Expression of chimeric tRNA-driven antisense transcripts renders NIH 3T3 cells highly resistant to moloney murine leukemia virus replication

Bruce A. Sullenger, Thomas C. Lee, Clayton A. Smith, Grace E. Ungers, Eli Gilboa

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

NIH 3T3 cells infected with Moloney murine leukemia virus (MoMLV) express high levels of virus-specific RNA. To inhibit replication of the virus, we stably introduced chimeric tRNA genes encoding antisense templates into NIH 3T3 cells via a retroviral vector. Efficient expression of hybrid tRNA-MoMLV antisense transcripts and inhibition of MoMLV replication were dependent on the use of a particular type of retroviral vector, the double-copy vector, in which the chimeric tRNA gene was inserted in the 3′ long terminal repeat. MoMLV replication was inhibited up to 97% in cells expressing antisense RNA corresponding to the gag gene and less than twofold in cells expressing antisense RNA corresponding to the pol gene. RNA and protein analyses suggest that inhibition was exerted at the level of translation. These results suggest that RNA polymerase III-based antisense inhibition systems can be used to inhibit highly expressed viral genes and render cells resistant to viral replication via intracellular immunization strategies.

Original languageEnglish
Pages (from-to)6512-6523
Number of pages12
JournalMolecular and Cellular Biology
Volume10
Issue number12
StatePublished - Dec 1 1990
Externally publishedYes

Fingerprint

Moloney murine leukemia virus
NIH 3T3 Cells
Virus Replication
Transfer RNA
Antisense RNA
pol Genes
gag Genes
RNA Polymerase III
Viral Genes
Terminal Repeat Sequences
RNA Viruses
Genes
Immunization
RNA
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Expression of chimeric tRNA-driven antisense transcripts renders NIH 3T3 cells highly resistant to moloney murine leukemia virus replication. / Sullenger, Bruce A.; Lee, Thomas C.; Smith, Clayton A.; Ungers, Grace E.; Gilboa, Eli.

In: Molecular and Cellular Biology, Vol. 10, No. 12, 01.12.1990, p. 6512-6523.

Research output: Contribution to journalArticle

Sullenger, Bruce A. ; Lee, Thomas C. ; Smith, Clayton A. ; Ungers, Grace E. ; Gilboa, Eli. / Expression of chimeric tRNA-driven antisense transcripts renders NIH 3T3 cells highly resistant to moloney murine leukemia virus replication. In: Molecular and Cellular Biology. 1990 ; Vol. 10, No. 12. pp. 6512-6523.
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