated B-cell diffuse large-cell lymphoma (ABC-DLBCL). Treatment of MALT1-induced lymphomas with a specific inhibitor of MALT1 proteolytic activity decreased cell viability, indicating that endogenous Malt1 signaling was required for tumor cell survival. Our study shows that human-like lymphomas can be modeled in mice by targeting MALT1 expression to hematopoietic stem/progenitor cells, demonstrating the oncogenic role of MALT1 in lymphomagenesis. Furthermore, this work establishes a molecular link between MALT lymphoma and ABC-DLBCL, and provides mouse models to test MALT1 inhibitors. Finally, our results suggest that hematopoietic stem/progenitor cells may be involved in the pathogenesis of human mature B-cell lymphomas.

Original languageEnglish
Pages (from-to)10534-10539
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number26
DOIs
StatePublished - Jun 26 2012

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Hematopoietic Stem Cells
Oncogenes
Lymphoma
Marginal Zone B-Cell Lymphoma
B-Lymphocytes
Cell Survival
Neoplasms
Genetic Translocation
Lymphoma, Large B-Cell, Diffuse
p53 Genes
B-Cell Lymphoma
Molecular Biology
Cell Differentiation
Genes

Keywords

  • Cancer stem cells
  • Molecular-directed therapy
  • Transgenic mice
  • Tumor reprogramming

ASJC Scopus subject areas

  • General

Cite this

Vicente-Dueñas, C., Fontán, L., Gonzalez-Herrero, I., Romero-Camarero, I., Segura, V., Aznar, M. A., ... Martinez-Climent, J. A. (2012). Expression of MALT1 oncogene in hematopoietic stem/progenitor cells recapitulates the pathogenesis of human lymphoma in mice. Proceedings of the National Academy of Sciences of the United States of America, 109(26), 10534-10539. https://doi.org/10.1073/pnas.1204127109

Expression of MALT1 oncogene in hematopoietic stem/progenitor cells recapitulates the pathogenesis of human lymphoma in mice. / Vicente-Dueñas, Carolina; Fontán, Lorena; Gonzalez-Herrero, Ines; Romero-Camarero, Isabel; Segura, Victor; Aznar, M. Angela; Alonso-Escudero, Esther; Campos-Sanchez, Elena; Ruiz-Roca, Lucía; Barajas-Diego, Marcos; Sagardoy, Ainara; Martinez-Ferrandis, Jose I.; Abollo-Jimenez, Fernando; Bertolo, Cristina; Peñuelas, Ivan; Garcia-Criado, Francisco J.; García-Cenador, María B.; Tousseyn, Thomas; Agirre, Xabier; Prosper, Felipe; Garcia-Bragado, Federico; McPhail, Ellen D.; Lossos, Izidore; Du, Ming Qing; Flores, Teresa; Hernandez-Rivas, Jesus M.; Gonzalez, Marcos; Salar, Antonio; Bellosillo, Beatriz; Conde, Eulogio; Siebert, Reiner; Sagaert, Xavier; Cobaleda, Cesar; Sanchez-Garcia, Isidro; Martinez-Climent, Jose A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 26, 26.06.2012, p. 10534-10539.

