Expression of IL-10, IL-4 and interferon-gamma in unstimulated and mitogen-stimulated peripheral blood lymphocytes from HIV-seropositive patients

F. Diaz-Mitoma, A. Kumar, S. Karimi, M. Kryworuchko, M. P. Daftarian, W. D. Creery, L. G. Filion, W. Cameron

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Infection of immune cells with HIV induces dysregulation of cytokines which may play a vital role in HIV pathogenesis. We analysed the expression of T helper type 1 (Th1) (interferon-gamma (IFN-γ)) and Th2 (IL-4, IL-10) type cytokines in peripheral blood lymphocytes (PBL) from HIV+ patients. The semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that IFN-γ mRNA in unstimulated PBL was significantly decreased and IL-10 mRNA was significantly upregulated in patients with < 400 CD4+ T cells/mm3 (n = 30) as compared to patients with > 400 CD4+ T cells/mm3 (n = 6) and normal controls (n = 16). In addition, IL-10 mRNA levels were inversely associated with IFN-γ expression. Similar results were obtained by measuring IL-10 production in the supernatants of PBL cultured in vitro without stimulation by employing an enzyme immunosorbent assay (ELISA). However, the levels of IL-4 and IFN-γ produced by unstimulated PBL were undetectable by ELISA. Mitogen stimulation of PBL revealed two groups of HIV+ individuals based on IL-10 production. PBL from one set of individuals produced low levels of IL-10 (low IL-10 producers) whereas the other group produced IL-10 comparable to that of normal controls (IL-10 producers). Production of IL-4 was significantly reduced in HIV+ individuals with < 400 CD4+ T cells/mm3 as compared to the normal controls. However, ability to produce IFN-γ by mitogen-stimulated total PBL and CD4+ purified cells was not impaired in HIV+ individuals. These results suggest that unstimulated and mitogen-stimulated PBL of HIV+ individuals exhibit dysregulation of Th2 type cytokines which may play a role in HIV immunopathogenesis.

Original languageEnglish (US)
Pages (from-to)31-39
Number of pages9
JournalClinical and Experimental Immunology
Volume102
Issue number1
StatePublished - Jan 1 1995

Keywords

  • HIV
  • IL-10
  • IL-4
  • Interferon-gamma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Diaz-Mitoma, F., Kumar, A., Karimi, S., Kryworuchko, M., Daftarian, M. P., Creery, W. D., Filion, L. G., & Cameron, W. (1995). Expression of IL-10, IL-4 and interferon-gamma in unstimulated and mitogen-stimulated peripheral blood lymphocytes from HIV-seropositive patients. Clinical and Experimental Immunology, 102(1), 31-39.