Abstract
We have studied the cellular basis for differential expression of the Ly-6A/E alloantigen on T cells obtained from mice of the Ly-6a (10-20% Ly-6A/E+) and Ly-6b (50-60% Ly-6A/E+) haplotypes. During T-cell ontogeny only a small fraction (< 12 %) of thymocytes expressed Ly-6A/E. By 4 weeks of age adult levels of Ly-6A/E bearing lymphocytes were seen in peripheral lymphoid tissue. Immunohistochemical studies of the thymus revealed that Ly-6A/E+ cells were located predominantly in the medulla with small clusters of Ly-6A/E+ cells throughout the cortex. Consistent with this result, phenotypic studies showed that in the adult thymus the majority of Ly-6A/E expression was on mature CD4+ CD8- and CD4- CD8+ cortisone-resistant and precursor CD4- CD8- thymocytes. However, a much higher percentage of CD4+ CD8- and CD4- CD8- thymocytes as well as CD4+ CD8- peripheral T cells expressed Ly-6A/E from Ly-6b mice. Furthermore, although gamma interferon induced increased Ly-6A/E expression in certain thymocyte and T-cell subsets, this induction functioned preferentially for cells obtained from Ly-6b mice. Studies using F1 hybrid mice (Ly-6a × Ly-6b) indicated that the "basal" level of Ly-6A/E expression on these subsets appeared to be under codominant genetic control, whereas gamma interferon-induced regulation of Ly-6A/E expression appeared to be under dominant genetic control. Collectively, these results suggest that the expression of Ly-6A/E on a particular T-cell subset is established in the thymus and is a stable characteristic of each haplotype. In addition, the low levels of Ly-6A/E expression for the Ly-6a haplotype appear to be partially due to the inability of the majority of resting CD4+ T cells to express Ly-6A/E and to the relatively poor induction of this protein by gamma interferon.
| Original language | English |
|---|---|
| Pages (from-to) | 98-107 |
| Number of pages | 10 |
| Journal | Immunogenetics |
| Volume | 29 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 1989 |
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ASJC Scopus subject areas
- Immunology
- Genetics
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Expression of Ly-6A/E alloantigens in thymocyte and T-lymphocyte subsets : variability related to the Ly-6a and Ly-6b haplotypes. / Codias, Elaine K.; Cray, Carolyn; Baler, Ruben D.; Levy, Robert B; Malek, Thomas.
In: Immunogenetics, Vol. 29, No. 2, 01.02.1989, p. 98-107.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of Ly-6A/E alloantigens in thymocyte and T-lymphocyte subsets
T2 - variability related to the Ly-6a and Ly-6b haplotypes
AU - Codias, Elaine K.
AU - Cray, Carolyn
AU - Baler, Ruben D.
AU - Levy, Robert B
AU - Malek, Thomas
PY - 1989/2/1
Y1 - 1989/2/1
N2 - We have studied the cellular basis for differential expression of the Ly-6A/E alloantigen on T cells obtained from mice of the Ly-6a (10-20% Ly-6A/E+) and Ly-6b (50-60% Ly-6A/E+) haplotypes. During T-cell ontogeny only a small fraction (< 12 %) of thymocytes expressed Ly-6A/E. By 4 weeks of age adult levels of Ly-6A/E bearing lymphocytes were seen in peripheral lymphoid tissue. Immunohistochemical studies of the thymus revealed that Ly-6A/E+ cells were located predominantly in the medulla with small clusters of Ly-6A/E+ cells throughout the cortex. Consistent with this result, phenotypic studies showed that in the adult thymus the majority of Ly-6A/E expression was on mature CD4+ CD8- and CD4- CD8+ cortisone-resistant and precursor CD4- CD8- thymocytes. However, a much higher percentage of CD4+ CD8- and CD4- CD8- thymocytes as well as CD4+ CD8- peripheral T cells expressed Ly-6A/E from Ly-6b mice. Furthermore, although gamma interferon induced increased Ly-6A/E expression in certain thymocyte and T-cell subsets, this induction functioned preferentially for cells obtained from Ly-6b mice. Studies using F1 hybrid mice (Ly-6a × Ly-6b) indicated that the "basal" level of Ly-6A/E expression on these subsets appeared to be under codominant genetic control, whereas gamma interferon-induced regulation of Ly-6A/E expression appeared to be under dominant genetic control. Collectively, these results suggest that the expression of Ly-6A/E on a particular T-cell subset is established in the thymus and is a stable characteristic of each haplotype. In addition, the low levels of Ly-6A/E expression for the Ly-6a haplotype appear to be partially due to the inability of the majority of resting CD4+ T cells to express Ly-6A/E and to the relatively poor induction of this protein by gamma interferon.
AB - We have studied the cellular basis for differential expression of the Ly-6A/E alloantigen on T cells obtained from mice of the Ly-6a (10-20% Ly-6A/E+) and Ly-6b (50-60% Ly-6A/E+) haplotypes. During T-cell ontogeny only a small fraction (< 12 %) of thymocytes expressed Ly-6A/E. By 4 weeks of age adult levels of Ly-6A/E bearing lymphocytes were seen in peripheral lymphoid tissue. Immunohistochemical studies of the thymus revealed that Ly-6A/E+ cells were located predominantly in the medulla with small clusters of Ly-6A/E+ cells throughout the cortex. Consistent with this result, phenotypic studies showed that in the adult thymus the majority of Ly-6A/E expression was on mature CD4+ CD8- and CD4- CD8+ cortisone-resistant and precursor CD4- CD8- thymocytes. However, a much higher percentage of CD4+ CD8- and CD4- CD8- thymocytes as well as CD4+ CD8- peripheral T cells expressed Ly-6A/E from Ly-6b mice. Furthermore, although gamma interferon induced increased Ly-6A/E expression in certain thymocyte and T-cell subsets, this induction functioned preferentially for cells obtained from Ly-6b mice. Studies using F1 hybrid mice (Ly-6a × Ly-6b) indicated that the "basal" level of Ly-6A/E expression on these subsets appeared to be under codominant genetic control, whereas gamma interferon-induced regulation of Ly-6A/E expression appeared to be under dominant genetic control. Collectively, these results suggest that the expression of Ly-6A/E on a particular T-cell subset is established in the thymus and is a stable characteristic of each haplotype. In addition, the low levels of Ly-6A/E expression for the Ly-6a haplotype appear to be partially due to the inability of the majority of resting CD4+ T cells to express Ly-6A/E and to the relatively poor induction of this protein by gamma interferon.
UR - http://www.scopus.com/inward/record.url?scp=0024552010&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024552010&partnerID=8YFLogxK
U2 - 10.1007/BF00395857
DO - 10.1007/BF00395857
M3 - Article
C2 - 2492483
AN - SCOPUS:0024552010
VL - 29
SP - 98
EP - 107
JO - Immunogenetics
JF - Immunogenetics
SN - 0093-7711
IS - 2
ER -