Expression of growth hormone-releasing hormone (GHRH) and splice variant of GHRH receptors in normal mouse tissues

C. Christodoulou, Andrew V Schally, I. Chatzistamou, A. Kondi-Pafiti, K. Lamnissou, S. Kouloheri, A. Kalofoutis, H. Kiaris

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Growth hormone-releasing hormone (GHRH) stimulates the production and release of growth hormone in the pituitary and induces cell proliferation in a variety of peripheral tissues and tumors. These extrapituitary effects of GHRH are in many cases mediated by a splice variant of GHRH receptor designated SV1 that differs from the pituitary GHRH receptor in a small portion of its amino-terminal region. While SV1 has been detected in several primary tumors and many cancer cell lines its expression in normal tissues remains unclear. In this study we report the results of an immunohistochemical analysis for SV1 and GHRH expression in normal mouse tissues. For the detection of SV1 immunoreactivity we used a polyclonal antiserum against segments 1-25 of the SV1 receptor protein. Mouse heart, colon, lungs, small intestine, stomach and kidneys exhibited increased SV1 immunoreactivity. These tissues were also positive for GHRH expression, however, tissues such as the endometrium were positive only for GHRH and not for SV1 expression. On the contrary, testis were positive for SV1 and not for GHRH expression. These results indicate that SV1 may play a role in normal physiology.

Original languageEnglish
Pages (from-to)105-108
Number of pages4
JournalRegulatory Peptides
Volume136
Issue number1-3
DOIs
StatePublished - Sep 11 2006

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Growth Hormone-Releasing Hormone
Tissue
Pituitary Hormone-Regulating Hormone Receptors
Growth Hormone
Tumors
Neoplasms
Physiology
Cell proliferation
Endometrium
Small Intestine
somatotropin releasing hormone receptor
Testis
Immune Sera
Stomach
Colon
Cells
Cell Proliferation
Kidney
Cell Line
Lung

Keywords

  • Autocrine
  • Extrapituitary
  • GHRH
  • Paracrine
  • Receptor
  • SV1

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Christodoulou, C., Schally, A. V., Chatzistamou, I., Kondi-Pafiti, A., Lamnissou, K., Kouloheri, S., ... Kiaris, H. (2006). Expression of growth hormone-releasing hormone (GHRH) and splice variant of GHRH receptors in normal mouse tissues. Regulatory Peptides, 136(1-3), 105-108. https://doi.org/10.1016/j.regpep.2006.05.001

Expression of growth hormone-releasing hormone (GHRH) and splice variant of GHRH receptors in normal mouse tissues. / Christodoulou, C.; Schally, Andrew V; Chatzistamou, I.; Kondi-Pafiti, A.; Lamnissou, K.; Kouloheri, S.; Kalofoutis, A.; Kiaris, H.

In: Regulatory Peptides, Vol. 136, No. 1-3, 11.09.2006, p. 105-108.

Research output: Contribution to journalArticle

Christodoulou, C, Schally, AV, Chatzistamou, I, Kondi-Pafiti, A, Lamnissou, K, Kouloheri, S, Kalofoutis, A & Kiaris, H 2006, 'Expression of growth hormone-releasing hormone (GHRH) and splice variant of GHRH receptors in normal mouse tissues', Regulatory Peptides, vol. 136, no. 1-3, pp. 105-108. https://doi.org/10.1016/j.regpep.2006.05.001
Christodoulou, C. ; Schally, Andrew V ; Chatzistamou, I. ; Kondi-Pafiti, A. ; Lamnissou, K. ; Kouloheri, S. ; Kalofoutis, A. ; Kiaris, H. / Expression of growth hormone-releasing hormone (GHRH) and splice variant of GHRH receptors in normal mouse tissues. In: Regulatory Peptides. 2006 ; Vol. 136, No. 1-3. pp. 105-108.
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