Expression of interfering RNAs from an HIV-1 Tat-inducible chimeric promoter

Viraj R. Sanghvi, Laura F. Steel

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The therapeutic value of antiviral interfering RNAs could be improved by technologies that limit their expression to the infected cell population. The HIV-1 Tat-inducible viral LTR and LTR-containing chimeric promoters have previously been used to drive expression of antiviral RNAs and proteins directed against HIV-1. Here, we characterize an alternative promoter, consisting of a chicken β-actin core promoter fused to the viral TAR element, for the conditional expression of interfering RNAs. This promoter, that we refer to as the CK-TAR promoter, can induce levels of silencing comparable to the viral LTR in response to Tat produced from co-transfected plasmids or during viral replication. While the CK-TAR promoter shows a modest level of basal activity, similar to the viral LTR, it is less responsive to the extracellular stimuli tested including LPS, TNFα, and PMA. The CK-TAR promoter is an alternative Tat-inducible promoter with the potential to minimize the risk of vector mobilization and to drive polycistronic expression of interfering RNAs.

Original languageEnglish (US)
Pages (from-to)106-111
Number of pages6
JournalVirus Research
Volume155
Issue number1
DOIs
StatePublished - Jan 2011
Externally publishedYes

Keywords

  • Antiviral
  • HIV-1
  • MicroRNA
  • TAR
  • Tat-inducible

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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