Expression of HIV-1 and interleukin-6 in lumbosacral dorsal root ganglia of patients with AIDS

M. Yoshioka, P. Shapshak, A. K. Srivastava, R. V. Stewart, S. J. Nelson, Walter G Bradley, J. R. Berger, R. H. Rhodes, N. C J Sun, S. Nakamura

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Abstract

We examined the immunopathology and the expression of human immunodeficiency virus type 1 (HIV-1) in lumbosacral dorsal root ganglia (DRGs) from 16 patients with acquired immunodeficiency syndrome (AIDS) and 10 HIV-1-seronegative controls. Using in situ hybridization, we detected HIV-1 RNA in a few perivascular cells in DRGs from five of 16 AIDS patients (31%). In addition, using polymerase chain reaction, we detected HIV-1 DNA more frequently in DRGs from four of five AIDS patients (80%) examined. We detected interleukin-6 (IL-6) immunoreactivity in endothelial cells in DRGs from seven of 16 AIDS patients (44%) but from none of 10 HIV-1-seronegative controls (0%). We found more nodules of Nageotte, CD8+ T lymphocytes, and intercellular adhesion molecule-1 (ICAM-1)-positive endothelial cells and mononuclear cells in DRGs from AIDS patients than in DRGs from controls. Increased numbers of nodules of Nageotte in DRGs of AIDS patients were associated with detection of HIV-1 RNA by in situ hybridization and detection of IL-6 by immunohistochemistry. We conclude that low levels of replication of HIV-1 through cytotoxic T lymphocytes or expression of cytokines, may play a role in the subclinical degeneration of sensory neurons frequently observed in DRGs of AIDS patients.

Original languageEnglish
Pages (from-to)1120-1130
Number of pages11
JournalNeurology
Volume44
Issue number6
StatePublished - Jun 1 1994

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Spinal Ganglia
HIV-1
Interleukin-6
Acquired Immunodeficiency Syndrome
In Situ Hybridization
Endothelial Cells
RNA
Cytotoxic T-Lymphocytes
Sensory Receptor Cells
Intercellular Adhesion Molecule-1
Immunohistochemistry
Cytokines
T-Lymphocytes
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Yoshioka, M., Shapshak, P., Srivastava, A. K., Stewart, R. V., Nelson, S. J., Bradley, W. G., ... Nakamura, S. (1994). Expression of HIV-1 and interleukin-6 in lumbosacral dorsal root ganglia of patients with AIDS. Neurology, 44(6), 1120-1130.

Expression of HIV-1 and interleukin-6 in lumbosacral dorsal root ganglia of patients with AIDS. / Yoshioka, M.; Shapshak, P.; Srivastava, A. K.; Stewart, R. V.; Nelson, S. J.; Bradley, Walter G; Berger, J. R.; Rhodes, R. H.; Sun, N. C J; Nakamura, S.

In: Neurology, Vol. 44, No. 6, 01.06.1994, p. 1120-1130.

Research output: Contribution to journalArticle

Yoshioka, M, Shapshak, P, Srivastava, AK, Stewart, RV, Nelson, SJ, Bradley, WG, Berger, JR, Rhodes, RH, Sun, NCJ & Nakamura, S 1994, 'Expression of HIV-1 and interleukin-6 in lumbosacral dorsal root ganglia of patients with AIDS', Neurology, vol. 44, no. 6, pp. 1120-1130.
Yoshioka M, Shapshak P, Srivastava AK, Stewart RV, Nelson SJ, Bradley WG et al. Expression of HIV-1 and interleukin-6 in lumbosacral dorsal root ganglia of patients with AIDS. Neurology. 1994 Jun 1;44(6):1120-1130.
Yoshioka, M. ; Shapshak, P. ; Srivastava, A. K. ; Stewart, R. V. ; Nelson, S. J. ; Bradley, Walter G ; Berger, J. R. ; Rhodes, R. H. ; Sun, N. C J ; Nakamura, S. / Expression of HIV-1 and interleukin-6 in lumbosacral dorsal root ganglia of patients with AIDS. In: Neurology. 1994 ; Vol. 44, No. 6. pp. 1120-1130.
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