Expression of brain-derived neurotrophic factor is regulated by the Wnt signaling pathway

Hyun Yi, Jianfei Hu, Jiang Qian, Abigail S Hackam

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Brain-derived neurotrophic factor (BDNF) is a well characterized neurotrophin that mediates a wide variety of activities in the central nervous system, including neuronal differentiation, neuroprotection, and synaptic plasticity. The canonical Wnt signaling pathway is a critical regulator of embryonic development and homeostasis in adult tissues. Our group and others recently demonstrated that Wnt signaling induces BDNF expression in neurons and glia. However, the precise relationship between BDNF and Wnt signaling pathways is not understood. Here, we investigated Wnt signaling regulation of BDNF at the transcriptional level using a combination of bioinformatics and molecular analyses. Analysis of the BDNF gene promoter identified seven binding motifs for Wnt-dependent TCF/LEF transcription factors. Furthermore, specific BDNF promoters were induced by the Wnt3a ligand using chloramphenicol acetyl transferase reporter assays and a dominant-negative TCF4 gene reduced Wnt3a-mediated induction. Finally, Wnt3a induced expression of BDNF and other members of the BDNF signaling pathway in glia cells. Therefore, these data indicate that BDNF is a direct target of Wnt signaling, which provides a new insight into the interaction between two essential signaling pathways.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalNeuroReport
Volume23
Issue number3
DOIs
StatePublished - Feb 15 2012

Fingerprint

Wnt Signaling Pathway
Brain-Derived Neurotrophic Factor
TCF Transcription Factors
Neuroglia
Neuronal Plasticity
Nerve Growth Factors
Chloramphenicol
Transferases
Computational Biology
Genes
Embryonic Development
Homeostasis
Central Nervous System
Ligands
Neurons

Keywords

  • Brain-derived neurotrophic factor
  • Glia
  • Retina
  • TCF/LEF
  • Wnt signaling

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of brain-derived neurotrophic factor is regulated by the Wnt signaling pathway. / Yi, Hyun; Hu, Jianfei; Qian, Jiang; Hackam, Abigail S.

In: NeuroReport, Vol. 23, No. 3, 15.02.2012, p. 189-194.

Research output: Contribution to journalArticle

Yi, Hyun ; Hu, Jianfei ; Qian, Jiang ; Hackam, Abigail S. / Expression of brain-derived neurotrophic factor is regulated by the Wnt signaling pathway. In: NeuroReport. 2012 ; Vol. 23, No. 3. pp. 189-194.
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