Expression and possible implication of growth hormone-releasing hormone receptor splice variant 1 in endometriosis

Li Fu, Yutaka Osuga, Tetsu Yano, Yuri Takemura, Chieko Morimoto, Yasushi Hirota, Andrew V Schally, Yuji Taketani

Research output: Contribution to journalArticle

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Abstract

Objective: To determine possible involvement of splice variant 1 (SV1), a variant of the pituitary growth hormone-releasing hormone (GHRH) receptor, in the development of endometriosis. Design: Comparative and laboratory study. Setting: University teaching hospital reproductive endocrinology and infertility practice. Patient(s): Eutopic and ectopic endometrial tissues, and peritoneal bone marrow-derived cells were collected from women with or without endometriosis. Normal ovarian tissues were collected from women without endometriosis. Intervention(s): Ectopic endometrial stromal cells (ESC) were isolated and cultured with or without GHRH. Main Outcome Measure(s): Gene expression of GHRH and SV1 in the sample tissues was determined by reverse transcriptase (RT) nested polymerase chain reaction (PCR). Cyclic adenosine monophosphate (cAMP) production and 5-bromo-2'-deoxyuridine (BrdU) incorporation in ESC were measured using specific assay systems. Result(s): We detected SV1 messenger RNA (mRNA) in 17 out of 27 (63%) ectopic endometrial tissues, which was statistically significantly higher than that detected in eutopic endometrial tissues (2 out of 47, 4%) and normal ovarian tissues (0 out of 14). A relatively low rate of GHRH mRNA was detected in ectopic endometrial tissues (6 out of 27, 24%) and in eutopic endometrial tissues (12 out of 47, 26%). In contrast, relatively high rates were detected in normal ovarian tissues (14 out of 14, 100%) and peritoneal bone marrow-derived cells (13 out of 16, 81%). We found that GHRH stimulated the production of cAMP and the incorporation of BrdU in SV1-expressing ESC. Conclusion(s): GHRH and SV1 may play a role in promoting the development of endometriosis.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalFertility and Sterility
Volume92
Issue number1
DOIs
StatePublished - Jul 1 2009

Fingerprint

Endometriosis
Growth Hormone-Releasing Hormone
Choristoma
Stromal Cells
Bone Marrow Cells
Cyclic AMP
Pituitary Hormone-Regulating Hormone Receptors
Deoxyuridine
Messenger RNA
Endocrinology
Bromodeoxyuridine
Reverse Transcriptase Polymerase Chain Reaction
Teaching Hospitals
Infertility
Growth Hormone
somatotropin releasing hormone receptor
Outcome Assessment (Health Care)
Gene Expression
Polymerase Chain Reaction

Keywords

  • Endometriosis
  • GHRH
  • proliferation
  • SV1

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Expression and possible implication of growth hormone-releasing hormone receptor splice variant 1 in endometriosis. / Fu, Li; Osuga, Yutaka; Yano, Tetsu; Takemura, Yuri; Morimoto, Chieko; Hirota, Yasushi; Schally, Andrew V; Taketani, Yuji.

In: Fertility and Sterility, Vol. 92, No. 1, 01.07.2009, p. 47-53.

Research output: Contribution to journalArticle

Fu, Li ; Osuga, Yutaka ; Yano, Tetsu ; Takemura, Yuri ; Morimoto, Chieko ; Hirota, Yasushi ; Schally, Andrew V ; Taketani, Yuji. / Expression and possible implication of growth hormone-releasing hormone receptor splice variant 1 in endometriosis. In: Fertility and Sterility. 2009 ; Vol. 92, No. 1. pp. 47-53.
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abstract = "Objective: To determine possible involvement of splice variant 1 (SV1), a variant of the pituitary growth hormone-releasing hormone (GHRH) receptor, in the development of endometriosis. Design: Comparative and laboratory study. Setting: University teaching hospital reproductive endocrinology and infertility practice. Patient(s): Eutopic and ectopic endometrial tissues, and peritoneal bone marrow-derived cells were collected from women with or without endometriosis. Normal ovarian tissues were collected from women without endometriosis. Intervention(s): Ectopic endometrial stromal cells (ESC) were isolated and cultured with or without GHRH. Main Outcome Measure(s): Gene expression of GHRH and SV1 in the sample tissues was determined by reverse transcriptase (RT) nested polymerase chain reaction (PCR). Cyclic adenosine monophosphate (cAMP) production and 5-bromo-2'-deoxyuridine (BrdU) incorporation in ESC were measured using specific assay systems. Result(s): We detected SV1 messenger RNA (mRNA) in 17 out of 27 (63{\%}) ectopic endometrial tissues, which was statistically significantly higher than that detected in eutopic endometrial tissues (2 out of 47, 4{\%}) and normal ovarian tissues (0 out of 14). A relatively low rate of GHRH mRNA was detected in ectopic endometrial tissues (6 out of 27, 24{\%}) and in eutopic endometrial tissues (12 out of 47, 26{\%}). In contrast, relatively high rates were detected in normal ovarian tissues (14 out of 14, 100{\%}) and peritoneal bone marrow-derived cells (13 out of 16, 81{\%}). We found that GHRH stimulated the production of cAMP and the incorporation of BrdU in SV1-expressing ESC. Conclusion(s): GHRH and SV1 may play a role in promoting the development of endometriosis.",
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AU - Schally, Andrew V

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AB - Objective: To determine possible involvement of splice variant 1 (SV1), a variant of the pituitary growth hormone-releasing hormone (GHRH) receptor, in the development of endometriosis. Design: Comparative and laboratory study. Setting: University teaching hospital reproductive endocrinology and infertility practice. Patient(s): Eutopic and ectopic endometrial tissues, and peritoneal bone marrow-derived cells were collected from women with or without endometriosis. Normal ovarian tissues were collected from women without endometriosis. Intervention(s): Ectopic endometrial stromal cells (ESC) were isolated and cultured with or without GHRH. Main Outcome Measure(s): Gene expression of GHRH and SV1 in the sample tissues was determined by reverse transcriptase (RT) nested polymerase chain reaction (PCR). Cyclic adenosine monophosphate (cAMP) production and 5-bromo-2'-deoxyuridine (BrdU) incorporation in ESC were measured using specific assay systems. Result(s): We detected SV1 messenger RNA (mRNA) in 17 out of 27 (63%) ectopic endometrial tissues, which was statistically significantly higher than that detected in eutopic endometrial tissues (2 out of 47, 4%) and normal ovarian tissues (0 out of 14). A relatively low rate of GHRH mRNA was detected in ectopic endometrial tissues (6 out of 27, 24%) and in eutopic endometrial tissues (12 out of 47, 26%). In contrast, relatively high rates were detected in normal ovarian tissues (14 out of 14, 100%) and peritoneal bone marrow-derived cells (13 out of 16, 81%). We found that GHRH stimulated the production of cAMP and the incorporation of BrdU in SV1-expressing ESC. Conclusion(s): GHRH and SV1 may play a role in promoting the development of endometriosis.

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