Abstract
The prostate gland contains neuroendocrine cells and amidated neuroendocrine peptides whose presence has been related to aggressive forms of prostate cancer. The enzyme peptidylglycine alpha-amidating monooxygenase (PAM) is critical to the biosynthesis of amidated peptides and is commonly present in neuroendocrine cells. By northern blot hybridization analysis, PAM mRNA was detected in similar quantities in dorsolateral and ventral prostates of 3-month-old and 13-month-old rats. Multiple forms of PAM mRNA were present whose size distribution was more similar to PAM mRNAs found in pituitary than atrium. Alternative splice sites in PAM mRNA were investigated by reverse-transcriptase polymerase chain reaction. Similar alternatively spliced forms of PAM mRNA were found in both prostate lobes, pituitary, and atrium. However, the distribution of forms in the prostate most resembled that of pituitary. Multiple forms of PAM mRNA are present in prostate and may serve as markers of neuroendocrine differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 101-106 |
| Number of pages | 6 |
| Journal | Prostate |
| Volume | 29 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 1 1996 |
| Externally published | Yes |
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Keywords
- neuroendocrine peptides
- peptide amides
ASJC Scopus subject areas
- Urology
Cite this
Expression and processing of peptidylglycine alpha-amidating monooxygenase messenger RNA in rat prostate. / Samos, Luis F.; Gkonos, Peter J.
In: Prostate, Vol. 29, No. 2, 01.08.1996, p. 101-106.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression and processing of peptidylglycine alpha-amidating monooxygenase messenger RNA in rat prostate
AU - Samos, Luis F.
AU - Gkonos, Peter J.
PY - 1996/8/1
Y1 - 1996/8/1
N2 - The prostate gland contains neuroendocrine cells and amidated neuroendocrine peptides whose presence has been related to aggressive forms of prostate cancer. The enzyme peptidylglycine alpha-amidating monooxygenase (PAM) is critical to the biosynthesis of amidated peptides and is commonly present in neuroendocrine cells. By northern blot hybridization analysis, PAM mRNA was detected in similar quantities in dorsolateral and ventral prostates of 3-month-old and 13-month-old rats. Multiple forms of PAM mRNA were present whose size distribution was more similar to PAM mRNAs found in pituitary than atrium. Alternative splice sites in PAM mRNA were investigated by reverse-transcriptase polymerase chain reaction. Similar alternatively spliced forms of PAM mRNA were found in both prostate lobes, pituitary, and atrium. However, the distribution of forms in the prostate most resembled that of pituitary. Multiple forms of PAM mRNA are present in prostate and may serve as markers of neuroendocrine differentiation.
AB - The prostate gland contains neuroendocrine cells and amidated neuroendocrine peptides whose presence has been related to aggressive forms of prostate cancer. The enzyme peptidylglycine alpha-amidating monooxygenase (PAM) is critical to the biosynthesis of amidated peptides and is commonly present in neuroendocrine cells. By northern blot hybridization analysis, PAM mRNA was detected in similar quantities in dorsolateral and ventral prostates of 3-month-old and 13-month-old rats. Multiple forms of PAM mRNA were present whose size distribution was more similar to PAM mRNAs found in pituitary than atrium. Alternative splice sites in PAM mRNA were investigated by reverse-transcriptase polymerase chain reaction. Similar alternatively spliced forms of PAM mRNA were found in both prostate lobes, pituitary, and atrium. However, the distribution of forms in the prostate most resembled that of pituitary. Multiple forms of PAM mRNA are present in prostate and may serve as markers of neuroendocrine differentiation.
KW - neuroendocrine peptides
KW - peptide amides
UR - http://www.scopus.com/inward/record.url?scp=0029795978&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029795978&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0045(199608)29:2<101::AID-PROS5>3.0.CO;2-I
DO - 10.1002/(SICI)1097-0045(199608)29:2<101::AID-PROS5>3.0.CO;2-I
M3 - Article
C2 - 8700799
AN - SCOPUS:0029795978
VL - 29
SP - 101
EP - 106
JO - Prostate
JF - Prostate
SN - 0270-4137
IS - 2
ER -