Research output: Contribution to journalArticle

Vicente-Dueñas, C, Fontán, L, Gonzalez-Herrero, I, Romero-Camarero, I, Segura, V, Aznar, MA, Alonso-Escudero, E, Campos-Sanchez, E, Ruiz-Roca, L, Barajas-Diego, M, Sagardoy, A, Martinez-Ferrandis, JI, Abollo-Jimenez, F, Bertolo, C, Peñuelas, I, Garcia-Criado, FJ, García-Cenador, MB, Tousseyn, T, Agirre, X, Prosper, F, Garcia-Bragado, F, McPhail, ED, Lossos, I, Du, MQ, Flores, T, Hernandez-Rivas, JM, Gonzalez, M, Salar, A, Bellosillo, B, Conde, E, Siebert, R, Sagaert, X, Cobaleda, C, Sanchez-Garcia, I & Martinez-Climent, JA 2012, 'Expression of MALT1 oncogene in hematopoietic stem/progenitor cells recapitulates the pathogenesis of human lymphoma in mice', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 26, pp. 10534-10539. https://doi.org/10.1073/pnas.1204127109
Vicente-Dueñas, Carolina ; Fontán, Lorena ; Gonzalez-Herrero, Ines ; Romero-Camarero, Isabel ; Segura, Victor ; Aznar, M. Angela ; Alonso-Escudero, Esther ; Campos-Sanchez, Elena ; Ruiz-Roca, Lucía ; Barajas-Diego, Marcos ; Sagardoy, Ainara ; Martinez-Ferrandis, Jose I. ; Abollo-Jimenez, Fernando ; Bertolo, Cristina ; Peñuelas, Ivan ; Garcia-Criado, Francisco J. ; García-Cenador, María B. ; Tousseyn, Thomas ; Agirre, Xabier ; Prosper, Felipe ; Garcia-Bragado, Federico ; McPhail, Ellen D. ; Lossos, Izidore ; Du, Ming Qing ; Flores, Teresa ; Hernandez-Rivas, Jesus M. ; Gonzalez, Marcos ; Salar, Antonio ; Bellosillo, Beatriz ; Conde, Eulogio ; Siebert, Reiner ; Sagaert, Xavier ; Cobaleda, Cesar ; Sanchez-Garcia, Isidro ; Martinez-Climent, Jose A. / Expression of MALT1 oncogene in hematopoietic stem/progenitor cells recapitulates the pathogenesis of human lymphoma in mice. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 26. pp. 10534-10539.
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abstract = "Chromosomal translocations involving the MALT1 gene are hallmarks of mucosa-associated lymphoid tissue (MALT) lymphoma. To date, targeting these translocations to mouse B cells has failed to reproduce human disease. Here, we induced MALT1 expression in mouse Sca1+Lin- hematopoietic stem/progenitor cells, which showed NF-κB activation and early lymphoid priming, being selectively skewed toward B-cell differentiation. These cells accumulated in extranodal tissues and gave rise to clonal tumors recapitulating the principal clinical, biological, and molecular genetic features of MALT lymphoma. Deletion of p53 gene accelerated tumor onset and induced transformation of MALT lymphoma to activated B-cell diffuse large-cell lymphoma (ABC-DLBCL). Treatment of MALT1-induced lymphomas with a specific inhibitor of MALT1 proteolytic activity decreased cell viability, indicating that endogenous Malt1 signaling was required for tumor cell survival. Our study shows that human-like lymphomas can be modeled in mice by targeting MALT1 expression to hematopoietic stem/progenitor cells, demonstrating the oncogenic role of MALT1 in lymphomagenesis. Furthermore, this work establishes a molecular link between MALT lymphoma and ABC-DLBCL, and provides mouse models to test MALT1 inhibitors. Finally, our results suggest that hematopoietic stem/progenitor cells may be involved in the pathogenesis of human mature B-cell lymphomas.",
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AU - Romero-Camarero, Isabel

AU - Segura, Victor

AU - Aznar, M. Angela

AU - Alonso-Escudero, Esther

AU - Campos-Sanchez, Elena

AU - Ruiz-Roca, Lucía

AU - Barajas-Diego, Marcos

AU - Sagardoy, Ainara

AU - Martinez-Ferrandis, Jose I.

AU - Abollo-Jimenez, Fernando

AU - Bertolo, Cristina

AU - Peñuelas, Ivan

AU - Garcia-Criado, Francisco J.

AU - García-Cenador, María B.

AU - Tousseyn, Thomas

AU - Agirre, Xabier

AU - Prosper, Felipe

AU - Garcia-Bragado, Federico

AU - McPhail, Ellen D.

AU - Lossos, Izidore

AU - Du, Ming Qing

AU - Flores, Teresa

AU - Hernandez-Rivas, Jesus M.

AU - Gonzalez, Marcos

AU - Salar, Antonio

AU - Bellosillo, Beatriz

AU - Conde, Eulogio

AU - Siebert, Reiner

AU - Sagaert, Xavier

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