Experimental ocular tuberculosis in guinea pigs

Narsing A. Rao, Thomas A Albini, Mirnalini Kumaradas, Michael L. Pinn, Mostafa M. Fraig, Petros C. Karakousis

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objective: To develop an animal model of intraocular tuberculosis (TB) with features of pulmonary TB and extrapulmonary dissemination to the eye. Methods: Hartley strain guinea pigs were infected via an aerosol route with virulent Mycobacterium tuberculosis. One group of guinea pigs was infected with a relatively low bacterial inoculum and received no treatment. A second group of guinea pigs received high-dose infection and were treated with the first-line anti-TB drugs isoniazid, rifampin, and pyrazinamide. Development of ocular TB lesions was documented by histological analysis, acid-fast staining, and real-time polymerase chain reaction for M tuberculosis DNA. Results: Untreated guinea pigs developed pulmonary and extrapulmonary TB. Ocular TB, primarily involving the uvea, developed in 5 of 12 eyes (42%). Uveal granulomatous lesions showed the presence of acid-fast organisms and M tuberculosis DNA. In treated animals, none of the 8 eyes examined revealed the presence of acidfast organisms; however, there was mild nongranulomatous uveitis in 4 eyes. Conclusions: Mycobacterium tuberculosis delivered via aerosol to guinea pigs results in extrapulmonary dissemination to the eye. Of significance, intraocular changes in this model include granulomatous inflammation and the presence of acid-fast organisms, as seen in human cases of ocular TB. Clinical Relevance: The guinea pig model may provide greater insight into the pathogenesis of intraocular TB and assist in the development of novel modalities to treat this global infectious disease.

Original languageEnglish
Pages (from-to)1162-1166
Number of pages5
JournalArchives of Ophthalmology
Volume127
Issue number9
DOIs
StatePublished - Sep 1 2009

Fingerprint

Ocular Tuberculosis
Guinea Pigs
Tuberculosis
Aerosols
Pulmonary Tuberculosis
Mycobacterium tuberculosis
Acids
Uvea
Pyrazinamide
DNA
Uveitis
Isoniazid
Rifampin
Communicable Diseases
Real-Time Polymerase Chain Reaction
Animal Models
Staining and Labeling
Inflammation
Infection

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Rao, N. A., Albini, T. A., Kumaradas, M., Pinn, M. L., Fraig, M. M., & Karakousis, P. C. (2009). Experimental ocular tuberculosis in guinea pigs. Archives of Ophthalmology, 127(9), 1162-1166. https://doi.org/10.1001/archophthalmol.2009.220

Experimental ocular tuberculosis in guinea pigs. / Rao, Narsing A.; Albini, Thomas A; Kumaradas, Mirnalini; Pinn, Michael L.; Fraig, Mostafa M.; Karakousis, Petros C.

In: Archives of Ophthalmology, Vol. 127, No. 9, 01.09.2009, p. 1162-1166.

Research output: Contribution to journalArticle

Rao, NA, Albini, TA, Kumaradas, M, Pinn, ML, Fraig, MM & Karakousis, PC 2009, 'Experimental ocular tuberculosis in guinea pigs', Archives of Ophthalmology, vol. 127, no. 9, pp. 1162-1166. https://doi.org/10.1001/archophthalmol.2009.220
Rao NA, Albini TA, Kumaradas M, Pinn ML, Fraig MM, Karakousis PC. Experimental ocular tuberculosis in guinea pigs. Archives of Ophthalmology. 2009 Sep 1;127(9):1162-1166. https://doi.org/10.1001/archophthalmol.2009.220
Rao, Narsing A. ; Albini, Thomas A ; Kumaradas, Mirnalini ; Pinn, Michael L. ; Fraig, Mostafa M. ; Karakousis, Petros C. / Experimental ocular tuberculosis in guinea pigs. In: Archives of Ophthalmology. 2009 ; Vol. 127, No. 9. pp. 1162-1166.
@article{d8e5239f7a274af3abf6d6d36334eea8,
title = "Experimental ocular tuberculosis in guinea pigs",
abstract = "Objective: To develop an animal model of intraocular tuberculosis (TB) with features of pulmonary TB and extrapulmonary dissemination to the eye. Methods: Hartley strain guinea pigs were infected via an aerosol route with virulent Mycobacterium tuberculosis. One group of guinea pigs was infected with a relatively low bacterial inoculum and received no treatment. A second group of guinea pigs received high-dose infection and were treated with the first-line anti-TB drugs isoniazid, rifampin, and pyrazinamide. Development of ocular TB lesions was documented by histological analysis, acid-fast staining, and real-time polymerase chain reaction for M tuberculosis DNA. Results: Untreated guinea pigs developed pulmonary and extrapulmonary TB. Ocular TB, primarily involving the uvea, developed in 5 of 12 eyes (42{\%}). Uveal granulomatous lesions showed the presence of acid-fast organisms and M tuberculosis DNA. In treated animals, none of the 8 eyes examined revealed the presence of acidfast organisms; however, there was mild nongranulomatous uveitis in 4 eyes. Conclusions: Mycobacterium tuberculosis delivered via aerosol to guinea pigs results in extrapulmonary dissemination to the eye. Of significance, intraocular changes in this model include granulomatous inflammation and the presence of acid-fast organisms, as seen in human cases of ocular TB. Clinical Relevance: The guinea pig model may provide greater insight into the pathogenesis of intraocular TB and assist in the development of novel modalities to treat this global infectious disease.",
author = "Rao, {Narsing A.} and Albini, {Thomas A} and Mirnalini Kumaradas and Pinn, {Michael L.} and Fraig, {Mostafa M.} and Karakousis, {Petros C.}",
year = "2009",
month = "9",
day = "1",
doi = "10.1001/archophthalmol.2009.220",
language = "English",
volume = "127",
pages = "1162--1166",
journal = "JAMA Ophthalmology",
issn = "2168-6165",
publisher = "American Medical Association",
number = "9",

