Expression and function of angiopoietin-1 in breast cancer

A. J. Hayes, W. Q. Huang, J. Yu, P. C. Maisonpierre, A. Liu, F. G. Kern, Marc E Lippman, S. W. McLeskey, L. Y. Li

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Angiopoietin-1 (Ang1) has been shown to act as an angiogenic promoter in embryonic angiogenesis by promoting vascular branching, pericyte recruitment and endothelial survival. We have investigated the role of Ang1 in tumour neovascularization under clinical conditions and in animal models. The expression of Ang1 in clinical breast cancer specimens was analysed by using laser-capture microdissection and reverse transcriptase-linked polymerase chain reaction (RT-PCR) on RNA isolated from the samples. Despite the expression of Ang1 in many human breast cancer cell lines, the gene was expressed in only three of 21 breast cancer clinical specimens, even though its receptor, Tie2, is abundant in the vasculature of all of these tumours. Ang1 was then overexpressed in a human breast cancer cell line (MCF-7) on its own and in conjunction with FGF1, an angiogenic factor shown to be able to increase the tumorigenicity of MCF-7 cells. High concentrations of Ang1 were produced in the conditioned media of the transfected cells (range 156-820 ng ml-1). However, in contrast to its physiological role as promoter of angiogenesis, overexpression of Ang1 did not enhance tumour growth, but instead caused up to a 3-fold retardation of tumour growth (P = 0.003). (C) 2000 Cancer Research Campaign.

Original languageEnglish
Pages (from-to)1154-1160
Number of pages7
JournalBritish Journal of Cancer
Volume83
Issue number9
StatePublished - Nov 15 2000
Externally publishedYes

Fingerprint

Angiopoietin-1
Breast Neoplasms
Neoplasms
TIE-2 Receptor
Laser Capture Microdissection
Fibroblast Growth Factor 1
Cell Line
Pericytes
Angiogenesis Inducing Agents
MCF-7 Cells
Conditioned Culture Medium
Growth
Reverse Transcriptase Polymerase Chain Reaction
Blood Vessels
Animal Models
RNA
Survival

Keywords

  • Angiogenesis
  • Angiopoietin
  • Breast cancer
  • Gene expression
  • Neovascularization
  • Tumorigenesis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hayes, A. J., Huang, W. Q., Yu, J., Maisonpierre, P. C., Liu, A., Kern, F. G., ... Li, L. Y. (2000). Expression and function of angiopoietin-1 in breast cancer. British Journal of Cancer, 83(9), 1154-1160.

Expression and function of angiopoietin-1 in breast cancer. / Hayes, A. J.; Huang, W. Q.; Yu, J.; Maisonpierre, P. C.; Liu, A.; Kern, F. G.; Lippman, Marc E; McLeskey, S. W.; Li, L. Y.

In: British Journal of Cancer, Vol. 83, No. 9, 15.11.2000, p. 1154-1160.

Research output: Contribution to journalArticle

Hayes, AJ, Huang, WQ, Yu, J, Maisonpierre, PC, Liu, A, Kern, FG, Lippman, ME, McLeskey, SW & Li, LY 2000, 'Expression and function of angiopoietin-1 in breast cancer', British Journal of Cancer, vol. 83, no. 9, pp. 1154-1160.
Hayes AJ, Huang WQ, Yu J, Maisonpierre PC, Liu A, Kern FG et al. Expression and function of angiopoietin-1 in breast cancer. British Journal of Cancer. 2000 Nov 15;83(9):1154-1160.
Hayes, A. J. ; Huang, W. Q. ; Yu, J. ; Maisonpierre, P. C. ; Liu, A. ; Kern, F. G. ; Lippman, Marc E ; McLeskey, S. W. ; Li, L. Y. / Expression and function of angiopoietin-1 in breast cancer. In: British Journal of Cancer. 2000 ; Vol. 83, No. 9. pp. 1154-1160.
@article{be452189774f4352bdd8c4c78eed3cf2,
title = "Expression and function of angiopoietin-1 in breast cancer",
abstract = "Angiopoietin-1 (Ang1) has been shown to act as an angiogenic promoter in embryonic angiogenesis by promoting vascular branching, pericyte recruitment and endothelial survival. We have investigated the role of Ang1 in tumour neovascularization under clinical conditions and in animal models. The expression of Ang1 in clinical breast cancer specimens was analysed by using laser-capture microdissection and reverse transcriptase-linked polymerase chain reaction (RT-PCR) on RNA isolated from the samples. Despite the expression of Ang1 in many human breast cancer cell lines, the gene was expressed in only three of 21 breast cancer clinical specimens, even though its receptor, Tie2, is abundant in the vasculature of all of these tumours. Ang1 was then overexpressed in a human breast cancer cell line (MCF-7) on its own and in conjunction with FGF1, an angiogenic factor shown to be able to increase the tumorigenicity of MCF-7 cells. High concentrations of Ang1 were produced in the conditioned media of the transfected cells (range 156-820 ng ml-1). However, in contrast to its physiological role as promoter of angiogenesis, overexpression of Ang1 did not enhance tumour growth, but instead caused up to a 3-fold retardation of tumour growth (P = 0.003). (C) 2000 Cancer Research Campaign.",
keywords = "Angiogenesis, Angiopoietin, Breast cancer, Gene expression, Neovascularization, Tumorigenesis",
author = "Hayes, {A. J.} and Huang, {W. Q.} and J. Yu and Maisonpierre, {P. C.} and A. Liu and Kern, {F. G.} and Lippman, {Marc E} and McLeskey, {S. W.} and Li, {L. Y.}",
year = "2000",
month = "11",
day = "15",
language = "English",
volume = "83",
pages = "1154--1160",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - Expression and function of angiopoietin-1 in breast cancer

