Expression and functional analysis of murine ÇD30

S. Y. Nam, Richard K Lee, G. Miragliotta, E. R. Podack

Research output: Contribution to journalArticle

Abstract

CD30 expression and its possible signaling was studied in the murine system, focusing on lymphokine(LK) production. Balb/c lymph node(LN) cells activated with plate-bound anti-CD3 substantially expressed CD30 (15 and 60% after primary and secondary stimulation, respectively) and its expression was found on T cell, including both CD4+ and CDS+ cells, and non-T cell population. Activated LN cells in IL 4 (LN/IL 4) or IL 12 (LN/IL 12), which upregulated CD30 expression, were restimulated with anti-CD3 and/or antiCD30. Anti-CD30 alone showed minimal effect on LK production. However, by concurrent stimulation with anti-CD3, cross-linking of CD30 significantly upregulated Th2- type cytokine production of LN/IL 4, but not of LN/IL 12. These results indicate that CD30 may transduce crucial signal(s) in the polarized differentiation of Thl-Th2 cells depending on LK environment.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996

Fingerprint

Functional analysis
Lymphokines
lymphokines
Lymph Nodes
lymph nodes
Interleukin-12
Interleukin-4
mice
interleukin-12
interleukin-4
T-cells
cells
Th2 Cells
Cells
Cytokines
crosslinking
T-Lymphocytes
cytokines
T-lymphocytes
Population

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Nam, S. Y., Lee, R. K., Miragliotta, G., & Podack, E. R. (1996). Expression and functional analysis of murine ÇD30. FASEB Journal, 10(6).

Expression and functional analysis of murine ÇD30. / Nam, S. Y.; Lee, Richard K; Miragliotta, G.; Podack, E. R.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996.

Research output: Contribution to journalArticle

Nam, SY, Lee, RK, Miragliotta, G & Podack, ER 1996, 'Expression and functional analysis of murine ÇD30', FASEB Journal, vol. 10, no. 6.
Nam SY, Lee RK, Miragliotta G, Podack ER. Expression and functional analysis of murine ÇD30. FASEB Journal. 1996 Dec 1;10(6).
Nam, S. Y. ; Lee, Richard K ; Miragliotta, G. ; Podack, E. R. / Expression and functional analysis of murine ÇD30. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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