Expression and purification of Src I from sea anemone Sagartia rosea as a recombinant non-fusion protein

Xiaoyu Jiang, Lisheng Peng, Wenli Yang, Xiaojiang Tang, Wei Liu, Anlong Xu

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The cDNA coding for an acidic actinoporin, Sagartia rosea cytolysin I (Src I), has been isolated, cloned into pGEM-T Easy Vector, and sequenced. The region encoding matured Src I was also cloned into prokaryotic expression vector pBV220 and expressed in Escherichia coli as a non-fusion protein in the form of inclusion body. Through washing and denaturation-renaturation step, the recombinant Src I was purified by Q Sepharose Fast Flow ion exchange chromatography and Phenyl Sepharose hydrophobic interaction chromatography. The two-step purification of Src I was effective, simple, and suitable for isolating large amount of high purity (above 95%) recombinant Src I.

Original languageEnglish (US)
Pages (from-to)161-166
Number of pages6
JournalProtein Expression and Purification
Volume32
Issue number1
DOIs
StatePublished - Nov 2003
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology

Fingerprint Dive into the research topics of 'Expression and purification of Src I from sea anemone Sagartia rosea as a recombinant non-fusion protein'. Together they form a unique fingerprint.

Cite this