AU - Hayes, A. J.

AU - Huang, W. Q.

AU - Yu, J.

AU - Maisonpierre, P. C.

AU - Liu, A.

AU - Kern, F. G.

AU - Lippman, Marc E

AU - McLeskey, S. W.

AU - Li, L. Y.

PY - 2000/11/15

Y1 - 2000/11/15

N2 - Angiopoietin-1 (Ang1) has been shown to act as an angiogenic promoter in embryonic angiogenesis by promoting vascular branching, pericyte recruitment and endothelial survival. We have investigated the role of Ang1 in tumour neovascularization under clinical conditions and in animal models. The expression of Ang1 in clinical breast cancer specimens was analysed by using laser-capture microdissection and reverse transcriptase-linked polymerase chain reaction (RT-PCR) on RNA isolated from the samples. Despite the expression of Ang1 in many human breast cancer cell lines, the gene was expressed in only three of 21 breast cancer clinical specimens, even though its receptor, Tie2, is abundant in the vasculature of all of these tumours. Ang1 was then overexpressed in a human breast cancer cell line (MCF-7) on its own and in conjunction with FGF1, an angiogenic factor shown to be able to increase the tumorigenicity of MCF-7 cells. High concentrations of Ang1 were produced in the conditioned media of the transfected cells (range 156-820 ng ml-1). However, in contrast to its physiological role as promoter of angiogenesis, overexpression of Ang1 did not enhance tumour growth, but instead caused up to a 3-fold retardation of tumour growth (P = 0.003). (C) 2000 Cancer Research Campaign.

AB - Angiopoietin-1 (Ang1) has been shown to act as an angiogenic promoter in embryonic angiogenesis by promoting vascular branching, pericyte recruitment and endothelial survival. We have investigated the role of Ang1 in tumour neovascularization under clinical conditions and in animal models. The expression of Ang1 in clinical breast cancer specimens was analysed by using laser-capture microdissection and reverse transcriptase-linked polymerase chain reaction (RT-PCR) on RNA isolated from the samples. Despite the expression of Ang1 in many human breast cancer cell lines, the gene was expressed in only three of 21 breast cancer clinical specimens, even though its receptor, Tie2, is abundant in the vasculature of all of these tumours. Ang1 was then overexpressed in a human breast cancer cell line (MCF-7) on its own and in conjunction with FGF1, an angiogenic factor shown to be able to increase the tumorigenicity of MCF-7 cells. High concentrations of Ang1 were produced in the conditioned media of the transfected cells (range 156-820 ng ml-1). However, in contrast to its physiological role as promoter of angiogenesis, overexpression of Ang1 did not enhance tumour growth, but instead caused up to a 3-fold retardation of tumour growth (P = 0.003). (C) 2000 Cancer Research Campaign.

KW - Angiogenesis

KW - Angiopoietin

KW - Breast cancer

KW - Gene expression

KW - Neovascularization

KW - Tumorigenesis

UR - http://www.scopus.com/inward/record.url?scp=0033753903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033753903&partnerID=8YFLogxK

M3 - Article

C2 - 11027428

AN - SCOPUS:0033753903

VL - 83

SP - 1154

EP - 1160

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 9

ER -