Expression of 92 kD type IV collagenase/gelatinase B in human osteoclasts

Anne L. Wucherpfennig, Yi Ping Li, William G. Stetler-Stevenson, Andrew Rosenberg, Philip Stashenko

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

The digestion of type I collagen is an essential step in bone resorption. It is well established that osteoclasts solubilize the mineral phase of bone during the resorptive process, but the mechanism by which they degrade type I collagen, the major proteinaceous component of bone, is controversial. Differential screening of a human osteoclastoma cDNA library was performed to characterize genes specifically expressed in osteoclasts. A large number of cDNA clones obtained by this procedure were found to represent 92 kD type IV collagenase (gelatinase B; MMP-9, EC 3.4.24.35), as well as tartrate- resistant acid phosphatase. In situ hybridization localized mRNA for gelatinase B to multinucleated giant cells in human osteoclastomas. Gelatinase B immunoreactivity was demonstrated in giant cells from eight of eight osteoclastomas, osteoclasts in normal bone, and osteoclasts of Paget's disease by use of a polyclonal antiserum raised against a synthetic gelatinase B peptide. In contrast, no immunoreactivity for 72 kD type IV collagenase (gelatinase A; MMP-2, EC 3.4.24.24), which is the product of a separate gene, was detected in osteoclastomas or normal osteoclasts. We propose that the 92 kD type IV collagenase/gelatinase B plays an important role in the resorption of collagen during bone remodeling.

Original languageEnglish
Pages (from-to)549-556
Number of pages8
JournalJournal of Bone and Mineral Research
Volume9
Issue number4
StatePublished - Apr 1 1994
Externally publishedYes

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Matrix Metalloproteinase 9
Collagenases
Osteoclasts
Giant Cells
Collagen Type I
Matrix Metalloproteinases
Osteitis Deformans
Bone and Bones
Bone Remodeling
Matrix Metalloproteinase 2
Bone Resorption
Gene Library
Genes
In Situ Hybridization
Minerals
Immune Sera
Digestion
Collagen
Complementary DNA
Clone Cells

ASJC Scopus subject areas

  • Surgery

Cite this

Wucherpfennig, A. L., Li, Y. P., Stetler-Stevenson, W. G., Rosenberg, A., & Stashenko, P. (1994). Expression of 92 kD type IV collagenase/gelatinase B in human osteoclasts. Journal of Bone and Mineral Research, 9(4), 549-556.

Expression of 92 kD type IV collagenase/gelatinase B in human osteoclasts. / Wucherpfennig, Anne L.; Li, Yi Ping; Stetler-Stevenson, William G.; Rosenberg, Andrew; Stashenko, Philip.

In: Journal of Bone and Mineral Research, Vol. 9, No. 4, 01.04.1994, p. 549-556.

Research output: Contribution to journalArticle

Wucherpfennig, AL, Li, YP, Stetler-Stevenson, WG, Rosenberg, A & Stashenko, P 1994, 'Expression of 92 kD type IV collagenase/gelatinase B in human osteoclasts', Journal of Bone and Mineral Research, vol. 9, no. 4, pp. 549-556.
Wucherpfennig AL, Li YP, Stetler-Stevenson WG, Rosenberg A, Stashenko P. Expression of 92 kD type IV collagenase/gelatinase B in human osteoclasts. Journal of Bone and Mineral Research. 1994 Apr 1;9(4):549-556.
Wucherpfennig, Anne L. ; Li, Yi Ping ; Stetler-Stevenson, William G. ; Rosenberg, Andrew ; Stashenko, Philip. / Expression of 92 kD type IV collagenase/gelatinase B in human osteoclasts. In: Journal of Bone and Mineral Research. 1994 ; Vol. 9, No. 4. pp. 549-556.
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