Exploring bioanalytical applications of assisted protein reassembly

Research output: Contribution to journalReview article

4 Scopus citations

Abstract

Reassembly of protein from its peptide fragments is a technique that can have many applications in the bioanalytical field. Typically, a reporter protein fragmented into its two peptides is employed as a label in this study. This fragments of peptide can reassemble yielding an active functional reporter. This reassembly of the protein can be assisted by non-covalently interacting peptides or proteins, which are attached to the fragmented reporter. This technique has been employed in several applications including study of protein-protein interactions, antibody screening, immunoassays, and high-throughput screening. This review focuses on different reporters employed in the study of reassembly of proteins and applications of this strategy in bioanalysis.

Original languageEnglish (US)
Pages (from-to)383-390
Number of pages8
JournalAnalytical and bioanalytical chemistry
Volume379
Issue number3
DOIs
StatePublished - Jun 1 2004
Externally publishedYes

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Keywords

  • Assisted protein reassembly
  • Immunoassasy
  • Molecular rulers
  • Protein-protein interactions
  • Reporter protein

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

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