}

TY - JOUR

T1 - Experimental ocular tuberculosis in guinea pigs

AU - Rao, Narsing A.

AU - Albini, Thomas A

AU - Kumaradas, Mirnalini

AU - Pinn, Michael L.

AU - Fraig, Mostafa M.

AU - Karakousis, Petros C.

PY - 2009/9/1

Y1 - 2009/9/1

N2 - Objective: To develop an animal model of intraocular tuberculosis (TB) with features of pulmonary TB and extrapulmonary dissemination to the eye. Methods: Hartley strain guinea pigs were infected via an aerosol route with virulent Mycobacterium tuberculosis. One group of guinea pigs was infected with a relatively low bacterial inoculum and received no treatment. A second group of guinea pigs received high-dose infection and were treated with the first-line anti-TB drugs isoniazid, rifampin, and pyrazinamide. Development of ocular TB lesions was documented by histological analysis, acid-fast staining, and real-time polymerase chain reaction for M tuberculosis DNA. Results: Untreated guinea pigs developed pulmonary and extrapulmonary TB. Ocular TB, primarily involving the uvea, developed in 5 of 12 eyes (42%). Uveal granulomatous lesions showed the presence of acid-fast organisms and M tuberculosis DNA. In treated animals, none of the 8 eyes examined revealed the presence of acidfast organisms; however, there was mild nongranulomatous uveitis in 4 eyes. Conclusions: Mycobacterium tuberculosis delivered via aerosol to guinea pigs results in extrapulmonary dissemination to the eye. Of significance, intraocular changes in this model include granulomatous inflammation and the presence of acid-fast organisms, as seen in human cases of ocular TB. Clinical Relevance: The guinea pig model may provide greater insight into the pathogenesis of intraocular TB and assist in the development of novel modalities to treat this global infectious disease.

AB - Objective: To develop an animal model of intraocular tuberculosis (TB) with features of pulmonary TB and extrapulmonary dissemination to the eye. Methods: Hartley strain guinea pigs were infected via an aerosol route with virulent Mycobacterium tuberculosis. One group of guinea pigs was infected with a relatively low bacterial inoculum and received no treatment. A second group of guinea pigs received high-dose infection and were treated with the first-line anti-TB drugs isoniazid, rifampin, and pyrazinamide. Development of ocular TB lesions was documented by histological analysis, acid-fast staining, and real-time polymerase chain reaction for M tuberculosis DNA. Results: Untreated guinea pigs developed pulmonary and extrapulmonary TB. Ocular TB, primarily involving the uvea, developed in 5 of 12 eyes (42%). Uveal granulomatous lesions showed the presence of acid-fast organisms and M tuberculosis DNA. In treated animals, none of the 8 eyes examined revealed the presence of acidfast organisms; however, there was mild nongranulomatous uveitis in 4 eyes. Conclusions: Mycobacterium tuberculosis delivered via aerosol to guinea pigs results in extrapulmonary dissemination to the eye. Of significance, intraocular changes in this model include granulomatous inflammation and the presence of acid-fast organisms, as seen in human cases of ocular TB. Clinical Relevance: The guinea pig model may provide greater insight into the pathogenesis of intraocular TB and assist in the development of novel modalities to treat this global infectious disease.

UR - http://www.scopus.com/inward/record.url?scp=70349255601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349255601&partnerID=8YFLogxK

U2 - 10.1001/archophthalmol.2009.220

DO - 10.1001/archophthalmol.2009.220

M3 - Article

C2 - 19752425

AN - SCOPUS:70349255601

VL - 127

SP - 1162

EP - 1166

JO - JAMA Ophthalmology

JF - JAMA Ophthalmology

SN - 2168-6165

IS - 9

ER -