Expression of a functional secreted YopN-TyeA hybrid protein in Yersinia pestis is the result of a +1 translational frameshift event

Franco Ferracci, James B. Day, Heather J. Ezelle, Gregory V Plano

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

YopN is a secreted protein that prior to secretion directly interacts with the cytosolic SycN/YscB chaperone complex and TyeA. This study identifies a secreted YopN-TyeA hybrid protein that is expressed by Yersinia pestis, but not by Yersinia enterocolitica. DNA sequence analysis and site-directed mutagenesis studies demonstrate that the hybrid protein is the result of a +1 translational frameshift event.

Original languageEnglish
Pages (from-to)5160-5166
Number of pages7
JournalJournal of Bacteriology
Volume186
Issue number15
DOIs
StatePublished - Aug 1 2004

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Yersinia pestis
Yersinia enterocolitica
Proteins
Site-Directed Mutagenesis
DNA Sequence Analysis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Expression of a functional secreted YopN-TyeA hybrid protein in Yersinia pestis is the result of a +1 translational frameshift event. / Ferracci, Franco; Day, James B.; Ezelle, Heather J.; Plano, Gregory V.

In: Journal of Bacteriology, Vol. 186, No. 15, 01.08.2004, p. 5160-5166.

Research output: Contribution to journalArticle

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