Exploring protein-protein interactions involving newly synthesized mitochondrial dna-encoded proteins

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Biogenesis of the mitochondrial respiratory chain enzymes involves the coordinated action of the mitochondrial and nuclear genomes. As a matter of fact, the structural subunits forming these multimeric enzymes are encoded in both genomes. In addition, the assistance of nuclear encoded factors, termed assembly factors, is necessary to allow for the expression of the mitochondrial DNA-encoded subunits and to facilitate their maturation, membrane insertion, and further assembly into the corresponding enzymatic complex. These processes involve transient interactions among the newly synthesized mitochondrial products and specific assembly factors. The identification and characterization of these interactions can be achieved by the method described here, consisting of pulling down tagged versions of the assembly factors immediately after radiolabeling the mitochondrial translation products in isolated mitochondria, and analyzing the radiolabeled pulled-down material.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages125-139
Number of pages15
Volume457
ISBN (Print)9781588299253
DOIs
StatePublished - 2008

Publication series

NameMethods in Molecular Biology
Volume457
ISSN (Print)10643745

Fingerprint

Mitochondrial Genome
Organelle Biogenesis
Enzymes
Electron Transport
Mitochondrial DNA
Mitochondria
Proteins
Genome
Membranes

Keywords

  • GST pull-down
  • In organello protein synthesis
  • Mitochondria; mitochondrial biogenesis
  • Mitochondrial translation
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Horn, D., Fontanesi, F., & Barrientos, A. (2008). Exploring protein-protein interactions involving newly synthesized mitochondrial dna-encoded proteins. In Methods in Molecular Biology (Vol. 457, pp. 125-139). (Methods in Molecular Biology; Vol. 457). Humana Press Inc.. https://doi.org/10.1007/978-1-59745-261-8_9

Exploring protein-protein interactions involving newly synthesized mitochondrial dna-encoded proteins. / Horn, Darryl; Fontanesi, Flavia; Barrientos, Antonio.

Methods in Molecular Biology. Vol. 457 Humana Press Inc., 2008. p. 125-139 (Methods in Molecular Biology; Vol. 457).

Research output: Chapter in Book/Report/Conference proceedingChapter

Horn, D, Fontanesi, F & Barrientos, A 2008, Exploring protein-protein interactions involving newly synthesized mitochondrial dna-encoded proteins. in Methods in Molecular Biology. vol. 457, Methods in Molecular Biology, vol. 457, Humana Press Inc., pp. 125-139. https://doi.org/10.1007/978-1-59745-261-8_9
Horn D, Fontanesi F, Barrientos A. Exploring protein-protein interactions involving newly synthesized mitochondrial dna-encoded proteins. In Methods in Molecular Biology. Vol. 457. Humana Press Inc. 2008. p. 125-139. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-261-8_9
Horn, Darryl ; Fontanesi, Flavia ; Barrientos, Antonio. / Exploring protein-protein interactions involving newly synthesized mitochondrial dna-encoded proteins. Methods in Molecular Biology. Vol. 457 Humana Press Inc., 2008. pp. 125-139 (Methods in Molecular Biology).
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