Exploring protein-protein interactions involving newly synthesized mitochondrial dna-encoded proteins

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Biogenesis of the mitochondrial respiratory chain enzymes involves the coordinated action of the mitochondrial and nuclear genomes. As a matter of fact, the structural subunits forming these multimeric enzymes are encoded in both genomes. In addition, the assistance of nuclear encoded factors, termed assembly factors, is necessary to allow for the expression of the mitochondrial DNA-encoded subunits and to facilitate their maturation, membrane insertion, and further assembly into the corresponding enzymatic complex. These processes involve transient interactions among the newly synthesized mitochondrial products and specific assembly factors. The identification and characterization of these interactions can be achieved by the method described here, consisting of pulling down tagged versions of the assembly factors immediately after radiolabeling the mitochondrial translation products in isolated mitochondria, and analyzing the radiolabeled pulled-down material.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages125-139
Number of pages15
Volume457
DOIs
StatePublished - Dec 1 2008

Publication series

NameMethods in Molecular Biology
Volume457
ISSN (Print)10643745

Keywords

  • GST pull-down
  • In organello protein synthesis
  • Mitochondria; mitochondrial biogenesis
  • Mitochondrial translation
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Exploring protein-protein interactions involving newly synthesized mitochondrial dna-encoded proteins'. Together they form a unique fingerprint.

  • Cite this

    Horn, D., Fontanesi, F., & Barrientos, A. (2008). Exploring protein-protein interactions involving newly synthesized mitochondrial dna-encoded proteins. In Methods in Molecular Biology (Vol. 457, pp. 125-139). (Methods in Molecular Biology; Vol. 457). https://doi.org/10.1007/978-1-59745-261-8-9