Expression of glucose transporter-1 in cervical cancer and its precursors

Luis E. Mendez, Natalina Manci, Guilherme Cantuaria, Orlando W Gomez-Marin, Manuel Penalver, Paul G Braunschweiger, Mehrdad Nadji

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Objective. Increased glucose uptake and utilization is a known phenomenon exhibited by malignant cells. Overexpression of the glucose transporter protein family is thought to be the principal mechanism by which these cells achieve up-regulation. Our purpose is to determine glucose transporter-1 (GLUT 1) expression in squamous carcinoma of the cervix and precursor lesions. Methods. Archival histologic sections were obtained from 31 cases of invasive squamous cell carcinoma (SCC) of the uterine cervix, 15 cases of high-grade cervical intraepithelial neoplasia, 5 cases of low-grade, and 9 normal cervices. Immunohistochemistry for GLUT 1 protein was performed using polyclonal GLUT 1 antibody (Dako, Carpinteria, CA) and the labeled streptavidin-biotin procedure. Results. Compared to the internal control, the pattern of staining varied from weak (1+) to strong (3+) reactions. In normal cervix, 1+ GLUT 1 staining was seen in the basal cells of the squamous epithelium. All 31 (100%) cases of SCC were positive for GLUT 1. Positive reactions seemed more intense in tumor cells that were farther away from the stromal blood supply. There was a correlation between intensity of reaction for GLUT 1 and histologic grade of tumor (P = 0.0027) and with progression from normal or dysplastic lesions to invasive cancer (P = 0.0001). Intensity was a predictor of the presence of poorly differentiated tumor type. Low-grade CIN staining was seen in less than one-third of the epithelium, while in high-grade lesions the reaction was present in over one-half of the epithelium. Conclusions. GLUT 1 is overexpressed in cervical carcinoma. The process appears to be related to grade of tumor but not to the progression from preneoplastic lesions. The results suggest that GLUT 1 overexpression is a late phenomenon in cellular transformation. Furthermore, the possible relation of expression to tumor blood supply suggests that the malignant cells may have an adaptive environmental ability to compensate for a compromised microenvironment.

Original languageEnglish
Pages (from-to)138-143
Number of pages6
JournalGynecologic Oncology
Volume86
Issue number2
DOIs
StatePublished - Sep 11 2002

Fingerprint

Facilitative Glucose Transport Proteins
Uterine Cervical Neoplasms
Cervix Uteri
Squamous Cell Carcinoma
Neoplasms
Epithelium
Staining and Labeling
Cervical Intraepithelial Neoplasia
Streptavidin
Biotin
Proteins
Up-Regulation
Epithelial Cells
Immunohistochemistry
Carcinoma
Glucose
Antibodies

Keywords

  • Cervical cancer
  • Cervical intraepithelial neoplasia
  • Glucose transporters
  • GLUT 1
  • Immunohistochemistry

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Oncology

Cite this

Mendez, L. E., Manci, N., Cantuaria, G., Gomez-Marin, O. W., Penalver, M., Braunschweiger, P. G., & Nadji, M. (2002). Expression of glucose transporter-1 in cervical cancer and its precursors. Gynecologic Oncology, 86(2), 138-143. https://doi.org/10.1006/gyno.2002.6745

Expression of glucose transporter-1 in cervical cancer and its precursors. / Mendez, Luis E.; Manci, Natalina; Cantuaria, Guilherme; Gomez-Marin, Orlando W; Penalver, Manuel; Braunschweiger, Paul G; Nadji, Mehrdad.

In: Gynecologic Oncology, Vol. 86, No. 2, 11.09.2002, p. 138-143.

