Excitotoxicity and Metabolic Crisis Are Associated with Spreading Depolarizations in Severe Traumatic Brain Injury Patients

Jason M. Hinzman, J. Adam Wilson, Anna Teresa Mazzeo, Ross Bullock, Jed A. Hartings

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Cerebral microdialysis has enabled the clinical characterization of excitotoxicity (glutamate >10 μM) and non-ischemic metabolic crisis (lactate/pyruvate ratio [LPR] >40) as important components of secondary damage in severe traumatic brain injury (TBI). Spreading depolarizations (SD) are pathological waves that occur in many patients in the days following TBI and, in animal models, cause elevations in extracellular glutamate, increased anaerobic metabolism, and energy substrate depletion. Here, we examined the association of SD with changes in cerebral neurochemistry by placing a microdialysis probe alongside a subdural electrode strip in peri-lesional cortex of 16 TBI patients requiring neurosurgery. In 107 h (median; range: 76-117 h) of monitoring, 135 SDs were recorded in six patients. Glutamate (50 μmol/L) and lactate (3.7 mmol/L) were significantly elevated on day 0 in patients with SD compared with subsequent days and with patients without SD, whereas pyruvate was decreased in the latter group on days 0 and 1 (two-way analysis of variance [ANOVA], p values <0.05). In patients with SD, both glutamate and LPR increased in a dose-dependent manner with the number of SDs in the microdialysis sampling period (0, 1, ≥2 SD) [glutamate: 2.1→7.0→52.3 μmol/L; LPR: 27.8→29.9→45.0, p values <0.05]. In these patients, there was a 10% probability of SD occurring when glutamate and LPR were in normal ranges, but a 60% probability when both variables were abnormal (>10 μmol/L and >40 μmol/L, respectively). Taken together with previous studies, these preliminary clinical results suggest SDs are a key pathophysiological process of secondary brain injury associated with non-ischemic glutamate excitotoxicity and severe metabolic crisis in severe TBI patients.

Original languageEnglish (US)
Pages (from-to)1775-1783
Number of pages9
JournalJournal of Neurotrauma
Volume33
Issue number19
DOIs
StatePublished - Oct 1 2016

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Glutamic Acid
Microdialysis
Pyruvic Acid
Lactic Acid
Anaerobiosis
Neurochemistry
Neurosurgery
Brain Injuries
Traumatic Brain Injury
Analysis of Variance
Electrodes
Animal Models

Keywords

  • electrocorticography
  • multi-modal monitoring
  • spreading depression

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Excitotoxicity and Metabolic Crisis Are Associated with Spreading Depolarizations in Severe Traumatic Brain Injury Patients. / Hinzman, Jason M.; Wilson, J. Adam; Mazzeo, Anna Teresa; Bullock, Ross; Hartings, Jed A.

In: Journal of Neurotrauma, Vol. 33, No. 19, 01.10.2016, p. 1775-1783.

Research output: Contribution to journalArticle

Hinzman, Jason M. ; Wilson, J. Adam ; Mazzeo, Anna Teresa ; Bullock, Ross ; Hartings, Jed A. / Excitotoxicity and Metabolic Crisis Are Associated with Spreading Depolarizations in Severe Traumatic Brain Injury Patients. In: Journal of Neurotrauma. 2016 ; Vol. 33, No. 19. pp. 1775-1783.
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