Expression of Ly-6A/E alloantigens in thymocyte and T-lymphocyte subsets: variability related to the Ly-6a and Ly-6b haplotypes

Elaine K. Codias, Carolyn Cray, Ruben D. Baler, Robert B Levy, Thomas Malek

Research output: Contribution to journalArticle

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Abstract

We have studied the cellular basis for differential expression of the Ly-6A/E alloantigen on T cells obtained from mice of the Ly-6a (10-20% Ly-6A/E+) and Ly-6b (50-60% Ly-6A/E+) haplotypes. During T-cell ontogeny only a small fraction (< 12 %) of thymocytes expressed Ly-6A/E. By 4 weeks of age adult levels of Ly-6A/E bearing lymphocytes were seen in peripheral lymphoid tissue. Immunohistochemical studies of the thymus revealed that Ly-6A/E+ cells were located predominantly in the medulla with small clusters of Ly-6A/E+ cells throughout the cortex. Consistent with this result, phenotypic studies showed that in the adult thymus the majority of Ly-6A/E expression was on mature CD4+ CD8- and CD4- CD8+ cortisone-resistant and precursor CD4- CD8- thymocytes. However, a much higher percentage of CD4+ CD8- and CD4- CD8- thymocytes as well as CD4+ CD8- peripheral T cells expressed Ly-6A/E from Ly-6b mice. Furthermore, although gamma interferon induced increased Ly-6A/E expression in certain thymocyte and T-cell subsets, this induction functioned preferentially for cells obtained from Ly-6b mice. Studies using F1 hybrid mice (Ly-6a × Ly-6b) indicated that the "basal" level of Ly-6A/E expression on these subsets appeared to be under codominant genetic control, whereas gamma interferon-induced regulation of Ly-6A/E expression appeared to be under dominant genetic control. Collectively, these results suggest that the expression of Ly-6A/E on a particular T-cell subset is established in the thymus and is a stable characteristic of each haplotype. In addition, the low levels of Ly-6A/E expression for the Ly-6a haplotype appear to be partially due to the inability of the majority of resting CD4+ T cells to express Ly-6A/E and to the relatively poor induction of this protein by gamma interferon.

Original languageEnglish
Pages (from-to)98-107
Number of pages10
JournalImmunogenetics
Volume29
Issue number2
DOIs
StatePublished - Feb 1 1989

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Isoantigens
T-Lymphocyte Subsets
Thymocytes
Haplotypes
Thymus Gland
Interferon-gamma
T-Lymphocytes
Cortisone
Lymphoid Tissue
Lymphocytes
Proteins

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Expression of Ly-6A/E alloantigens in thymocyte and T-lymphocyte subsets : variability related to the Ly-6a and Ly-6b haplotypes. / Codias, Elaine K.; Cray, Carolyn; Baler, Ruben D.; Levy, Robert B; Malek, Thomas.

In: Immunogenetics, Vol. 29, No. 2, 01.02.1989, p. 98-107.

Research output: Contribution to journalArticle

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abstract = "We have studied the cellular basis for differential expression of the Ly-6A/E alloantigen on T cells obtained from mice of the Ly-6a (10-20{\%} Ly-6A/E+) and Ly-6b (50-60{\%} Ly-6A/E+) haplotypes. During T-cell ontogeny only a small fraction (< 12 {\%}) of thymocytes expressed Ly-6A/E. By 4 weeks of age adult levels of Ly-6A/E bearing lymphocytes were seen in peripheral lymphoid tissue. Immunohistochemical studies of the thymus revealed that Ly-6A/E+ cells were located predominantly in the medulla with small clusters of Ly-6A/E+ cells throughout the cortex. Consistent with this result, phenotypic studies showed that in the adult thymus the majority of Ly-6A/E expression was on mature CD4+ CD8- and CD4- CD8+ cortisone-resistant and precursor CD4- CD8- thymocytes. However, a much higher percentage of CD4+ CD8- and CD4- CD8- thymocytes as well as CD4+ CD8- peripheral T cells expressed Ly-6A/E from Ly-6b mice. Furthermore, although gamma interferon induced increased Ly-6A/E expression in certain thymocyte and T-cell subsets, this induction functioned preferentially for cells obtained from Ly-6b mice. Studies using F1 hybrid mice (Ly-6a × Ly-6b) indicated that the {"}basal{"} level of Ly-6A/E expression on these subsets appeared to be under codominant genetic control, whereas gamma interferon-induced regulation of Ly-6A/E expression appeared to be under dominant genetic control. Collectively, these results suggest that the expression of Ly-6A/E on a particular T-cell subset is established in the thymus and is a stable characteristic of each haplotype. In addition, the low levels of Ly-6A/E expression for the Ly-6a haplotype appear to be partially due to the inability of the majority of resting CD4+ T cells to express Ly-6A/E and to the relatively poor induction of this protein by gamma interferon.",
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