Research output: Contribution to journalArticle

Mendez, LE, Manci, N, Cantuaria, G, Gomez-Marin, OW, Penalver, M, Braunschweiger, PG & Nadji, M 2002, 'Expression of glucose transporter-1 in cervical cancer and its precursors', Gynecologic Oncology, vol. 86, no. 2, pp. 138-143. https://doi.org/10.1006/gyno.2002.6745
Mendez LE, Manci N, Cantuaria G, Gomez-Marin OW, Penalver M, Braunschweiger PG et al. Expression of glucose transporter-1 in cervical cancer and its precursors. Gynecologic Oncology. 2002 Sep 11;86(2):138-143. https://doi.org/10.1006/gyno.2002.6745
Mendez, Luis E. ; Manci, Natalina ; Cantuaria, Guilherme ; Gomez-Marin, Orlando W ; Penalver, Manuel ; Braunschweiger, Paul G ; Nadji, Mehrdad. / Expression of glucose transporter-1 in cervical cancer and its precursors. In: Gynecologic Oncology. 2002 ; Vol. 86, No. 2. pp. 138-143.
@article{ec35ac4855be462fb60f3929752a5eb1,
title = "Expression of glucose transporter-1 in cervical cancer and its precursors",
abstract = "Objective. Increased glucose uptake and utilization is a known phenomenon exhibited by malignant cells. Overexpression of the glucose transporter protein family is thought to be the principal mechanism by which these cells achieve up-regulation. Our purpose is to determine glucose transporter-1 (GLUT 1) expression in squamous carcinoma of the cervix and precursor lesions. Methods. Archival histologic sections were obtained from 31 cases of invasive squamous cell carcinoma (SCC) of the uterine cervix, 15 cases of high-grade cervical intraepithelial neoplasia, 5 cases of low-grade, and 9 normal cervices. Immunohistochemistry for GLUT 1 protein was performed using polyclonal GLUT 1 antibody (Dako, Carpinteria, CA) and the labeled streptavidin-biotin procedure. Results. Compared to the internal control, the pattern of staining varied from weak (1+) to strong (3+) reactions. In normal cervix, 1+ GLUT 1 staining was seen in the basal cells of the squamous epithelium. All 31 (100{\%}) cases of SCC were positive for GLUT 1. Positive reactions seemed more intense in tumor cells that were farther away from the stromal blood supply. There was a correlation between intensity of reaction for GLUT 1 and histologic grade of tumor (P = 0.0027) and with progression from normal or dysplastic lesions to invasive cancer (P = 0.0001). Intensity was a predictor of the presence of poorly differentiated tumor type. Low-grade CIN staining was seen in less than one-third of the epithelium, while in high-grade lesions the reaction was present in over one-half of the epithelium. Conclusions. GLUT 1 is overexpressed in cervical carcinoma. The process appears to be related to grade of tumor but not to the progression from preneoplastic lesions. The results suggest that GLUT 1 overexpression is a late phenomenon in cellular transformation. Furthermore, the possible relation of expression to tumor blood supply suggests that the malignant cells may have an adaptive environmental ability to compensate for a compromised microenvironment.",
keywords = "Cervical cancer, Cervical intraepithelial neoplasia, Glucose transporters, GLUT 1, Immunohistochemistry",
author = "Mendez, {Luis E.} and Natalina Manci and Guilherme Cantuaria and Gomez-Marin, {Orlando W} and Manuel Penalver and Braunschweiger, {Paul G} and Mehrdad Nadji",
year = "2002",
month = "9",
day = "11",
doi = "10.1006/gyno.2002.6745",
language = "English",
volume = "86",
pages = "138--143",
journal = "Gynecologic Oncology",
issn = "0090-8258",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Expression of glucose transporter-1 in cervical cancer and its precursors

AU - Mendez, Luis E.

