Exploiting post-mitotic yeast cultures to model neurodegeneration

Andrea Ruetenik, Antonio Barrientos

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

Over the last few decades, the budding yeast Saccharomyces cerevisiae has been extensively used as a valuable organism to explore mechanisms of aging and human age-associated neurodegenerative disorders. Yeast models can be used to study loss of function of disease-related conserved genes and to investigate gain of function activities, frequently proteotoxicity, exerted by non-conserved human mutant proteins responsible for neurodegeneration. Most published models of proteotoxicity have used rapidly dividing cells and suffer from a high level of protein expression resulting in acute growth arrest or cell death. This contrasts with the slow development of neurodegenerative proteotoxicity during aging and the characteristic post-mitotic state of the affected cell type, the neuron. Here, we will review the efforts to create and characterize yeast models of neurodegeneration using the chronological life span model of aging, and the specific information they can provide regarding the chronology of physiological events leading to neurotoxic proteotoxicity-induced cell death and the identification of new pathways involved.

Original languageEnglish (US)
Article number400
JournalFrontiers in Molecular Neuroscience
Volume11
DOIs
StatePublished - Nov 2 2018

Fingerprint

Cell Death
Yeasts
Chronology
Saccharomycetales
Mutant Proteins
Neurodegenerative Diseases
Saccharomyces cerevisiae
Neurons
Growth
Genes
Proteins

Keywords

  • Chronological life span
  • Inducible yeast model
  • Mitochondria
  • Neurodegenerative disorder
  • Proteotoxicity
  • Reactive oxygen species
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Exploiting post-mitotic yeast cultures to model neurodegeneration. / Ruetenik, Andrea; Barrientos, Antonio.

In: Frontiers in Molecular Neuroscience, Vol. 11, 400, 02.11.2018.

Research output: Contribution to journalReview article

@article{138407b36e644f9f9b590d19dc22e0ed,
title = "Exploiting post-mitotic yeast cultures to model neurodegeneration",
abstract = "Over the last few decades, the budding yeast Saccharomyces cerevisiae has been extensively used as a valuable organism to explore mechanisms of aging and human age-associated neurodegenerative disorders. Yeast models can be used to study loss of function of disease-related conserved genes and to investigate gain of function activities, frequently proteotoxicity, exerted by non-conserved human mutant proteins responsible for neurodegeneration. Most published models of proteotoxicity have used rapidly dividing cells and suffer from a high level of protein expression resulting in acute growth arrest or cell death. This contrasts with the slow development of neurodegenerative proteotoxicity during aging and the characteristic post-mitotic state of the affected cell type, the neuron. Here, we will review the efforts to create and characterize yeast models of neurodegeneration using the chronological life span model of aging, and the specific information they can provide regarding the chronology of physiological events leading to neurotoxic proteotoxicity-induced cell death and the identification of new pathways involved.",
keywords = "Chronological life span, Inducible yeast model, Mitochondria, Neurodegenerative disorder, Proteotoxicity, Reactive oxygen species, Saccharomyces cerevisiae",
author = "Andrea Ruetenik and Antonio Barrientos",
year = "2018",
month = "11",
day = "2",
doi = "10.3389/fnmol.2018.00400",
language = "English (US)",
volume = "11",
journal = "Frontiers in Molecular Neuroscience",
issn = "1662-5099",
publisher = "Frontiers Research Foundation",

}

TY - JOUR

T1 - Exploiting post-mitotic yeast cultures to model neurodegeneration

AU - Ruetenik, Andrea

AU - Barrientos, Antonio

PY - 2018/11/2

Y1 - 2018/11/2

N2 - Over the last few decades, the budding yeast Saccharomyces cerevisiae has been extensively used as a valuable organism to explore mechanisms of aging and human age-associated neurodegenerative disorders. Yeast models can be used to study loss of function of disease-related conserved genes and to investigate gain of function activities, frequently proteotoxicity, exerted by non-conserved human mutant proteins responsible for neurodegeneration. Most published models of proteotoxicity have used rapidly dividing cells and suffer from a high level of protein expression resulting in acute growth arrest or cell death. This contrasts with the slow development of neurodegenerative proteotoxicity during aging and the characteristic post-mitotic state of the affected cell type, the neuron. Here, we will review the efforts to create and characterize yeast models of neurodegeneration using the chronological life span model of aging, and the specific information they can provide regarding the chronology of physiological events leading to neurotoxic proteotoxicity-induced cell death and the identification of new pathways involved.

AB - Over the last few decades, the budding yeast Saccharomyces cerevisiae has been extensively used as a valuable organism to explore mechanisms of aging and human age-associated neurodegenerative disorders. Yeast models can be used to study loss of function of disease-related conserved genes and to investigate gain of function activities, frequently proteotoxicity, exerted by non-conserved human mutant proteins responsible for neurodegeneration. Most published models of proteotoxicity have used rapidly dividing cells and suffer from a high level of protein expression resulting in acute growth arrest or cell death. This contrasts with the slow development of neurodegenerative proteotoxicity during aging and the characteristic post-mitotic state of the affected cell type, the neuron. Here, we will review the efforts to create and characterize yeast models of neurodegeneration using the chronological life span model of aging, and the specific information they can provide regarding the chronology of physiological events leading to neurotoxic proteotoxicity-induced cell death and the identification of new pathways involved.

KW - Chronological life span

KW - Inducible yeast model

KW - Mitochondria

KW - Neurodegenerative disorder

KW - Proteotoxicity

KW - Reactive oxygen species

KW - Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=85056714653&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056714653&partnerID=8YFLogxK

U2 - 10.3389/fnmol.2018.00400

DO - 10.3389/fnmol.2018.00400

M3 - Review article

AN - SCOPUS:85056714653

VL - 11

JO - Frontiers in Molecular Neuroscience

JF - Frontiers in Molecular Neuroscience

SN - 1662-5099

M1 - 400

ER -