Excitation-contraction coupling in skeletal muscle: Blockade by high extracellular concentrations of calcium buffers

J. N. Barrett; E. F. Barrett

doi:10.1126/science.96524

Excitation-contraction coupling in skeletal muscle: Blockade by high extracellular concentrations of calcium buffers

J. N. Barrett, E. F. Barrett

Physiology & Biophysics

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

High concentrations (80 to 90 millimolar) of the calcium buffers EGTA and citrate (less than 10^-7 molar free calcium ion) reversibly block excitation-contraction coupling in intact frog skeletal muscle fibers, but do not block caffeine-induced contractures. Solutions containing the same free calcium concentration but lower concentrations of calcium buffer (1 millimolar) do not block excitation-contraction coupling. These results suggest that excitation-contraction coupling requires the presence of calcium in a 'protected' extracellular compartment, probably the transverse tubular network, and that calcium is actively transported into this compartment from the muscle cell cytoplasm.

Original language	English (US)
Pages (from-to)	1270-1272
Number of pages	3
Journal	Science
Volume	200
Issue number	4347
DOIs	https://doi.org/10.1126/science.96524
State	Published - Jan 1 1978

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abstract = "High concentrations (80 to 90 millimolar) of the calcium buffers EGTA and citrate (less than 10-7 molar free calcium ion) reversibly block excitation-contraction coupling in intact frog skeletal muscle fibers, but do not block caffeine-induced contractures. Solutions containing the same free calcium concentration but lower concentrations of calcium buffer (1 millimolar) do not block excitation-contraction coupling. These results suggest that excitation-contraction coupling requires the presence of calcium in a 'protected' extracellular compartment, probably the transverse tubular network, and that calcium is actively transported into this compartment from the muscle cell cytoplasm.",

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AB - High concentrations (80 to 90 millimolar) of the calcium buffers EGTA and citrate (less than 10-7 molar free calcium ion) reversibly block excitation-contraction coupling in intact frog skeletal muscle fibers, but do not block caffeine-induced contractures. Solutions containing the same free calcium concentration but lower concentrations of calcium buffer (1 millimolar) do not block excitation-contraction coupling. These results suggest that excitation-contraction coupling requires the presence of calcium in a 'protected' extracellular compartment, probably the transverse tubular network, and that calcium is actively transported into this compartment from the muscle cell cytoplasm.

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