AU - Manci, Natalina

AU - Cantuaria, Guilherme

AU - Gomez-Marin, Orlando W

AU - Penalver, Manuel

AU - Braunschweiger, Paul G

AU - Nadji, Mehrdad

PY - 2002/9/11

Y1 - 2002/9/11

N2 - Objective. Increased glucose uptake and utilization is a known phenomenon exhibited by malignant cells. Overexpression of the glucose transporter protein family is thought to be the principal mechanism by which these cells achieve up-regulation. Our purpose is to determine glucose transporter-1 (GLUT 1) expression in squamous carcinoma of the cervix and precursor lesions. Methods. Archival histologic sections were obtained from 31 cases of invasive squamous cell carcinoma (SCC) of the uterine cervix, 15 cases of high-grade cervical intraepithelial neoplasia, 5 cases of low-grade, and 9 normal cervices. Immunohistochemistry for GLUT 1 protein was performed using polyclonal GLUT 1 antibody (Dako, Carpinteria, CA) and the labeled streptavidin-biotin procedure. Results. Compared to the internal control, the pattern of staining varied from weak (1+) to strong (3+) reactions. In normal cervix, 1+ GLUT 1 staining was seen in the basal cells of the squamous epithelium. All 31 (100%) cases of SCC were positive for GLUT 1. Positive reactions seemed more intense in tumor cells that were farther away from the stromal blood supply. There was a correlation between intensity of reaction for GLUT 1 and histologic grade of tumor (P = 0.0027) and with progression from normal or dysplastic lesions to invasive cancer (P = 0.0001). Intensity was a predictor of the presence of poorly differentiated tumor type. Low-grade CIN staining was seen in less than one-third of the epithelium, while in high-grade lesions the reaction was present in over one-half of the epithelium. Conclusions. GLUT 1 is overexpressed in cervical carcinoma. The process appears to be related to grade of tumor but not to the progression from preneoplastic lesions. The results suggest that GLUT 1 overexpression is a late phenomenon in cellular transformation. Furthermore, the possible relation of expression to tumor blood supply suggests that the malignant cells may have an adaptive environmental ability to compensate for a compromised microenvironment.

AB - Objective. Increased glucose uptake and utilization is a known phenomenon exhibited by malignant cells. Overexpression of the glucose transporter protein family is thought to be the principal mechanism by which these cells achieve up-regulation. Our purpose is to determine glucose transporter-1 (GLUT 1) expression in squamous carcinoma of the cervix and precursor lesions. Methods. Archival histologic sections were obtained from 31 cases of invasive squamous cell carcinoma (SCC) of the uterine cervix, 15 cases of high-grade cervical intraepithelial neoplasia, 5 cases of low-grade, and 9 normal cervices. Immunohistochemistry for GLUT 1 protein was performed using polyclonal GLUT 1 antibody (Dako, Carpinteria, CA) and the labeled streptavidin-biotin procedure. Results. Compared to the internal control, the pattern of staining varied from weak (1+) to strong (3+) reactions. In normal cervix, 1+ GLUT 1 staining was seen in the basal cells of the squamous epithelium. All 31 (100%) cases of SCC were positive for GLUT 1. Positive reactions seemed more intense in tumor cells that were farther away from the stromal blood supply. There was a correlation between intensity of reaction for GLUT 1 and histologic grade of tumor (P = 0.0027) and with progression from normal or dysplastic lesions to invasive cancer (P = 0.0001). Intensity was a predictor of the presence of poorly differentiated tumor type. Low-grade CIN staining was seen in less than one-third of the epithelium, while in high-grade lesions the reaction was present in over one-half of the epithelium. Conclusions. GLUT 1 is overexpressed in cervical carcinoma. The process appears to be related to grade of tumor but not to the progression from preneoplastic lesions. The results suggest that GLUT 1 overexpression is a late phenomenon in cellular transformation. Furthermore, the possible relation of expression to tumor blood supply suggests that the malignant cells may have an adaptive environmental ability to compensate for a compromised microenvironment.

KW - Cervical cancer

KW - Cervical intraepithelial neoplasia

KW - Glucose transporters

KW - GLUT 1

KW - Immunohistochemistry

UR - http://www.scopus.com/inward/record.url?scp=0036354858&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036354858&partnerID=8YFLogxK

U2 - 10.1006/gyno.2002.6745

DO - 10.1006/gyno.2002.6745

M3 - Article

VL - 86

SP - 138

EP - 143

JO - Gynecologic Oncology

JF - Gynecologic Oncology

SN - 0090-8258

IS - 2

ER -