Abstract
The human motilin gene has been isolated and characterized. The gene spans about 9 kilobase pairs (kb) and the 0.7 kb motilin mRNA is encoded by five exons. The 22-amino-acid motilin sequence is encoded by exons 2 and 3. The human motilin gene was mapped to the p21.2→p21.3 region of chromosome 6 by hybridization of the cloned cDNA to DNAs from a panel of reduced human-mouse somatic cell hybrids and by in situ hybridization to human prometaphase chromosomes. RNA blotting using RNA prepared from various regions of the human gastrointestinal tract revealed high levels of motilin mRNA in duodenum and lower levels in the antrum of the stomach; motilin mRNA could not be detected by this procedure in the esophagus, cardia of the stomach, descending colon or gallbladder.
| Original language | English |
|---|---|
| Pages (from-to) | 248-252 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 249 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 5 1989 |
| Externally published | Yes |
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Keywords
- (Duodenum, Stomach, Human)
- Chromosome 6
- Motilin gene
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Exon-intron organization, expression, and chromosomal localization of the human motilin gene. / Yano, H.; Seino, Y.; Fujita, J.; Yamada, Y.; Inagaki, N.; Takeda, J.; Bell, G. I.; Eddy, R. L.; Fan, Yao-Shan; Byers, M. G.; Shows, T. B.; Imura, H.
In: FEBS Letters, Vol. 249, No. 2, 05.06.1989, p. 248-252.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Exon-intron organization, expression, and chromosomal localization of the human motilin gene
AU - Yano, H.
AU - Seino, Y.
AU - Fujita, J.
AU - Yamada, Y.
AU - Inagaki, N.
AU - Takeda, J.
AU - Bell, G. I.
AU - Eddy, R. L.
AU - Fan, Yao-Shan
AU - Byers, M. G.
AU - Shows, T. B.
AU - Imura, H.
PY - 1989/6/5
Y1 - 1989/6/5
N2 - The human motilin gene has been isolated and characterized. The gene spans about 9 kilobase pairs (kb) and the 0.7 kb motilin mRNA is encoded by five exons. The 22-amino-acid motilin sequence is encoded by exons 2 and 3. The human motilin gene was mapped to the p21.2→p21.3 region of chromosome 6 by hybridization of the cloned cDNA to DNAs from a panel of reduced human-mouse somatic cell hybrids and by in situ hybridization to human prometaphase chromosomes. RNA blotting using RNA prepared from various regions of the human gastrointestinal tract revealed high levels of motilin mRNA in duodenum and lower levels in the antrum of the stomach; motilin mRNA could not be detected by this procedure in the esophagus, cardia of the stomach, descending colon or gallbladder.
AB - The human motilin gene has been isolated and characterized. The gene spans about 9 kilobase pairs (kb) and the 0.7 kb motilin mRNA is encoded by five exons. The 22-amino-acid motilin sequence is encoded by exons 2 and 3. The human motilin gene was mapped to the p21.2→p21.3 region of chromosome 6 by hybridization of the cloned cDNA to DNAs from a panel of reduced human-mouse somatic cell hybrids and by in situ hybridization to human prometaphase chromosomes. RNA blotting using RNA prepared from various regions of the human gastrointestinal tract revealed high levels of motilin mRNA in duodenum and lower levels in the antrum of the stomach; motilin mRNA could not be detected by this procedure in the esophagus, cardia of the stomach, descending colon or gallbladder.
KW - (Duodenum, Stomach, Human)
KW - Chromosome 6
KW - Motilin gene
UR - http://www.scopus.com/inward/record.url?scp=0024309893&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024309893&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(89)80633-7
DO - 10.1016/0014-5793(89)80633-7
M3 - Article
C2 - 2737284
AN - SCOPUS:0024309893
VL - 249
SP - 248
EP - 252
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -