Ex vivo generation of functional immune cells by mitochondria-targeted photosensitization of cancer cells

Sean Marrache, Smanla Tundup, Donald A. Harn, Shanta Dhar

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Stimulating the immune system for potent immune therapy against cancer is potentially a revolutionary method to eradicate cancer. Tumors stimulated with photosensitizers (PSs) not only kill cancer cells but also help to boost the immune system. We recently reported that tumor-associated antigens (TAAs) generated by delivery of a mitochondria-acting PS zinc phthalocyanine (ZnPc) to MCF-7 breast cancer cells followed by laser irradiation can lead to ex vivo stimulation of mouse bone marrow-derived dendritic cells (BMDCs). The antigens generated from the breast cancer cells were also found to cause significant DC maturation and the activated DCs were able to stimulate T cells to cytotoxic CD8+ T cells. In this protocol, we describe methods to engineer a mitochondria-targeted biodegradable nanoparticle (NP) formulation, T-ZnPc-NPs for delivery of ZnPc to the mitochondria of MCF-7 cells, subsequent photodynamic therapy (PDT) using a long wavelength laser irradiation to produce TAAs, DC stimulation by the TAAs to secrete interferon-gamma (IFN-γ), and matured DC-driven T-cell activation.

Original languageEnglish (US)
Pages (from-to)113-122
Number of pages10
JournalMethods in Molecular Biology
Volume1265
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

    Fingerprint

Keywords

  • Biodegradable nanoparticle
  • Mitochondria
  • Photodynamic therapy
  • Tumor antigen

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this