Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness

Gordon Broderick, Mary Ann Fletcher, Michael Gallagher, Zachary Barnes, Suzanne D. Vernon, Nancy G. Klimas

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Citations (Scopus)

Abstract

Complex disorders like Gulf War Illness (GWI) often defy diagnosis on the basis of a single biomarker and may only be distinguishable by considering the coexpression of multiple markers measured in response to a challenge. We demonstrate the practical application of such an approach using an example where blood was collected from 26 GWI, 13 healthy control subjects, and 9 unhealthy controls with Chronic Fatigue at three points during a graded exercise challenge. A 3-way multivariate projection model based on 12 markers of endocrine and immune function was constructed using a training set of n = 10 GWI and n = 11 healthy controls. These groups were separated almost completely on the basis of two coexpression patterns. In a separate test set these same features allowed for discrimination of new GWI subjects (n = 16) from unhealthy (n = 9) and healthy control subjects with a sensitivity of 70% and a specificity of 90%.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages145-164
Number of pages20
Volume934
DOIs
StatePublished - Nov 16 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume934
ISSN (Print)10643745

Fingerprint

Gulf War
Biomarkers
Healthy Volunteers
Fatigue
Exercise

Keywords

  • Batch partial least squares
  • Coexpression patterns
  • Cytokine profile
  • Diagnostic classification
  • Exercise response
  • Gulf War Illness
  • Partial least squares
  • Regression model

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Broderick, G., Fletcher, M. A., Gallagher, M., Barnes, Z., Vernon, S. D., & Klimas, N. G. (2012). Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness. In Methods in Molecular Biology (Vol. 934, pp. 145-164). (Methods in Molecular Biology; Vol. 934). https://doi.org/10.1007/978-1-62703-071-7_8

Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness. / Broderick, Gordon; Fletcher, Mary Ann; Gallagher, Michael; Barnes, Zachary; Vernon, Suzanne D.; Klimas, Nancy G.

Methods in Molecular Biology. Vol. 934 2012. p. 145-164 (Methods in Molecular Biology; Vol. 934).

Research output: Chapter in Book/Report/Conference proceedingChapter

Broderick, G, Fletcher, MA, Gallagher, M, Barnes, Z, Vernon, SD & Klimas, NG 2012, Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness. in Methods in Molecular Biology. vol. 934, Methods in Molecular Biology, vol. 934, pp. 145-164. https://doi.org/10.1007/978-1-62703-071-7_8
Broderick G, Fletcher MA, Gallagher M, Barnes Z, Vernon SD, Klimas NG. Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness. In Methods in Molecular Biology. Vol. 934. 2012. p. 145-164. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-071-7_8
Broderick, Gordon ; Fletcher, Mary Ann ; Gallagher, Michael ; Barnes, Zachary ; Vernon, Suzanne D. ; Klimas, Nancy G. / Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness. Methods in Molecular Biology. Vol. 934 2012. pp. 145-164 (Methods in Molecular Biology).
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