Abstract
Complex disorders like Gulf War Illness (GWI) often defy diagnosis on the basis of a single biomarker and may only be distinguishable by considering the coexpression of multiple markers measured in response to a challenge. We demonstrate the practical application of such an approach using an example where blood was collected from 26 GWI, 13 healthy control subjects, and 9 unhealthy controls with Chronic Fatigue at three points during a graded exercise challenge. A 3-way multivariate projection model based on 12 markers of endocrine and immune function was constructed using a training set of n = 10 GWI and n = 11 healthy controls. These groups were separated almost completely on the basis of two coexpression patterns. In a separate test set these same features allowed for discrimination of new GWI subjects (n = 16) from unhealthy (n = 9) and healthy control subjects with a sensitivity of 70% and a specificity of 90%.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Pages | 145-164 |
| Number of pages | 20 |
| Volume | 934 |
| DOIs | |
| State | Published - Nov 16 2012 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 934 |
| ISSN (Print) | 10643745 |
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Keywords
- Batch partial least squares
- Coexpression patterns
- Cytokine profile
- Diagnostic classification
- Exercise response
- Gulf War Illness
- Partial least squares
- Regression model
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness. / Broderick, Gordon; Fletcher, Mary Ann; Gallagher, Michael; Barnes, Zachary; Vernon, Suzanne D.; Klimas, Nancy G.
Methods in Molecular Biology. Vol. 934 2012. p. 145-164 (Methods in Molecular Biology; Vol. 934).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness
AU - Broderick, Gordon
AU - Fletcher, Mary Ann
AU - Gallagher, Michael
AU - Barnes, Zachary
AU - Vernon, Suzanne D.
AU - Klimas, Nancy G.
PY - 2012/11/16
Y1 - 2012/11/16
N2 - Complex disorders like Gulf War Illness (GWI) often defy diagnosis on the basis of a single biomarker and may only be distinguishable by considering the coexpression of multiple markers measured in response to a challenge. We demonstrate the practical application of such an approach using an example where blood was collected from 26 GWI, 13 healthy control subjects, and 9 unhealthy controls with Chronic Fatigue at three points during a graded exercise challenge. A 3-way multivariate projection model based on 12 markers of endocrine and immune function was constructed using a training set of n = 10 GWI and n = 11 healthy controls. These groups were separated almost completely on the basis of two coexpression patterns. In a separate test set these same features allowed for discrimination of new GWI subjects (n = 16) from unhealthy (n = 9) and healthy control subjects with a sensitivity of 70% and a specificity of 90%.
AB - Complex disorders like Gulf War Illness (GWI) often defy diagnosis on the basis of a single biomarker and may only be distinguishable by considering the coexpression of multiple markers measured in response to a challenge. We demonstrate the practical application of such an approach using an example where blood was collected from 26 GWI, 13 healthy control subjects, and 9 unhealthy controls with Chronic Fatigue at three points during a graded exercise challenge. A 3-way multivariate projection model based on 12 markers of endocrine and immune function was constructed using a training set of n = 10 GWI and n = 11 healthy controls. These groups were separated almost completely on the basis of two coexpression patterns. In a separate test set these same features allowed for discrimination of new GWI subjects (n = 16) from unhealthy (n = 9) and healthy control subjects with a sensitivity of 70% and a specificity of 90%.
KW - Batch partial least squares
KW - Coexpression patterns
KW - Cytokine profile
KW - Diagnostic classification
KW - Exercise response
KW - Gulf War Illness
KW - Partial least squares
KW - Regression model
UR - http://www.scopus.com/inward/record.url?scp=84868681298&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84868681298&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-071-7_8
DO - 10.1007/978-1-62703-071-7_8
M3 - Chapter
SN - 9781627030700
VL - 934
T3 - Methods in Molecular Biology
SP - 145
EP - 164
BT - Methods in Molecular Biology
ER -