Expression of antigens of the carcinoembryonic antigen family on B cell lymphomas and epstein-barr virus immortalized B cell lines

Wasif Noor Khan, Sten Hammarström, Teresa Ramos

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Expression of antigens of the carcinoembryonic (CEA) family on B cell lymphomas and Epstein-Barr virus (EBV) immortalized B cell lines was studied using two CEA reactive mAbs. Results from experiments where five B cell lymphomas and two immortalized B cell lines, one from fetal liver and one from normal blood, were analyzed by flow cytometry, showed that all cell lines were strongly reactive with both mAbs. Glycoproteins of 66-90kDa were identified in cell lysates and spent medium of metabolically labelled cells, immunoprecipitated with the two anti-CEA mAbs and analyzed by SDS-PAGE autoradiography. In cell lysates, a polyclonal anti-CEA antibody reacted with two molecules of 78 and 90kDa. EBV negative lymphomas, converted, in vitro, with EBV, expressed significantly higher glycoprotein levels, as compared with the parent cell lines. Furthermore, two additional glycoproteins of 45 and 33kDa were found in lysates from these cells. Northern analysis of mRNA from four of the lymphoma and one of the EBV immortalized B cell lines, using a probe which hybridizes with all CEA family members, showed two CEA related transcripts of 3.9 and 2.2kb. Based on the sizes of the transcripts and of the glycoproteins, it was concluded that some of the CEA antigens expressed on B cells were, most likely, alternatively spliced forms of biliary glycoprotein.

Original languageEnglish (US)
Pages (from-to)265-270
Number of pages6
JournalInternational Immunology
Volume5
Issue number3
DOIs
StatePublished - Mar 1 1993

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Keywords

  • Biliary glycoprotein
  • Carcinoembryonic antigen
  • Epstein-Barr virus

ASJC Scopus subject areas

  • Immunology
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Transplantation

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