CD30 expression and its possible signaling was studied in the murine system, focusing on lymphokine(LK) production. Balb/c lymph node(LN) cells activated with plate-bound anti-CD3 substantially expressed CD30 (15 and 60% after primary and secondary stimulation, respectively) and its expression was found on T cell, including both CD4+ and CDS+ cells, and non-T cell population. Activated LN cells in IL 4 (LN/IL 4) or IL 12 (LN/IL 12), which upregulated CD30 expression, were restimulated with anti-CD3 and/or antiCD30. Anti-CD30 alone showed minimal effect on LK production. However, by concurrent stimulation with anti-CD3, cross-linking of CD30 significantly upregulated Th2- type cytokine production of LN/IL 4, but not of LN/IL 12. These results indicate that CD30 may transduce crucial signal(s) in the polarized differentiation of Thl-Th2 cells depending on LK environment.
|Original language||English (US)|
|State||Published - Dec 1 1996|
ASJC Scopus subject areas
- Molecular Biology