Abstract
CD30 expression and its possible signaling was studied in the murine system, focusing on lymphokine(LK) production. Balb/c lymph node(LN) cells activated with plate-bound anti-CD3 substantially expressed CD30 (15 and 60% after primary and secondary stimulation, respectively) and its expression was found on T cell, including both CD4+ and CDS+ cells, and non-T cell population. Activated LN cells in IL 4 (LN/IL 4) or IL 12 (LN/IL 12), which upregulated CD30 expression, were restimulated with anti-CD3 and/or antiCD30. Anti-CD30 alone showed minimal effect on LK production. However, by concurrent stimulation with anti-CD3, cross-linking of CD30 significantly upregulated Th2- type cytokine production of LN/IL 4, but not of LN/IL 12. These results indicate that CD30 may transduce crucial signal(s) in the polarized differentiation of Thl-Th2 cells depending on LK environment.
| Original language | English |
|---|---|
| Journal | FASEB Journal |
| Volume | 10 |
| Issue number | 6 |
| State | Published - Dec 1 1996 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
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Expression and functional analysis of murine ÇD30. / Nam, S. Y.; Lee, Richard K; Miragliotta, G.; Podack, E. R.
In: FASEB Journal, Vol. 10, No. 6, 01.12.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression and functional analysis of murine ÇD30
AU - Nam, S. Y.
AU - Lee, Richard K
AU - Miragliotta, G.
AU - Podack, E. R.
PY - 1996/12/1
Y1 - 1996/12/1
N2 - CD30 expression and its possible signaling was studied in the murine system, focusing on lymphokine(LK) production. Balb/c lymph node(LN) cells activated with plate-bound anti-CD3 substantially expressed CD30 (15 and 60% after primary and secondary stimulation, respectively) and its expression was found on T cell, including both CD4+ and CDS+ cells, and non-T cell population. Activated LN cells in IL 4 (LN/IL 4) or IL 12 (LN/IL 12), which upregulated CD30 expression, were restimulated with anti-CD3 and/or antiCD30. Anti-CD30 alone showed minimal effect on LK production. However, by concurrent stimulation with anti-CD3, cross-linking of CD30 significantly upregulated Th2- type cytokine production of LN/IL 4, but not of LN/IL 12. These results indicate that CD30 may transduce crucial signal(s) in the polarized differentiation of Thl-Th2 cells depending on LK environment.
AB - CD30 expression and its possible signaling was studied in the murine system, focusing on lymphokine(LK) production. Balb/c lymph node(LN) cells activated with plate-bound anti-CD3 substantially expressed CD30 (15 and 60% after primary and secondary stimulation, respectively) and its expression was found on T cell, including both CD4+ and CDS+ cells, and non-T cell population. Activated LN cells in IL 4 (LN/IL 4) or IL 12 (LN/IL 12), which upregulated CD30 expression, were restimulated with anti-CD3 and/or antiCD30. Anti-CD30 alone showed minimal effect on LK production. However, by concurrent stimulation with anti-CD3, cross-linking of CD30 significantly upregulated Th2- type cytokine production of LN/IL 4, but not of LN/IL 12. These results indicate that CD30 may transduce crucial signal(s) in the polarized differentiation of Thl-Th2 cells depending on LK environment.
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M3 - Article
AN - SCOPUS:33748913938
VL - 10
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 6
ER -