Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness

Gordon Broderick; Mary Ann Fletcher; Michael Gallagher; Zachary Barnes; Suzanne D. Vernon; Nancy G. Klimas

doi:10.1007/978-1-62703-071-7_8

Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness

Gordon Broderick, Mary Ann Fletcher, Michael Gallagher, Zachary Barnes, Suzanne D. Vernon, Nancy G. Klimas

Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

12 Scopus citations

Abstract

Complex disorders like Gulf War Illness (GWI) often defy diagnosis on the basis of a single biomarker and may only be distinguishable by considering the coexpression of multiple markers measured in response to a challenge. We demonstrate the practical application of such an approach using an example where blood was collected from 26 GWI, 13 healthy control subjects, and 9 unhealthy controls with Chronic Fatigue at three points during a graded exercise challenge. A 3-way multivariate projection model based on 12 markers of endocrine and immune function was constructed using a training set of n = 10 GWI and n = 11 healthy controls. These groups were separated almost completely on the basis of two coexpression patterns. In a separate test set these same features allowed for discrimination of new GWI subjects (n = 16) from unhealthy (n = 9) and healthy control subjects with a sensitivity of 70% and a specificity of 90%.

Original language	English (US)
Title of host publication	Psychoneuroimmunology
Subtitle of host publication	Methods and Protocols
Editors	Qing Yan, Qing Yan
Pages	145-164
Number of pages	20
DOIs	https://doi.org/10.1007/978-1-62703-071-7_8
State	Published - 2012

Publication series

Name	Methods in Molecular Biology
Volume	934
ISSN (Print)	1064-3745

Keywords

Batch partial least squares
Coexpression patterns
Cytokine profile
Diagnostic classification
Exercise response
Gulf War Illness
Partial least squares
Regression model

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-62703-071-7_8

Cite this

Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness. / Broderick, Gordon; Fletcher, Mary Ann; Gallagher, Michael; Barnes, Zachary; Vernon, Suzanne D.; Klimas, Nancy G.

Psychoneuroimmunology: Methods and Protocols. ed. / Qing Yan; Qing Yan. 2012. p. 145-164 (Methods in Molecular Biology; Vol. 934).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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Exploring the diagnostic potential of immune biomarker coexpression in gulf war illness

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Keywords

ASJC Scopus subject areas

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