Expression of alternate forms of brain opioid 'orphan' receptor mRNA in activated human peripheral blood lymphocytes and lymphocytic cell lines

M. J. Wick, S. R. Minnerath, S. Roy, S. Ramakrishnan, H. H. Loh

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

We screened a PHA (phytohemagglutinin)-activated human lymphocyte cDNA library for clones with homology to the recently cloned brain opioid receptors. A cDNA clone, AT7-5EU, was isolated which encodes the opioid 'orphan' receptor, a molecule with very high homology to the opioid receptor gene family, but which has not been shown to bind opioids or any other known compounds. The protein coding region of AT7-5EU has complete homology with a reported opioid 'orphan' clone isolated from human brain, but the 5′ untranslated regions of AT7-5EU and the human brain clones are divergent, suggesting mechanisms for tissue-specific expression of this receptor. Northern analysis of AT7-5EU mRNA demonstrates the expression of this message in human lymphocytic cell lines of both B-and T-cell lineages. Furthermore, analysis of mRNA from human peripheral blood lymphocytes demonstrates that activation of the lymphocytes with PHA results in at least a 10-fold induction of the AT7-5EU message. These results suggest that the opioid 'orphan' receptor may have an important immunological function in addition to its function in the nervous system.

Original languageEnglish (US)
Pages (from-to)342-347
Number of pages6
JournalMolecular Brain Research
Volume32
Issue number2
DOIs
StatePublished - Sep 1995
Externally publishedYes

Keywords

  • Gene activation
  • Lymphocyte activation
  • Northern analysis
  • Tissue-specific expression
  • cDNA cloning

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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