Expression of 67 kDa laminin receptor in human breast cancer cells: regulation by progestins

Yuenian E. Shi, Jeff Torri, Lynn Yieh, Mark E. Sobel, Yoshihiko Yamada, Marc E Lippman, Robert B. Dickson, Erik W. Thompson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The level of 67 kDa laminin receptor (67LR) expression on breast and colon tumor cell surfaces was previously shown to be correlated with the capacity of tumor cells to metastasize. In the present work we investigate the effects of progestins and estrogen on the expression of 67LR in two sublines of the T47D human breast cancer cells: weakly tumorigenic, poorly invasive parental T47D cells and a highly tumorigenic, more invasive T47Dco subclone. Inmmunoblotting with an affinity purified antibody directed against a synthetic peptide recognizes the 67LR in these cells. 67LR expression in the T47Dco subclone is 5,5-fold higher than in their parental T47D cells. Treatment of T47D cells with 1 nM of the synthetic progestin R5020 results in a 4-fold increase in 67LR protein expression. Estrogen also induced 67LR expression, but only by 1.5-fold. The progestin-stimulated expression of the 67LR correlates with a 4.3-fold increase in attachment of T47D cells to laminin. A monoclonal antibody, mAb 13, directed against β1 integrin, completely blocks the attachment of T47D cells to fibronectin, only partially inhibits the attachment of T47D cells to laminin, and appears not to affect the progestin-stimulated laminin attachment of T47D cells. A new antiprogestin, ZK 112.993, significantly inhibits both progestin-stimulated 67LR expression and the increased attachment to laminin. These results suggest a possible role for progestin in mediating one of the multiple events thought to be important in metastasis of steroid receptor positive human breast cancer cells.

Original languageEnglish
Pages (from-to)251-261
Number of pages11
JournalClinical & Experimental Metastasis
Volume11
Issue number3
DOIs
StatePublished - May 1 1993
Externally publishedYes

Fingerprint

Laminin Receptors
Progestins
Breast Neoplasms
Laminin
Estrogens
Promegestone
Progesterone Congeners
Antibody Affinity
Steroid Receptors
Fibronectins
Integrins

Keywords

  • adhesion
  • antiprogestins
  • human breast cancer
  • laminin receptor
  • progestins

ASJC Scopus subject areas

  • Cancer Research

Cite this

Shi, Y. E., Torri, J., Yieh, L., Sobel, M. E., Yamada, Y., Lippman, M. E., ... Thompson, E. W. (1993). Expression of 67 kDa laminin receptor in human breast cancer cells: regulation by progestins. Clinical & Experimental Metastasis, 11(3), 251-261. https://doi.org/10.1007/BF00121168

Expression of 67 kDa laminin receptor in human breast cancer cells : regulation by progestins. / Shi, Yuenian E.; Torri, Jeff; Yieh, Lynn; Sobel, Mark E.; Yamada, Yoshihiko; Lippman, Marc E; Dickson, Robert B.; Thompson, Erik W.

In: Clinical & Experimental Metastasis, Vol. 11, No. 3, 01.05.1993, p. 251-261.

Research output: Contribution to journalArticle

Shi, YE, Torri, J, Yieh, L, Sobel, ME, Yamada, Y, Lippman, ME, Dickson, RB & Thompson, EW 1993, 'Expression of 67 kDa laminin receptor in human breast cancer cells: regulation by progestins', Clinical & Experimental Metastasis, vol. 11, no. 3, pp. 251-261. https://doi.org/10.1007/BF00121168
Shi, Yuenian E. ; Torri, Jeff ; Yieh, Lynn ; Sobel, Mark E. ; Yamada, Yoshihiko ; Lippman, Marc E ; Dickson, Robert B. ; Thompson, Erik W. / Expression of 67 kDa laminin receptor in human breast cancer cells : regulation by progestins. In: Clinical & Experimental Metastasis. 1993 ; Vol. 11, No. 3. pp. 251-261.
@article{1a6004cbf26c40bfbe095c6bb4cb0014,
title = "Expression of 67 kDa laminin receptor in human breast cancer cells: regulation by progestins",
abstract = "The level of 67 kDa laminin receptor (67LR) expression on breast and colon tumor cell surfaces was previously shown to be correlated with the capacity of tumor cells to metastasize. In the present work we investigate the effects of progestins and estrogen on the expression of 67LR in two sublines of the T47D human breast cancer cells: weakly tumorigenic, poorly invasive parental T47D cells and a highly tumorigenic, more invasive T47Dco subclone. Inmmunoblotting with an affinity purified antibody directed against a synthetic peptide recognizes the 67LR in these cells. 67LR expression in the T47Dco subclone is 5,5-fold higher than in their parental T47D cells. Treatment of T47D cells with 1 nM of the synthetic progestin R5020 results in a 4-fold increase in 67LR protein expression. Estrogen also induced 67LR expression, but only by 1.5-fold. The progestin-stimulated expression of the 67LR correlates with a 4.3-fold increase in attachment of T47D cells to laminin. A monoclonal antibody, mAb 13, directed against β1 integrin, completely blocks the attachment of T47D cells to fibronectin, only partially inhibits the attachment of T47D cells to laminin, and appears not to affect the progestin-stimulated laminin attachment of T47D cells. A new antiprogestin, ZK 112.993, significantly inhibits both progestin-stimulated 67LR expression and the increased attachment to laminin. These results suggest a possible role for progestin in mediating one of the multiple events thought to be important in metastasis of steroid receptor positive human breast cancer cells.",
keywords = "adhesion, antiprogestins, human breast cancer, laminin receptor, progestins",
author = "Shi, {Yuenian E.} and Jeff Torri and Lynn Yieh and Sobel, {Mark E.} and Yoshihiko Yamada and Lippman, {Marc E} and Dickson, {Robert B.} and Thompson, {Erik W.}",
year = "1993",
month = "5",
day = "1",
doi = "10.1007/BF00121168",
language = "English",
volume = "11",
pages = "251--261",
journal = "Clinical and Experimental Metastasis",
issn = "0262-0898",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Expression of 67 kDa laminin receptor in human breast cancer cells

