Exon-intron organization, expression, and chromosomal localization of the human motilin gene

H. Yano, Y. Seino, J. Fujita, Y. Yamada, N. Inagaki, J. Takeda, G. I. Bell, R. L. Eddy, Y. S. Fan, M. G. Byers, T. B. Shows, H. Imura

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The human motilin gene has been isolated and characterized. The gene spans about 9 kilobase pairs (kb) and the 0.7 kb motilin mRNA is encoded by five exons. The 22-amino-acid motilin sequence is encoded by exons 2 and 3. The human motilin gene was mapped to the p21.2→p21.3 region of chromosome 6 by hybridization of the cloned cDNA to DNAs from a panel of reduced human-mouse somatic cell hybrids and by in situ hybridization to human prometaphase chromosomes. RNA blotting using RNA prepared from various regions of the human gastrointestinal tract revealed high levels of motilin mRNA in duodenum and lower levels in the antrum of the stomach; motilin mRNA could not be detected by this procedure in the esophagus, cardia of the stomach, descending colon or gallbladder.

Original languageEnglish (US)
Pages (from-to)248-252
Number of pages5
JournalFEBS letters
Volume249
Issue number2
DOIs
StatePublished - Jun 5 1989
Externally publishedYes

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Keywords

  • (Duodenum, Stomach, Human)
  • Chromosome 6
  • Motilin gene

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Yano, H., Seino, Y., Fujita, J., Yamada, Y., Inagaki, N., Takeda, J., Bell, G. I., Eddy, R. L., Fan, Y. S., Byers, M. G., Shows, T. B., & Imura, H. (1989). Exon-intron organization, expression, and chromosomal localization of the human motilin gene. FEBS letters, 249(2), 248-252. https://doi.org/10.1016/0014-5793(89)80633-7