Expression of complement regulatory proteins - CD 35, CD 46, CD 55, and CD 59 - In benign and malignant endometrial tissue

Karuna P. Murray, Shephali Mathure, Rashmi Kaul, Shah Khan, Linda F. Carson, Leo B. Twiggs, Mark G. Martens, Anil Kaul

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Objective. Complement system plays an important role in host defense mechanisms against microorganisms and tumor cells. To protect themselves from autologous complement-mediated damage, normal host tissues express cell membrane-associated complement regulatory proteins (CRPs). To investigate whether neoplastic endometrial tissues overexpress these proteins to escape complement damage, we examined the distribution of complement receptor type 1 (CR1, CD35), membrane cofactor protein (MCP, CD46), decay-accelerating factor (DAF, CD55), and protectin (MACIF, CD59) on frozen endometrial tissue samples. Methods. A total of 54 endometrial tissue samples were collected. Cryosections were obtained of 31 benign and 23 malignant tissue specimens. Tissue sections were stained by immunohistochemical staining procedure using specific antibodies and employing the avidin-biotin technique. Quantitation of the protein content of these CRPs was determined using the Samba 4000 image analysis system. Results. For all four of the CRPs studied, a statistically significant difference in protein expression between the benign and malignant endometrial tissue specimens (P < 0.0001) was observed. Conclusions. Overexpression of all the CRPs studied (CD35, CD46, CD55, CD59) was observed in the malignant as compared with the benign endometrial tissues. The upregulation of these CRPs may promote resistance of the endometrial malignant tissue to complement-mediated damage, thereby allowing the tumor cells to escape from cytolysis and thus promoting carcinogenesis.

Original languageEnglish
Pages (from-to)176-182
Number of pages7
JournalGynecologic Oncology
Volume76
Issue number2
DOIs
StatePublished - Feb 1 2000
Externally publishedYes

Fingerprint

Complement System Proteins
CD59 Antigens
CD46 Antigens
CD55 Antigens
Complement Receptors
Proteins
Avidin
Biotin
Neoplasms
Membrane Proteins
Carcinogenesis
Up-Regulation
Cell Membrane
Staining and Labeling
Antibodies

Keywords

  • Complement receptor type 1 (CR1, CD35)
  • Decay-accelerating factor (DAF, CD55)
  • Endometrial cancer
  • Membrane cofactor protein (MCP, CD46)
  • Protectin (CD59)

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Oncology

Cite this

Expression of complement regulatory proteins - CD 35, CD 46, CD 55, and CD 59 - In benign and malignant endometrial tissue. / Murray, Karuna P.; Mathure, Shephali; Kaul, Rashmi; Khan, Shah; Carson, Linda F.; Twiggs, Leo B.; Martens, Mark G.; Kaul, Anil.

In: Gynecologic Oncology, Vol. 76, No. 2, 01.02.2000, p. 176-182.

Research output: Contribution to journalArticle

Murray, KP, Mathure, S, Kaul, R, Khan, S, Carson, LF, Twiggs, LB, Martens, MG & Kaul, A 2000, 'Expression of complement regulatory proteins - CD 35, CD 46, CD 55, and CD 59 - In benign and malignant endometrial tissue', Gynecologic Oncology, vol. 76, no. 2, pp. 176-182. https://doi.org/10.1006/gyno.1999.5614
Murray, Karuna P. ; Mathure, Shephali ; Kaul, Rashmi ; Khan, Shah ; Carson, Linda F. ; Twiggs, Leo B. ; Martens, Mark G. ; Kaul, Anil. / Expression of complement regulatory proteins - CD 35, CD 46, CD 55, and CD 59 - In benign and malignant endometrial tissue. In: Gynecologic Oncology. 2000 ; Vol. 76, No. 2. pp. 176-182.
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AU - Mathure, Shephali

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AU - Khan, Shah

AU - Carson, Linda F.

AU - Twiggs, Leo B.

AU - Martens, Mark G.

AU - Kaul, Anil

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