Expression of aquaporin-1 (AQP-1) in rat heart

J. S. Zheng, Y. Dai, J. Li, X. Long, L. O'Neill, Z. Li, W. J. Lederer, H. Cheng, B. J. Baum, E. G. Lakatta, M. T. Crow, M. O. Boluyt

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: AQP-1, a channel-forming integral membrane protein of 28 kDa prevalent in red blood cells and renal proximal tubules, was recently shown to be expressed in rat heart. Aims: Our purpose was to characterise the expression of the AQP-1 gene in rat heart with respect to cell type, developmental stage and pathophysiological condition. Methods: To determine in which heart cell type the water channel was expressed, we measured AQP-1 mRNA and protein levels and immunolocalised protein in freshly isolated myocytes from adult rats and cultured myocytes and fibroblasts from neonatal rats. Results: Northern blot analysis showed that AQP-1 mRNA is expressed in adult cardiac myocytes, neonatal myocytes, and neonatal cardiac fibroblasts of rats at levels nearly as high as those observed in rat kidney. Western blot analysis, however, detected AQP-1 protein only in purified adult and neonatal cardiac myocytes at levels markedly less than kidney. Immunohistochemistry and confocal imaging localised AQP-1 protein to the sarcolemmal membrane of myocytes. Since the expression of other membrane-spanning proteins is altered during hypertrophy, we determined the level of AQP-1 mRNA in hearts of aortic-constricted (AC) rats. The level of AQP-1 mRNA decreased progressively after AC, and was 42% less than the level in left ventricles (LVs) of sham-operated control rats after 3 days of AC. Conclusions: These data indicate that AQP-1 is expressed in cardiac myocytes of adult and neonatal rats, and its expression is modulated in the rat heart during pressure-overload hypertrophy.

Original languageEnglish (US)
Pages (from-to)36-43
Number of pages8
JournalAsia Pacific Heart Journal
Volume8
Issue number1
DOIs
StatePublished - 1999
Externally publishedYes

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Aquaporin 1
Cardiac Myocytes
Muscle Cells
Messenger RNA
Hypertrophy
Fibroblasts
Kidney
Proximal Kidney Tubule
Aquaporins
Northern Blotting
Heart Ventricles
Membrane Proteins
Erythrocytes
Western Blotting
Immunohistochemistry

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Zheng, J. S., Dai, Y., Li, J., Long, X., O'Neill, L., Li, Z., ... Boluyt, M. O. (1999). Expression of aquaporin-1 (AQP-1) in rat heart. Asia Pacific Heart Journal, 8(1), 36-43. https://doi.org/10.1016/S1328-0163(99)90007-6

Expression of aquaporin-1 (AQP-1) in rat heart. / Zheng, J. S.; Dai, Y.; Li, J.; Long, X.; O'Neill, L.; Li, Z.; Lederer, W. J.; Cheng, H.; Baum, B. J.; Lakatta, E. G.; Crow, M. T.; Boluyt, M. O.

In: Asia Pacific Heart Journal, Vol. 8, No. 1, 1999, p. 36-43.

Research output: Contribution to journalArticle

Zheng, JS, Dai, Y, Li, J, Long, X, O'Neill, L, Li, Z, Lederer, WJ, Cheng, H, Baum, BJ, Lakatta, EG, Crow, MT & Boluyt, MO 1999, 'Expression of aquaporin-1 (AQP-1) in rat heart', Asia Pacific Heart Journal, vol. 8, no. 1, pp. 36-43. https://doi.org/10.1016/S1328-0163(99)90007-6
Zheng, J. S. ; Dai, Y. ; Li, J. ; Long, X. ; O'Neill, L. ; Li, Z. ; Lederer, W. J. ; Cheng, H. ; Baum, B. J. ; Lakatta, E. G. ; Crow, M. T. ; Boluyt, M. O. / Expression of aquaporin-1 (AQP-1) in rat heart. In: Asia Pacific Heart Journal. 1999 ; Vol. 8, No. 1. pp. 36-43.
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abstract = "Background: AQP-1, a channel-forming integral membrane protein of 28 kDa prevalent in red blood cells and renal proximal tubules, was recently shown to be expressed in rat heart. Aims: Our purpose was to characterise the expression of the AQP-1 gene in rat heart with respect to cell type, developmental stage and pathophysiological condition. Methods: To determine in which heart cell type the water channel was expressed, we measured AQP-1 mRNA and protein levels and immunolocalised protein in freshly isolated myocytes from adult rats and cultured myocytes and fibroblasts from neonatal rats. Results: Northern blot analysis showed that AQP-1 mRNA is expressed in adult cardiac myocytes, neonatal myocytes, and neonatal cardiac fibroblasts of rats at levels nearly as high as those observed in rat kidney. Western blot analysis, however, detected AQP-1 protein only in purified adult and neonatal cardiac myocytes at levels markedly less than kidney. Immunohistochemistry and confocal imaging localised AQP-1 protein to the sarcolemmal membrane of myocytes. Since the expression of other membrane-spanning proteins is altered during hypertrophy, we determined the level of AQP-1 mRNA in hearts of aortic-constricted (AC) rats. The level of AQP-1 mRNA decreased progressively after AC, and was 42{\%} less than the level in left ventricles (LVs) of sham-operated control rats after 3 days of AC. Conclusions: These data indicate that AQP-1 is expressed in cardiac myocytes of adult and neonatal rats, and its expression is modulated in the rat heart during pressure-overload hypertrophy.",
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AU - Zheng, J. S.

AU - Dai, Y.

AU - Li, J.

AU - Long, X.

AU - O'Neill, L.

AU - Li, Z.

AU - Lederer, W. J.

AU - Cheng, H.

AU - Baum, B. J.

AU - Lakatta, E. G.

AU - Crow, M. T.

AU - Boluyt, M. O.

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