T2 - regulation by progestins

AU - Shi, Yuenian E.

AU - Torri, Jeff

AU - Yieh, Lynn

AU - Sobel, Mark E.

AU - Yamada, Yoshihiko

AU - Lippman, Marc E

AU - Dickson, Robert B.

AU - Thompson, Erik W.

PY - 1993/5/1

Y1 - 1993/5/1

N2 - The level of 67 kDa laminin receptor (67LR) expression on breast and colon tumor cell surfaces was previously shown to be correlated with the capacity of tumor cells to metastasize. In the present work we investigate the effects of progestins and estrogen on the expression of 67LR in two sublines of the T47D human breast cancer cells: weakly tumorigenic, poorly invasive parental T47D cells and a highly tumorigenic, more invasive T47Dco subclone. Inmmunoblotting with an affinity purified antibody directed against a synthetic peptide recognizes the 67LR in these cells. 67LR expression in the T47Dco subclone is 5,5-fold higher than in their parental T47D cells. Treatment of T47D cells with 1 nM of the synthetic progestin R5020 results in a 4-fold increase in 67LR protein expression. Estrogen also induced 67LR expression, but only by 1.5-fold. The progestin-stimulated expression of the 67LR correlates with a 4.3-fold increase in attachment of T47D cells to laminin. A monoclonal antibody, mAb 13, directed against β1 integrin, completely blocks the attachment of T47D cells to fibronectin, only partially inhibits the attachment of T47D cells to laminin, and appears not to affect the progestin-stimulated laminin attachment of T47D cells. A new antiprogestin, ZK 112.993, significantly inhibits both progestin-stimulated 67LR expression and the increased attachment to laminin. These results suggest a possible role for progestin in mediating one of the multiple events thought to be important in metastasis of steroid receptor positive human breast cancer cells.

AB - The level of 67 kDa laminin receptor (67LR) expression on breast and colon tumor cell surfaces was previously shown to be correlated with the capacity of tumor cells to metastasize. In the present work we investigate the effects of progestins and estrogen on the expression of 67LR in two sublines of the T47D human breast cancer cells: weakly tumorigenic, poorly invasive parental T47D cells and a highly tumorigenic, more invasive T47Dco subclone. Inmmunoblotting with an affinity purified antibody directed against a synthetic peptide recognizes the 67LR in these cells. 67LR expression in the T47Dco subclone is 5,5-fold higher than in their parental T47D cells. Treatment of T47D cells with 1 nM of the synthetic progestin R5020 results in a 4-fold increase in 67LR protein expression. Estrogen also induced 67LR expression, but only by 1.5-fold. The progestin-stimulated expression of the 67LR correlates with a 4.3-fold increase in attachment of T47D cells to laminin. A monoclonal antibody, mAb 13, directed against β1 integrin, completely blocks the attachment of T47D cells to fibronectin, only partially inhibits the attachment of T47D cells to laminin, and appears not to affect the progestin-stimulated laminin attachment of T47D cells. A new antiprogestin, ZK 112.993, significantly inhibits both progestin-stimulated 67LR expression and the increased attachment to laminin. These results suggest a possible role for progestin in mediating one of the multiple events thought to be important in metastasis of steroid receptor positive human breast cancer cells.

KW - adhesion

KW - antiprogestins

KW - human breast cancer

KW - laminin receptor

KW - progestins

UR - http://www.scopus.com/inward/record.url?scp=0027300132&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027300132&partnerID=8YFLogxK

U2 - 10.1007/BF00121168

DO - 10.1007/BF00121168

M3 - Article

C2 - 8472397

AN - SCOPUS:0027300132

VL - 11

SP - 251

EP - 261

JO - Clinical and Experimental Metastasis

JF - Clinical and Experimental Metastasis

SN - 0262-0898

IS - 3

ER -