Experimental studies on the ultrastructural localization of acetylcholinesterase in the mediobasal hypothalamus of the rat

K. A. Carson, Charles Nemeroff, M. S. Rone, G. F. Nicholson, J. S. Kizer, J. S. Hanker

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Abstract

Acetylcholinesterase (AChE) activity has been demonstrated ultrastructurally in neurons of the arcuate nucleus and associated with fibers in the arcuate nucleus neuropil and the median eminence (ME) of the rat. In addition, the effects of neonatal monosodium glutamate (MSG) treatment and Halasz deafferentation on the AChE staining and localization have been studied. Neonatal MSG-treatment resulted in loss of the majority of AChE positive neurons in the arcuate nucleus while leaving neuropil staining intact. Halasz deafferentation caused a loss of arcuate neuropil activity while leaving the neuronal staining unaltered. These observations are consistent with previous biochemical results suggesting the existence of a cholinergic tuberoinfundibular system with nerve cells in the arcuate nucleus and terminals in the median eminence. In addition, the deafferentation experiments indicated that extra hypothalamic cholinergic fibers may innervate the arcuate nucleus. Supporting evidence from other biochemical studies and the curious paucity of histochemical and biochemical AChE activity in the ME are also discussed.

Original languageEnglish
Pages (from-to)201-219
Number of pages19
JournalJournal of Comparative Neurology
Volume182
Issue number2
StatePublished - Dec 1 1978
Externally publishedYes

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Arcuate Nucleus of Hypothalamus
Acetylcholinesterase
Hypothalamus
Median Eminence
Neuropil
Sodium Glutamate
Staining and Labeling
Neurons
Cholinergic Fibers
Cholinergic Agents

ASJC Scopus subject areas

  • Neuroscience(all)

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Experimental studies on the ultrastructural localization of acetylcholinesterase in the mediobasal hypothalamus of the rat. / Carson, K. A.; Nemeroff, Charles; Rone, M. S.; Nicholson, G. F.; Kizer, J. S.; Hanker, J. S.

In: Journal of Comparative Neurology, Vol. 182, No. 2, 01.12.1978, p. 201-219.

Research output: Contribution to journalArticle

Carson, K. A. ; Nemeroff, Charles ; Rone, M. S. ; Nicholson, G. F. ; Kizer, J. S. ; Hanker, J. S. / Experimental studies on the ultrastructural localization of acetylcholinesterase in the mediobasal hypothalamus of the rat. In: Journal of Comparative Neurology. 1978 ; Vol. 182, No. 2. pp. 201-219.
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