Expression of glutamate receptors on cultured cerebral endothelial cells

István A. Krizbai, Mária A. Deli, Anikó Pestenácz, László Siklós, Csilla Andrea Szabó, Ibolya Edit Andras, Ferenc Joó

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Activation of glutamate receptors has been shown to mediate a large number of neuronal processes such as long-term potentiation and ischemic damage. In addition to neurons and glia, glutamate receptors may occur on cerebral endothelial cells (CECs). The aim of the present study was to determine which glutamate receptors are expressed in CECs and to demonstrate the functional presence of such channels. By using reverse transcriptase- polymerase chain reaction, we showed that primary cultures of rat CECs express N-methyl-D-aspartate (NMDA) receptors (NR1 subunit, which is necessary for the formation of functional NMDA receptors, and NR2A-C subunits), 2-amino-3-(3-hydroxy-5-methyl-4-isoxazolyl-propionate (AMPA) receptors (GLUR1-4 subunits), and metabotropic receptors (mGLUR). Exposure of the cultures to 2 mM glutamate, a well-established mediator of ischemic damage, for 30 min increased significantly the phosphorylation of calcium/calmodulin-dependent protein kinase II even after 10- and 60-min recovery times. This effect could be prevented by the NMDA blocker MK-801. The presence of multiple glutamate receptor types may confer a finely tuned responsiveness of the cerebral endothelium to glutamate in physiological and pathological conditions.

Original languageEnglish
Pages (from-to)814-819
Number of pages6
JournalJournal of Neuroscience Research
Volume54
Issue number6
DOIs
StatePublished - Dec 15 1998
Externally publishedYes

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Glutamate Receptors
Endothelial Cells
Calcium-Calmodulin-Dependent Protein Kinase Type 2
N-Methyl-D-Aspartate Receptors
Glutamic Acid
Calcium-Calmodulin-Dependent Protein Kinases
AMPA Receptors
Dizocilpine Maleate
Long-Term Potentiation
Propionates
N-Methylaspartate
Reverse Transcriptase Polymerase Chain Reaction
Neuroglia
Endothelium
Phosphorylation
Neurons

Keywords

  • AMPA receptor
  • Blood-brain barrier
  • Glutamate
  • Metabotropic receptor
  • NMDA receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of glutamate receptors on cultured cerebral endothelial cells. / Krizbai, István A.; Deli, Mária A.; Pestenácz, Anikó; Siklós, László; Szabó, Csilla Andrea; Andras, Ibolya Edit; Joó, Ferenc.

In: Journal of Neuroscience Research, Vol. 54, No. 6, 15.12.1998, p. 814-819.

Research output: Contribution to journalArticle

Krizbai, István A. ; Deli, Mária A. ; Pestenácz, Anikó ; Siklós, László ; Szabó, Csilla Andrea ; Andras, Ibolya Edit ; Joó, Ferenc. / Expression of glutamate receptors on cultured cerebral endothelial cells. In: Journal of Neuroscience Research. 1998 ; Vol. 54, No. 6. pp. 814-819.
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