Expression of HCLCA1 in cystic fibrosis lungs is associated with mucus overproduction

H. P. Hauber, A. Tsicopoulos, B. Wallaert, S. Griffin, N. G. McElvaney, P. Daigneault, Z. Mueller, R. Olivenstein, K. J. Holroyd, Roy C Levitt, Qutayba Hamid

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Mucus overproduction is typical in cystic fibrosis (CF) airway disease. The human calcium-activated chloride channel, hCLCA1, has been reported to be upregulated by interleukin (IL)-9 and to regulate the expression of mucins. Therefore, the expression of IL-9, IL-9 receptor (IL-9R) and hCLCA1 between the lungs of CF patients and healthy control subjects was compared. Endoscopic biopsy samples of bronchial mucosa from 10 CF patients and six control subjects were stained with periodic acid-Schiff. IL-9, IL-9R and hCLCA1 expression was determined by immunocytochemistry. Expression of hCLCA1 mRNA was also determined by in situ hybridisation. The present study found significant increases in IL-9, IL-9R and hCLCA1 immunoreactivity, hCLCA1 mRNA expression, and numbers of mucus-producing cells in the mucosa of CF patients compared to control subjects. Positive correlations were found between IL-9R-positive-cells with IL-9-positive cells and hCLCA1-positive cells, and between PAS-positive cells with hCLCA1-positive cells and IL-9R-positive cells. Expression of hCLCA1 mRNA was colocalised with IL-9R expression and PAS-positive staining in epithelial cells. Increased expression of interleukin-9 and interleukin-9 receptor, as well as an upregulation of the human calcium-activated chloride channel, hCLCA1, in mucus-producing epithelium of cystic fibrosis patients, support the hypothesis that interleukin-9 contributes to mucus overproduction in cystic fibrosis airway disease.

Original languageEnglish
Pages (from-to)846-850
Number of pages5
JournalEuropean Respiratory Journal
Volume23
Issue number6
StatePublished - Jun 1 2004
Externally publishedYes

Fingerprint

Interleukin-9
Mucus
Cystic Fibrosis
Interleukins
Lung
Interleukin-9 Receptors
Chloride Channels
Messenger RNA
Mucous Membrane
Periodic Acid
Mucins
In Situ Hybridization
Healthy Volunteers
Up-Regulation
Epithelium
Epithelial Cells
Immunohistochemistry
Staining and Labeling
Biopsy

Keywords

  • Cystic fibrosis
  • hCLCA1
  • Interleukin-9
  • Mucus

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Hauber, H. P., Tsicopoulos, A., Wallaert, B., Griffin, S., McElvaney, N. G., Daigneault, P., ... Hamid, Q. (2004). Expression of HCLCA1 in cystic fibrosis lungs is associated with mucus overproduction. European Respiratory Journal, 23(6), 846-850.

Expression of HCLCA1 in cystic fibrosis lungs is associated with mucus overproduction. / Hauber, H. P.; Tsicopoulos, A.; Wallaert, B.; Griffin, S.; McElvaney, N. G.; Daigneault, P.; Mueller, Z.; Olivenstein, R.; Holroyd, K. J.; Levitt, Roy C; Hamid, Qutayba.

In: European Respiratory Journal, Vol. 23, No. 6, 01.06.2004, p. 846-850.

Research output: Contribution to journalArticle

Hauber, HP, Tsicopoulos, A, Wallaert, B, Griffin, S, McElvaney, NG, Daigneault, P, Mueller, Z, Olivenstein, R, Holroyd, KJ, Levitt, RC & Hamid, Q 2004, 'Expression of HCLCA1 in cystic fibrosis lungs is associated with mucus overproduction', European Respiratory Journal, vol. 23, no. 6, pp. 846-850.
Hauber HP, Tsicopoulos A, Wallaert B, Griffin S, McElvaney NG, Daigneault P et al. Expression of HCLCA1 in cystic fibrosis lungs is associated with mucus overproduction. European Respiratory Journal. 2004 Jun 1;23(6):846-850.
Hauber, H. P. ; Tsicopoulos, A. ; Wallaert, B. ; Griffin, S. ; McElvaney, N. G. ; Daigneault, P. ; Mueller, Z. ; Olivenstein, R. ; Holroyd, K. J. ; Levitt, Roy C ; Hamid, Qutayba. / Expression of HCLCA1 in cystic fibrosis lungs is associated with mucus overproduction. In: European Respiratory Journal. 2004 ; Vol. 23, No. 6. pp. 846-850.
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